Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 494,996 C→T 7.0% R253C (CGC→TGC)  djlA → DnaJ‑like protein DjlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947494,9960CT 7.0% 253.1 / 5.8 85R253C (CGC→TGC) djlADnaJ‑like protein DjlA
Reads supporting (aligned to +/- strand):  ref base C (40/39);  new base T (3/3);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

GATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCC  >  NC_002947/494849‑495143
                                                                                                                                                   |                                                                                                                                                    
gaTAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAgcggcg                                                                                                                                                     >  1:230727/1‑149 (MQ=255)
    aGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAgcggcgcggc                                                                                                                                                 <  7:277459/149‑1 (MQ=255)
     ggTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGcc                                                                                                                                                >  1:288012/1‑149 (MQ=255)
      gTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCt                                                                                                                                               >  1:126969/1‑149 (MQ=255)
      gTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCt                                                                                                                                               >  1:319853/1‑149 (MQ=255)
          aGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGa                                                                                                                                           <  3:297565/149‑1 (MQ=255)
           gCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGAc                                                                                                                                          <  5:118309/149‑1 (MQ=255)
                    tcgtcgGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGTCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCca                                                                                                                                 >  6:124527/1‑149 (MQ=255)
                    tcgtcgGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCca                                                                                                                                 <  5:152243/149‑1 (MQ=255)
                    tcggcgGCTTGTCCGCCGGCATCACCCGTACAAGCTGGCAGGTACCGGTGCCAGCTAGTCGCTGGTGCGTGAAGCAACCGCGCGTACCTGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGcc                                                                                                                                  <  8:220111/148‑1 (MQ=255)
                     cgtcgGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCca                                                                                                                                 >  8:285126/1‑148 (MQ=255)
                         ggCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAggg                                                                                                                            <  2:230727/149‑1 (MQ=255)
                         ggCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAggg                                                                                                                            <  2:319853/149‑1 (MQ=255)
                          gCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAgg                                                                                                                             >  7:194050/1‑147 (MQ=255)
                          gCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAgg                                                                                                                             <  8:194050/147‑1 (MQ=255)
                          gCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGa                                                                                                                           >  5:263059/1‑149 (MQ=255)
                                cAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGc                                                                                                                    >  7:123068/1‑149 (MQ=255)
                                   ccGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTg                                                                                                                 <  6:263059/149‑1 (MQ=255)
                                         tCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAg                                                                                                           >  1:143251/1‑149 (MQ=255)
                                                gACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGTCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAg                                                                                                    <  5:124527/149‑1 (MQ=255)
                                                gACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAg                                                                                                    <  7:285126/149‑1 (MQ=255)
                                                 aCAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGTGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAgg                                                                                                   >  4:21237/1‑149 (MQ=255)
                                                 aCAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAgg                                                                                                   >  4:71589/1‑149 (MQ=255)
                                                  cAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAggg                                                                                                  <  8:69331/149‑1 (MQ=255)
                                                   aaGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGc                                                                                                 <  2:288012/149‑1 (MQ=255)
                                                   aaGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGc                                                                                                 >  7:44931/1‑149 (MQ=255)
                                                    aGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGc                                                                                                          <  6:315426/139‑1 (MQ=255)
                                                    aGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGc                                                                                                          >  5:315426/1‑139 (MQ=255)
                                                     gCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAggg                                                                                                                            <  5:10412/121‑1 (MQ=255)
                                                     gCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAggg                                                                                                                            >  6:10412/1‑121 (MQ=255)
                                                     gCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCt                                                                                                                  >  3:227725/1‑130 (MQ=255)
                                                     gCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCt                                                                                                                  <  4:227725/130‑1 (MQ=255)
                                                      cTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCa                                                                                              <  3:126467/149‑1 (MQ=255)
                                                               gtacctGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCAccccc                                                                                     <  2:10452/144‑1 (MQ=255)
                                                                  ggTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCa                                                                                  <  8:44931/149‑1 (MQ=255)
                                                                   gTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGTGCGGCCTTTGACGCCCCGCCACAGGGa                                                                                                                           <  3:69186/108‑1 (MQ=255)
                                                                   gTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGTGCGGCCTTTGACGCCCCGCCACAGGGa                                                                                                                           >  4:73993/1‑108 (MQ=255)
                                                                   gTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGTGCGGCCTTTGACGCCCCGCCACAGGGa                                                                                                                           <  3:73993/108‑1 (MQ=255)
                                                                   gTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGTGCGGCCTTTGACGCCCCGCCACAGGGa                                                                                                                           >  4:69186/1‑108 (MQ=255)
                                                                   gTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAg                                                                                                                              <  4:291917/105‑1 (MQ=255)
                                                                      ccAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCcg                                                                              <  2:126969/149‑1 (MQ=255)
                                                                       cAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCcgg                                                                             >  7:106998/1‑149 (MQ=255)
                                                                             ggCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCAACCCCCGCACCCGGCGGac                                                                        >  5:298832/1‑147 (MQ=255)
                                                                                gcAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGa                                                                                                                >  4:191820/1‑105 (MQ=255)
                                                                                gcAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGa                                                                                                                <  3:191820/105‑1 (MQ=255)
                                                                                gcAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAt                                                                    >  7:11569/1‑149 (MQ=255)
                                                                                 cAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAtt                                                                   <  3:71589/149‑1 (MQ=255)
                                                                                           aaGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTc                                                         >  5:364036/1‑149 (MQ=255)
                                                                                             gCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAACTGCTCTTGTTccg                                                       >  3:112949/1‑149 (MQ=255)
                                                                                              cAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTccgc                                                      <  2:143251/149‑1 (MQ=255)
                                                                                               aaCCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTccgcc                                                     >  1:247355/1‑149 (MQ=255)
                                                                                               aaCCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAAGAGCAATTGTTccgcc                                                     <  8:123068/149‑1 (MQ=255)
                                                                                                 ccGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCcgccgc                                                   >  5:93475/1‑149 (MQ=255)
                                                                                                 ccGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAACTGCTCTTGTTCcgccgc                                                   <  4:112949/149‑1 (MQ=255)
                                                                                                 ccGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAACTGCTCTTGTTCcgccgc                                                   >  2:87899/1‑149 (MQ=255)
                                                                                                      cgTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCtttgt                                              <  6:93475/149‑1 (MQ=255)
                                                                                                       gTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCtttgtt                                             >  2:248345/1‑149 (MQ=255)
                                                                                                           ccGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGtt                                                          <  2:120084/132‑1 (MQ=255)
                                                                                                           ccGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCt                                         <  8:106998/149‑1 (MQ=255)
                                                                                                           ccGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCt                                         >  5:107801/1‑149 (MQ=255)
                                                                                                             gTGAGTTGCACCAGGCCTATGCGATGATCCGCAAGAGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCATGACTCAGCCCTGAGGGCTCATc                                                                                            >  7:220187/1‑96 (MQ=255)
                                                                                                               gAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGATGCAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtgg                                       >  1:278628/1‑148 (MQ=255)
                                                                                                               gAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggca                                     >  6:317578/1‑149 (MQ=255)
                                                                                                                 gTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatg                                   >  2:39550/1‑149 (MQ=255)
                                                                                                                  ttGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatgg                                  <  8:297032/149‑1 (MQ=255)
                                                                                                                      aCCAGGCCTATGCCATGATCCGCAAGCGGTGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaat                              <  3:21237/149‑1 (MQ=255)
                                                                                                                      aCCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTACCGCCGCTTTGTTTGCTGGCATGGcaa                               >  6:294165/1‑148 (MQ=255)
                                                                                                                        cAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatgg                                  <  1:4090/143‑1 (MQ=255)
                                                                                                                        cAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCtggcatgg                                  >  2:4090/1‑143 (MQ=255)
                                                                                                                        cAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatca                            <  2:102372/149‑1 (MQ=255)
                                                                                                                           gCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATg                         <  6:107801/149‑1 (MQ=255)
                                                                                                                           gCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATg                         <  2:247355/149‑1 (MQ=255)
                                                                                                                            ccTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGa                        <  5:317578/149‑1 (MQ=255)
                                                                                                                            ccTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGa                        <  1:248345/149‑1 (MQ=255)
                                                                                                                             cTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGa                        >  5:27612/1‑148 (MQ=255)
                                                                                                                                 gCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaatcagatccgg                     >  3:328105/1‑140 (MQ=255)
                                                                                                                                 gCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGcaat                              <  4:328105/138‑1 (MQ=255)
                                                                                                                                  ccATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTAACGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTg                  >  1:289937/1‑149 (MQ=255)
                                                                                                                                   cATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGctc                                                               <  3:345093/103‑1 (MQ=255)
                                                                                                                                   cATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGctc                                                               >  4:345093/1‑103 (MQ=255)
                                                                                                                                   cATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGt                 >  1:296624/1‑149 (MQ=255)
                                                                                                                                    aTGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTc                <  2:289937/149‑1 (MQ=255)
                                                                                                                                    aTGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTc                >  6:201857/1‑149 (MQ=255)
                                                                                                                                    aTGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTc                >  6:163833/1‑149 (MQ=255)
                                                                                                                                       aTCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCg               >  3:121531/1‑147 (MQ=255)
                                                                                                                                       aTCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCg               <  4:121531/147‑1 (MQ=255)
                                                                                                                                          cGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACt          >  4:70764/1‑149 (MQ=255)
                                                                                                                                               gcggcgcggcCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTa     >  6:62430/1‑149 (MQ=255)
                                                                                                                                               gcggcgcggcCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTa     >  5:31402/1‑149 (MQ=255)
                                                                                                                                                 ggcgcggcCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATc   <  6:27612/149‑1 (MQ=255)
                                                                                                                                                  gcgcggcCGGTGCCGCCCCGCCACAGGGCT‑CAAGCCTGACTCAGCCCTGAGGGCGCATCCACCCCCGCACCCGGCGGAACCATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATcc  <  6:298832/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTACCCGTGAGTTGCACCAGGCCTATGCCATGATCCGCAAGCGGCGCGGCCTTTGACGCCCCGCCACAGGGAT‑CAAGCCTGACTCAGCCCTGAGGGCTCATCCACCCCCGCACCCGGCGGAACAATTGCTCTTGTTCCGCCGCTTTGTTTGCTGGCATGGCAATCAATGACAATTGTCGGTACTGGCTATCC  >  NC_002947/494849‑495143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑