Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,235,123	A→G	6.8%	T153A (ACC→GCC)	fecA →	outer membrane ferric citrate porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,235,123	0	A	G	6.8%	168.3 / 2.2	59	T153A (ACC→GCC)	fecA	outer membrane ferric citrate porin
Reads supporting (aligned to +/- strand):  ref base A (28/27);  new base G (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

AGCCCCTGCTGCTGGCGCAGCAGTCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGACCGTGCTGAT  >  NC_002947/5234978‑5235262                                                                                                                                                   |                                                                                                                                            aGCCCCTGCTGCTGGCGCAGCAGTCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTacca                                                                                                                                          >  2:313196/1‑149 (MQ=255)           gctgGCGCAGCAGTCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCcgcg                                                                                                                                 >  8:280459/1‑148 (MQ=255)            ctgGCGCAGCAGTCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGaa                                                                                                                               <  7:280459/149‑1 (MQ=255)                 gcagcaGTCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCt                                                                                                                          >  8:126030/1‑149 (MQ=255)                        tCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCg                                                                                                                    >  6:359147/1‑148 (MQ=255)                          gAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCa                                                                                                                  <  1:313196/148‑1 (MQ=255)                            gCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAg                                                                                                                              >  2:93400/1‑134 (MQ=255)                            gCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAg                                                                                                                              <  1:93400/134‑1 (MQ=255)                             cTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTacca                                                                                                                                          >  8:98716/1‑121 (MQ=255)                              tAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATcccc                                                                                                             >  5:344925/1‑149 (MQ=255)                               aGGCGCCTCCACCGTGGTCGGCGACTGTCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGAACCCCGGCGCCCGCGACGTATTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACAGCCCGCGAAGTGCTCAACCGCATCCCCg                                                                                                            >  3:181442/1‑149 (MQ=255)                                   gcCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGt                                                                                                        >  8:110656/1‑149 (MQ=255)                                          ccGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGcc                                                                                                 >  4:4978/1‑149 (MQ=255)                                               gTCGGCGACTGGCTGGCCGATGCGTAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGa                                                                                            <  6:252197/149‑1 (MQ=255)                                                tCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGa                                                                                            >  6:117944/1‑148 (MQ=255)                                                  ggCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCaa                                                                                                     <  7:12909/137‑1 (MQ=255)                                                  ggCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCaa                                                                                                     >  8:12909/1‑137 (MQ=255)                                                   gCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGacaa                                                                                         >  4:96915/1‑148 (MQ=255)                                                     gACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGAcaacaa                                                                                      <  3:4978/149‑1 (MQ=255)                                                              gcCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCgg                                                                             >  3:312964/1‑149 (MQ=255)                                                                cGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACaacaac                                                                                     <  3:218315/139‑1 (MQ=255)                                                                cGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACaacaac                                                                                     >  4:218315/1‑139 (MQ=255)                                                                 gAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAg                                                                          <  5:117944/149‑1 (MQ=255)                                                                 gAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAg                                                                          >  4:243301/1‑149 (MQ=255)                                                                       cagcagGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGa                                                                    <  7:298772/149‑1 (MQ=255)                                                                        agcagGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGAc                                                                   >  1:8010/1‑149 (MQ=255)                                                                        agcagGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGAc                                                                   <  6:344925/149‑1 (MQ=255)                                                                        agcagGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGAc                                                                   <  3:96915/149‑1 (MQ=255)                                                                           agGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTg                                                                <  3:243301/149‑1 (MQ=255)                                                                            ggACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTgg                                                               <  7:110656/149‑1 (MQ=255)                                                                                aaCGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGaa                                                                                                                               <  4:128671/81‑1 (MQ=255)                                                                                aaCGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGaa                                                                                                                               >  3:128671/1‑81 (MQ=255)                                                                                 aCGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTg                                                          <  7:126030/149‑1 (MQ=255)                                                                                               cccGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCcg                                            >  4:336858/1‑149 (MQ=255)                                                                                               cccGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCcg                                            >  2:181951/1‑149 (MQ=255)                                                                                                ccGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGaa                                                        >  3:43191/1‑136 (MQ=255)                                                                                                ccGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGaa                                                        <  4:43191/136‑1 (MQ=255)                                                                                                 cGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCcgcg                                          >  2:91051/1‑149 (MQ=255)                                                                                                      cccGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTc                                     <  1:181951/149‑1 (MQ=255)                                                                                                       ccGCGACGTAGTGCGCCGTGAGGAATTGCGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTc                                                    >  1:15366/1‑134 (MQ=255)                                                                                                       ccGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTc                                                    <  2:15366/133‑1 (MQ=255)                                                                                                              gTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCcgcg                             >  1:6936/1‑149 (MQ=255)                                                                                                                  tGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTg                         <  2:8010/149‑1 (MQ=255)                                                                                                                    cgcCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTgg                        <  5:135688/148‑1 (MQ=255)                                                                                                                       cGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCTTCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTg                         <  8:160384/144‑1 (MQ=255)                                                                                                                       cGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCTTCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTg                         >  7:160384/1‑144 (MQ=255)                                                                                                                            ggAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCt               >  6:47448/1‑149 (MQ=255)                                                                                                                             gAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTc              >  2:167636/1‑149 (MQ=255)                                                                                                                               aTT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGa            <  5:47448/149‑1 (MQ=255)                                                                                                                                    gAACGCAACGGTGCTGCCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCt                                                                 <  2:203351/92‑1 (MQ=255)                                                                                                                                    gAACGCAACGGTGCTGCCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCt                                                                 <  2:208463/92‑1 (MQ=255)                                                                                                                                    gAACGCAACGGTGCTGCCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGCTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCt                                                                 >  1:208463/1‑92 (MQ=255)                                                                                                                                    gAACGCAACGGTGCTGCCACCGCCCGCGAAATGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCt                                                                 >  1:203351/1‑92 (MQ=255)                                                                                                                                    gAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCt                                      <  4:276780/119‑1 (MQ=255)                                                                                                                                    gAACGCAACGGTGCTACCACCGCCAGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCCCGCCTGGCAACGCGCTCGCCCGt        >  6:187913/1‑149 (MQ=255)                                                                                                                                     aaCGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGc                       <  1:347418/133‑1 (MQ=255)                                                                                                                                     aaCGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGc                       >  2:347418/1‑133 (MQ=255)                                                                                                                                     aaCGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGACCGTg       <  4:312964/149‑1 (MQ=255)                                                                                                                                          aaCGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACCACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGACCGTGCTgat  >  7:68495/1‑149 (MQ=255)                                                                                                                                          aaCGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGACCGTGCTgat  <  3:336858/149‑1 (MQ=255)                                                                                                                                          aaCGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGACCGTGCTgat  <  1:91051/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            AGCCCCTGCTGCTGGCGCAGCAGTCGAGCTAGGCGCCTCCACCGTGGTCGGCGACTGGCTGGCCGAGGCGCAGCAGGACAACGTATTCGAGCACCCCGGCGCCCGCGACGTAGTGCGCCGTGAGGAATT‑CGAACGCAACGGTGCTACCACCGCCCGCGAAGTGCTCAACCGCATCCCCGGGGTCAACGCCCCCGACAACAACGGTACCGGCAGCCATGACCTGGCGCTGAACTTCGGCATCCGCGGCCTCAACCCGCGCCTGGCATCGCGCTCGACCGTGCTGAT  >  NC_002947/5234978‑5235262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑