Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,253,887 G→C 11.6% F249L (TTC→TTG)  PP_4630 ← MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,253,8870GC11.6% 145.5 / 8.7 51F249L (TTC→TTG) PP_4630MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base C (2/4);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

GCCAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGAT  >  NC_002947/5253741‑5254033
                                                                                                                                                  |                                                                                                                                                  
gCCAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGaa                                                                                                                                                  <  3:172528/149‑1 (MQ=255)
     aaaCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGcc                                                                                                                                             <  5:326466/149‑1 (MQ=255)
           ccGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCGGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGa                                                                                                                                       <  7:188400/149‑1 (MQ=255)
           ccGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCACTTCAAGCGCCTGGCgt                                                                                                                                       <  2:22197/149‑2 (MQ=255)
             gCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGt                                                                                                                                     >  2:114601/1‑149 (MQ=255)
               aaGCGCTGGGTCATCACACTGCGCTGCGCGCCCTGGCAGGCTTCAGGGGTGCCAGCCATGCTGTCCTGTACCGCTTGCGCTGCTTCACCAGCCAGTACCATGGGGCATTCCAGCTTCTACGCCAGGCACTTCAAGCTGCTGGCgtcgat                                                                                                                                   <  4:54139/149‑6 (MQ=255)
               aaGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCACTTCAAGCGCCTGGCgtcgat                                                                                                                                   <  8:295461/149‑6 (MQ=255)
                 gcgcTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTctg                                                                                                                                 >  5:65121/1‑149 (MQ=255)
                       gcgcATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                            <  7:31571/148‑1 (MQ=255)
                          cATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                            >  1:79728/1‑145 (MQ=255)
                          cATCACACTGCACTGCTCGCCCAGGCAGGCTACAGGGCTGCCATCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                            <  2:79728/145‑1 (MQ=255)
                                acTCCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGGTGCTTCACCAGCCAGTAACAACGGGCATTCCAGGATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                  <  8:136266/149‑1 (MQ=255)
                                      cTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGaa                                                                                                             >  8:87705/1‑148 (MQ=255)
                                              ccAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCAc                                                                                                     >  4:57993/1‑148 (MQ=255)
                                                 ggcaggcTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAgg                                                                                                 <  8:16066/149‑1 (MQ=255)
                                                       cTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                            <  5:43083/116‑1 (MQ=255)
                                                       cTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                            >  6:43083/1‑116 (MQ=255)
                                                       cTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTcc                                                                                           <  7:87705/149‑1 (MQ=255)
                                                        tACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCCCCAGGGCGTCCg                                                                                          >  2:259324/1‑149 (MQ=255)
                                                        tACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCg                                                                                          >  3:308753/1‑149 (MQ=255)
                                                            gggCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCtt                                                                                      <  4:308753/149‑1 (MQ=255)
                                                              gCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCa                                                                                    <  8:167068/149‑1 (MQ=255)
                                                               cTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAg                                                                                   <  1:114601/149‑1 (MQ=255)
                                                                tGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGc                                                                                  >  1:164466/1‑149 (MQ=255)
                                                                    agccagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCGGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGtt                                                                              >  4:135644/1‑149 (MQ=255)
                                                                         ggCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCg                                                                            >  6:205877/1‑146 (MQ=255)
                                                                           cTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGAc                                                                       <  2:164466/149‑1 (MQ=255)
                                                                                   tACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCaa                                                               >  8:199526/1‑149 (MQ=255)
                                                                                                   tCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGACGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTg                                               >  7:49924/1‑149 (MQ=255)
                                                                                                     aCCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGc                                              <  6:65121/148‑1 (MQ=255)
                                                                                                       cagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCg                                           >  3:343018/1‑149 (MQ=255)
                                                                                                          ccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGa                                                                        >  1:1999/1‑117 (MQ=255)
                                                                                                          ccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGa                                                                        <  2:1999/117‑1 (MQ=255)
                                                                                                              tAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCa                                    <  5:205877/149‑1 (MQ=255)
                                                                                                                acaacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGc                                  >  4:172714/1‑149 (MQ=255)
                                                                                                                acaacGGGCATTCCAGAATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGAATGATGGGAAAACAGCACCAGGGCTTCCGGCTTCAGCCGATCGCACACCAGCGCAACCTCCTGCAGCCGCTTCCCGTTCGCCAGc                                  >  6:284477/1‑149 (MQ=255)
                                                                                                                  aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTc                                        >  5:59905/1‑141 (MQ=255)
                                                                                                                  aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTc                                        <  6:59905/141‑1 (MQ=255)
                                                                                                                  aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAg                                   <  8:34092/146‑1 (MQ=255)
                                                                                                                  aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAg                                   >  7:34092/1‑146 (MQ=255)
                                                                                                                  aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCgg                                >  5:346709/1‑149 (MQ=255)
                                                                                                                    cGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                  <  6:182077/65‑1 (MQ=255)
                                                                                                                    cGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                  >  5:182077/1‑65 (MQ=255)
                                                                                                                       gCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                            <  6:247758/132‑1 (MQ=255)
                                                                                                                       gCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                            >  5:247758/1‑132 (MQ=255)
                                                                                                                         aTTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACAAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCAcg                         >  2:126847/1‑149 (MQ=255)
                                                                                                                             cggCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCgt                      <  3:135644/146‑1 (MQ=255)
                                                                                                                                      cGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                            <  7:163363/117‑1 (MQ=255)
                                                                                                                                      cGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                            >  8:163363/1‑117 (MQ=255)
                                                                                                                                       gCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGGGTTTCGTCGCt           >  4:155314/1‑149 (MQ=255)
                                                                                                                                            gcgcTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCa      <  1:126847/149‑1 (MQ=255)
                                                                                                                                                  gAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGAt  <  7:199526/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCAGAAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGAT  >  NC_002947/5253741‑5254033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑