Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,197,774 T→A 7.5% E270D (GAA→GAT)  PP_4578 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,197,7740TA 7.5% 146.4 / 2.5 53E270D (GAA→GAT) PP_4578MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (29/20);  new base A (2/2);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

TGACGAAAGAGCCCACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAGAAGCGCGAAGGTGCCTCGGC  >  NC_002947/5197629‑5197914
                                                                                                                                                 |                                                                                                                                            
tGACGAAAGAGCCCACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGcgttcg                                                                                                                                           >  3:97576/1‑149 (MQ=255)
  aCGAAAGAGCCCACGGCGAACAGGACGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGcgttcgtt                                                                                                                                         <  5:73163/149‑1 (MQ=255)
   cGAAAGAGCCCACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCggtcgttc                                                                                                                                        <  1:248595/149‑1 (MQ=255)
           cccACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGaa                                                                                                                                <  2:3203/149‑1 (MQ=255)
             cACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGcgttcgtt                                                                                                                                         <  4:233289/138‑1 (MQ=255)
             cACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGcgttcgtt                                                                                                                                         >  3:233289/1‑138 (MQ=255)
                    aaCAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAg                                                                                                                       >  6:12690/1‑149 (MQ=255)
                         gATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGtt                                                                                                                            <  8:190103/139‑1 (MQ=255)
                         gATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGGGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGtt                                                                                                                            >  7:190103/1‑139 (MQ=255)
                               gTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGa                                                                                                            >  4:145894/1‑149 (MQ=255)
                               gTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGa                                                                                                            >  8:80236/1‑149 (MQ=255)
                                      ttGCGCCCCAGCAGGTCGGAGAGATAAGCGCGGACCACATAGCCCAGGTATGAGCCGACGATCAACATGGCCTGATAGCCACCCGTGCCCCGCACGCTCAGGCAGCGTTGGGTCTTCAGGACGGTGGGCTGCGCCGTGGTGTTGTCg                                                                                                       >  4:225866/1‑147 (MQ=255)
                                           ccccAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAgc                                                                                                >  1:315575/1‑149 (MQ=255)
                                            cccAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAgcc                                                                                               >  7:98942/1‑149 (MQ=255)
                                                        gatagataAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGcccc                                                                                   >  8:227820/1‑149 (MQ=255)
                                                            gataAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCggtg                                                                               <  7:80236/149‑1 (MQ=255)
                                                            gataAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCggtg                                                                               <  5:12690/149‑1 (MQ=255)
                                                               aaGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGa                                                                                          >  6:211300/1‑135 (MQ=255)
                                                               aaGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGa                                                                                          <  5:211300/135‑1 (MQ=255)
                                                                 gcgcTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTcag                                                                          <  7:227820/149‑1 (MQ=255)
                                                                     tGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTgg                                                                                                                          >  6:35173/1‑97 (MQ=255)
                                                                     tGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTgg                                                                                                                          <  5:35173/97‑1 (MQ=255)
                                                                              aGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCg                                                              >  6:335774/1‑148 (MQ=255)
                                                                                cccATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGATCGTTTTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAgcg                                                                                       <  1:261930/121‑1 (MQ=255)
                                                                                cccATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGATCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAgcg                                                                                       <  1:257520/121‑1 (MQ=255)
                                                                                cccATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGATCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAgcg                                                                                       >  2:261930/1‑121 (MQ=255)
                                                                                cccATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGATCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAgcg                                                                                       >  2:257520/1‑121 (MQ=255)
                                                                                 ccATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGcaca                                                          <  3:145894/149‑1 (MQ=255)
                                                                                  cATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAg                                                         >  7:249403/1‑149 (MQ=255)
                                                                                               acgaTCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAATAGCCGCCGAGCGCCCCGGTGGTc                                                                            <  3:119546/117‑1 (MQ=255)
                                                                                               acgaTCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAATAGCCGCCGAGCGCCCCGGTGGTc                                                                            >  4:119546/1‑117 (MQ=255)
                                                                                                 gaTCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGc                                          >  6:159429/1‑149 (MQ=255)
                                                                                                     aCCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCg                                       <  4:97576/148‑1 (MQ=255)
                                                                                                       cATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCgggg                                    >  1:345404/1‑149 (MQ=255)
                                                                                                        aTGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCgtagta                                                                                                  <  4:239645/86‑1 (MQ=255)
                                                                                                        aTGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCgtagta                                                                                                  >  3:239645/1‑86 (MQ=255)
                                                                                                        aTGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGc                                   >  4:180010/1‑149 (MQ=255)
                                                                                                          ggCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCg                                  >  3:97158/1‑148 (MQ=255)
                                                                                                             cAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCg                                  >  8:276955/1‑145 (MQ=255)
                                                                                                              aGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTc                                                    <  5:81316/126‑1 (MQ=255)
                                                                                                              aGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTc                                                    >  6:81316/1‑126 (MQ=255)
                                                                                                               gATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCATCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGAtt                            <  2:315575/149‑1 (MQ=255)
                                                                                                               gATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGAtt                            >  4:285927/1‑149 (MQ=255)
                                                                                                                 tAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTc                          <  5:335774/149‑1 (MQ=255)
                                                                                                                   gCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGt                        <  8:249403/149‑1 (MQ=255)
                                                                                                                   gCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGt                        <  2:345404/149‑1 (MQ=255)
                                                                                                                    ccACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCg                         >  2:275175/1‑147 (MQ=255)
                                                                                                                      aCCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGGTGCCCGCGCAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAgc                     >  3:227098/1‑148 (MQ=255)
                                                                                                                      aCCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGcaca                                                          <  7:123495/112‑1 (MQ=255)
                                                                                                                      aCCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGcaca                                                          >  8:123495/1‑112 (MQ=255)
                                                                                                                               cAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGcaca                                                          <  2:243938/103‑1 (MQ=255)
                                                                                                                               cAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGcaca                                                          >  1:243938/1‑103 (MQ=255)
                                                                                                                               cAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAGAAGCGCGAAg            >  1:141831/1‑149 (MQ=255)
                                                                                                                                 ggaCGCTGAGGCCGGTTTCGTTCTTCAGGTAGGTTGGCAGCCACGTGGTGATGGCGTAGTAGCCGCCGAGCGCGCCGGTGGTGATCAGTGATGCCCGCACAGTGGTCCAGAGCATGCCCGTGGCGAAGATTTCGTAGAAGCGCGAAGGt          <  8:98942/147‑1 (MQ=255)
                                                                                                                                     gCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAGAAGCGCGAAGGTGCCt      >  3:141199/1‑149 (MQ=255)
                                                                                                                                     gCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAGAAGCGCGAAGGTGCCt      >  3:106905/1‑149 (MQ=255)
                                                                                                                                       tCAGTCCGCGTTCGTTCTTCAGGTAGGTTGGCAGCCTAGAGGTGATGTCGTAGTAGCCGCCTAGCGGCCCGTTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATTCCCGGGTAGAAGATTTCGTAGAAGCGCGAAGGTGCCTCg    <  4:97158/149‑1 (MQ=255)
                                                                                                                                         aGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAGAAGCGCGAAGGTGCCTCGGc  >  1:20960/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TGACGAAAGAGCCCACGGCGAACAGGATGAAGTTCTTCTTGCGCCCCAGCAGGTCGGATAGATAAGCGCTGACCACATAGCCCATGTATGAGCCGACGATCACCATGGCCAGATAGCCACCCGTGCCCAGCACGCTCAGGCCGCGTTCGTTCTTCAGGAAGGTTGGCAGCCACGAGGTGATGGCGTAGTAGCCGCCGAGCGCCCCGGTGGTCAGCAGGGATGCCCGCACAGTGGTCCAGAGCATGCCCGGGGCGAAGATTTCGTAGAAGCGCGAAGGTGCCTCGGC  >  NC_002947/5197629‑5197914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑