Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,253,891 T→C 10.5% H248R (CAC→CGC)  PP_4630 ← MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,253,8910TC10.5% 147.1 / 7.8 57H248R (CAC→CGC) PP_4630MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base C (2/4);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

AAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGC  >  NC_002947/5253746‑5254038
                                                                                                                                                 |                                                                                                                                                   
aaaCTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGcc                                                                                                                                                  <  5:326466/149‑1 (MQ=255)
      ccGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCGGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGa                                                                                                                                            <  7:188400/149‑1 (MQ=255)
      ccGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCACTTCAAGCGCCTGGCgt                                                                                                                                            <  2:22197/149‑2 (MQ=255)
        gCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGt                                                                                                                                          >  2:114601/1‑149 (MQ=255)
          aaGCGCTGGGTCATCACACTGCGCTGCGCGCCCTGGCAGGCTTCAGGGGTGCCAGCCATGCTGTCCTGTACCGCTTGCGCTGCTTCACCAGCCAGTACCATGGGGCATTCCAGCTTCTACGCCAGGCACTTCAAGCTGCTGGCgtcgat                                                                                                                                        <  4:54139/149‑6 (MQ=255)
          aaGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCACTTCAAGCGCCTGGCgtcgat                                                                                                                                        <  8:295461/149‑6 (MQ=255)
            gcgcTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTctg                                                                                                                                      >  5:65121/1‑149 (MQ=255)
                  gcgcATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                                 <  7:31571/148‑1 (MQ=255)
                     cATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                                 >  1:79728/1‑145 (MQ=255)
                     cATCACACTGCACTGCTCGCCCAGGCAGGCTACAGGGCTGCCATCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                                 <  2:79728/145‑1 (MQ=255)
                           acTCCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGGTGCTTCACCAGCCAGTAACAACGGGCATTCCAGGATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                       <  8:136266/149‑1 (MQ=255)
                                 cTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGaa                                                                                                                  >  8:87705/1‑148 (MQ=255)
                                         ccAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCAc                                                                                                          >  4:57993/1‑148 (MQ=255)
                                            ggcaggcTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAgg                                                                                                      <  8:16066/149‑1 (MQ=255)
                                                  cTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                                 <  5:43083/116‑1 (MQ=255)
                                                  cTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCtgtg                                                                                                                                 >  6:43083/1‑116 (MQ=255)
                                                  cTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTcc                                                                                                <  7:87705/149‑1 (MQ=255)
                                                   tACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCCCCAGGGCGTCCg                                                                                               >  2:259324/1‑149 (MQ=255)
                                                   tACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCg                                                                                               >  3:308753/1‑149 (MQ=255)
                                                       gggCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCtt                                                                                           <  4:308753/149‑1 (MQ=255)
                                                         gCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCa                                                                                         <  8:167068/149‑1 (MQ=255)
                                                          cTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAg                                                                                        <  1:114601/149‑1 (MQ=255)
                                                           tGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGc                                                                                       >  1:164466/1‑149 (MQ=255)
                                                               agccagGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCGGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGtt                                                                                   >  4:135644/1‑149 (MQ=255)
                                                                    ggCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCg                                                                                 >  6:205877/1‑146 (MQ=255)
                                                                      cTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGAc                                                                            <  2:164466/149‑1 (MQ=255)
                                                                              tACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCaa                                                                    >  8:199526/1‑149 (MQ=255)
                                                                                              tCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGACGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTg                                                    >  7:49924/1‑149 (MQ=255)
                                                                                                aCCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGc                                                   <  6:65121/148‑1 (MQ=255)
                                                                                                  cagccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCg                                                >  3:343018/1‑149 (MQ=255)
                                                                                                     ccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGa                                                                             <  2:1999/117‑1 (MQ=255)
                                                                                                     ccagTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGa                                                                             >  1:1999/1‑117 (MQ=255)
                                                                                                         tAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCa                                         <  5:205877/149‑1 (MQ=255)
                                                                                                           acaacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGc                                       >  4:172714/1‑149 (MQ=255)
                                                                                                           acaacGGGCATTCCAGAATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGAATGATGGGAAAACAGCACCAGGGCTTCCGGCTTCAGCCGATCGCACACCAGCGCAACCTCCTGCAGCCGCTTCCCGTTCGCCAGc                                       >  6:284477/1‑149 (MQ=255)
                                                                                                             aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTc                                             >  5:59905/1‑141 (MQ=255)
                                                                                                             aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTc                                             <  6:59905/141‑1 (MQ=255)
                                                                                                             aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAg                                        >  7:34092/1‑146 (MQ=255)
                                                                                                             aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAg                                        <  8:34092/146‑1 (MQ=255)
                                                                                                             aacGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCgg                                     >  5:346709/1‑149 (MQ=255)
                                                                                                               cGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                       <  6:182077/65‑1 (MQ=255)
                                                                                                               cGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGAt                                                                                                                       >  5:182077/1‑65 (MQ=255)
                                                                                                                  gCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                                 >  5:247758/1‑132 (MQ=255)
                                                                                                                  gCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                                 <  6:247758/132‑1 (MQ=255)
                                                                                                                    aTTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACAAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCAcg                              >  2:126847/1‑149 (MQ=255)
                                                                                                                        cggCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCgt                           <  3:135644/146‑1 (MQ=255)
                                                                                                                                 cGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                                 <  7:163363/117‑1 (MQ=255)
                                                                                                                                 cGCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGccc                                                 >  8:163363/1‑117 (MQ=255)
                                                                                                                                  gCCAGGCACTTCAAGCGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGGGTTTCGTCGCt                >  4:155314/1‑149 (MQ=255)
                                                                                                                                       gcgcTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCa           <  1:126847/149‑1 (MQ=255)
                                                                                                                                             gAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGAt       <  7:199526/147‑1 (MQ=255)
                                                                                                                                              aaGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCa     <  6:346709/148‑1 (MQ=255)
                                                                                                                                              aaGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCa     <  3:172714/148‑1 (MQ=255)
                                                                                                                                               aGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAgg   <  3:57993/149‑1 (MQ=255)
                                                                                                                                                gTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTtcgtc                   >  4:157599/1‑132 (MQ=255)
                                                                                                                                                gTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTtcgtc                   <  3:157599/132‑1 (MQ=255)
                                                                                                                                                gTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGc  >  3:178069/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AAACTGCCGCAAGCGCTGGCGCATCACACTGCCCTGCGCGCCCAGGCAGGCTACAGGGCTGCCAGCCAGGCTGTCCTGTACCGCTTCCGCTGCTTCACCAGCCAGTAACAACGGGCATTCCAGCATCGACGCCAGGCGCTTGAAGTGCCTGGCGAGTTCTGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGC  >  NC_002947/5253746‑5254038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑