Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,211,028 T→C 11.6% I308V (ATC→GTC)  PP_4592 ← metal dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,211,0280TC11.6% 106.7 / 5.5 43I308V (ATC→GTC) PP_4592metal dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base T (20/18);  new base C (3/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAAGATCTGGTCATGGCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCCAGCAGGCCCTGCTCCCTGGC  >  NC_002947/5210880‑5211172
                                                                                                                                                    |                                                                                                                                                
agcaAGATCTGGTCATGGCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGAt                                                                                                                                                  >  8:179892/1‑149 (MQ=255)
  caAGATCTGGTCATGGCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGt                                                                                                                                                >  4:191846/1‑149 (MQ=255)
          ggTCATGGCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCTGGACATCCtgcagc                                                                                                                                        >  7:123782/1‑149 (MQ=255)
               tGGCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTc                                                                                                                                   <  7:179892/149‑1 (MQ=255)
                 gCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCTGGACATCCtgcagcgtgccg                                                                                                                                  <  8:123782/148‑7 (MQ=255)
                 gCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTcgc                                                                                                                                 <  4:108909/149‑1 (MQ=255)
                  cATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGATGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTcgcg                                                                                                                                >  1:234091/1‑149 (MQ=255)
                              gcgcACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTAc                                                                                                                    >  3:93021/1‑149 (MQ=255)
                                          gggTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTa                                                                                                        <  3:191846/149‑1 (MQ=255)
                                            gTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCTGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGt                                                                                                      >  6:253120/1‑149 (MQ=255)
                                              ccTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTcc                                                                                                    >  2:172587/1‑149 (MQ=255)
                                                ttGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCTGGACATCCtgcagcgt                                                                                                                                      <  5:188469/113‑3 (MQ=255)
                                                ttGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCTGGACATCCtgcagcgt                                                                                                                                      >  6:188469/1‑111 (MQ=255)
                                                  gAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCtt                                                                                                  <  4:93021/147‑1 (MQ=255)
                                                   aGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTgc                                                                                                <  2:234091/148‑1 (MQ=255)
                                                                 gATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAg                                                                                                       <  6:35863/127‑1 (MQ=255)
                                                                 gATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAg                                                                                                       >  5:35863/1‑127 (MQ=255)
                                                                   tGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCgg                                                                                                                <  2:189691/116‑1 (MQ=255)
                                                                   tGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCgg                                                                                                                >  1:189691/1‑116 (MQ=255)
                                                                            cgcgATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCgg                                                                                                                <  6:80260/107‑1 (MQ=255)
                                                                            cgcgATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCgg                                                                                                                >  5:80260/1‑107 (MQ=255)
                                                                              cgATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCTGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCAc                                                                    <  5:253120/149‑1 (MQ=255)
                                                                                         gCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAAccgccg                                                         >  6:245729/1‑149 (MQ=255)
                                                                                            tttGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGc                                                                                       <  8:57572/116‑1 (MQ=255)
                                                                                            tttGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGc                                                                                       >  7:57572/1‑116 (MQ=255)
                                                                                                   cccGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTAcc                                               <  8:107159/149‑1 (MQ=255)
                                                                                                           gTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGt                                       >  1:303668/1‑149 (MQ=255)
                                                                                                           gTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGt                                       >  3:71510/1‑149 (MQ=255)
                                                                                                               tCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCAc                                   >  7:299404/1‑149 (MQ=255)
                                                                                                                   gCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCg                               >  4:31388/1‑149 (MQ=255)
                                                                                                                      aCTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGaa                            <  2:303668/149‑1 (MQ=255)
                                                                                                                        tGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGaaaa                          >  7:303862/1‑149 (MQ=255)
                                                                                                                           aCGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGc                       <  8:299404/149‑1 (MQ=255)
                                                                                                                             ggCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCca                     <  4:71510/149‑1 (MQ=255)
                                                                                                                               ccAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTGATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTg                                      <  4:145595/130‑1 (MQ=255)
                                                                                                                                     cGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCCAGCAGGccc             >  8:97614/1‑149 (MQ=255)
                                                                                                                                      ggCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCcag                    <  7:177783/141‑1 (MQ=255)
                                                                                                                                      ggCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCcag                    >  8:177783/1‑141 (MQ=255)
                                                                                                                                         aCGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCCAGCAGGCCCTGCt         <  1:172587/149‑1 (MQ=255)
                                                                                                                                           gctgctGGACATCCTGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCACCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCCAGCAGGCCCTGCTcc       >  4:271472/1‑149 (MQ=255)
                                                                                                                                               cAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGa                                         <  3:181573/111‑1 (MQ=255)
                                                                                                                                               cAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGa                                         >  4:181573/1‑111 (MQ=255)
                                                                                                                                               cAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAAACGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACCCCAGCACGCCCTGCTCCCtcg   >  4:339275/1‑147 (MQ=255)
                                                                                                                                                 ggATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCCAGCAGGCCCTGCTCCCTGGc  >  8:249588/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCAAGATCTGGTCATGGCATTCGCCATCGGCGCACAGCTGCGGGTCCTTGAGGTTGCTGACGTCGATGAACTGCACGCGATCGCAGGTGCCTTTGCAGCCCGACTGGTTGTCACGCCACTGCACGGCCAGGCCGGCACGCTGCAGGATGTCCAGCAGCCCTTCGCGGTTCTTCGCTACGCGGGCATCGTAGTCCTTGCGCTTCATGCCCGAGAACATGCAAGGCACCGAAACCGCCGTTTCCGTACCGCACGAGTGCACGTCGGAAAACGCCAGCAGGCCCTGCTCCCTGGC  >  NC_002947/5210880‑5211172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑