Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,254,083 G→A 10.2% A184V (GCG→GTG)  PP_4630 ← MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,254,0830GA10.2% 103.4 / 2.3 39A184V (GCG→GTG) PP_4630MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (18/17);  new base A (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

GGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGCCGAACGACCATGTCATGCCAAACCGGCATGAA  >  NC_002947/5253937‑5254231
                                                                                                                                                  |                                                                                                                                                    
gggCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAACGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGgcgc                                                                                                                                                     >  7:229290/1‑148 (MQ=255)
  gCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCaa                                                                                                                                                  <  8:85085/149‑1 (MQ=255)
         gCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACg                                                                                                                                           <  1:259324/149‑1 (MQ=255)
                ccGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGc                                                                                                                                    >  7:118695/1‑149 (MQ=255)
                 cGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCt                                                                                                                                   <  8:229290/149‑1 (MQ=255)
                  gTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTa                                                                                                                                  >  5:219353/1‑149 (MQ=255)
                         gACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCa                                                                                                                                             >  8:307134/1‑131 (MQ=255)
                         gACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCa                                                                                                                                             <  7:307134/131‑1 (MQ=255)
                            cAGCGCAAGCTCCTGCAGCGGCCGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCGACACGGCacac                                                                                                                        >  1:239262/1‑149 (MQ=255)
                                 cAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACgg                                                                                                                      <  8:303853/146‑1 (MQ=255)
                                 cAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACgg                                                                                                                      >  7:303853/1‑146 (MQ=255)
                                         tGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCACCAATAGCACGGTGCGGCTACACgg                                                                                                                             >  1:4732/1‑131 (MQ=255)
                                         tGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCACCAATAGCACGGTGCGGCTACACgg                                                                                                                             <  2:4732/131‑1 (MQ=255)
                                            aGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCg                                                                                                        <  6:219353/149‑1 (MQ=255)
                                                      cGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCTCCGGCAATGGCGCCAATACCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAAcc                                                                                              >  8:323512/1‑149 (MQ=255)
                                                               gCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCgcgc                                                                                     <  6:264147/149‑1 (MQ=255)
                                                                   gATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAgg                                                                                  >  2:343692/1‑148 (MQ=255)
                                                                       aCGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCACCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGc                                                                                                         >  2:320001/1‑121 (MQ=255)
                                                                       aCGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCACCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGc                                                                                                         <  1:320001/121‑1 (MQ=255)
                                                                        cgcgTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGaa                                                                                                  <  5:151209/127‑1 (MQ=255)
                                                                        cgcgTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGaa                                                                                                  >  6:151209/1‑127 (MQ=255)
                                                                                          ccAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCg                                                          <  1:343692/149‑1 (MQ=255)
                                                                                            aGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTgt                                                                            >  4:24252/1‑128 (MQ=255)
                                                                                            aGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTgg                                                                            <  3:24252/129‑1 (MQ=255)
                                                                                                  aGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTg                                                  >  6:151598/1‑149 (MQ=255)
                                                                                                    gCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGTCTACACGGCACACGGGACAGCTGCAAGCGGGCGAAAACCCGACCTCGCAGGAACTGGTCATAGAACAGCCATTCTCTGGCCTGcc                                                <  6:57002/149‑1 (MQ=255)
                                                                                                    gCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTg                                                                             <  5:82000/120‑1 (MQ=255)
                                                                                                    gCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTg                                                                             >  6:82000/1‑120 (MQ=255)
                                                                                                        gTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCAt                                             <  8:118695/148‑1 (MQ=255)
                                                                                                         ttACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGt                                                                           <  6:322555/117‑1 (MQ=255)
                                                                                                         ttACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGt                                                                           >  5:322555/1‑117 (MQ=255)
                                                                                                             cAGCAACTCAAGCTCATGGCACTGCCCCTGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGc                                                     <  7:278083/135‑1 (MQ=255)
                                                                                                             cAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGc                                                     >  6:290272/1‑135 (MQ=255)
                                                                                                             cAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGc                                                     <  5:290272/135‑1 (MQ=255)
                                                                                                             cAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGc                                                     >  8:278083/1‑135 (MQ=255)
                                                                                                               gCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCt                                     >  4:138834/1‑149 (MQ=255)
                                                                                                                    tCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGcc                                 >  1:210145/1‑148 (MQ=255)
                                                                                                                            aTGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGCCGAACGACCa                        >  4:191416/1‑149 (MQ=255)
                                                                                                                                     ccccGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGCCGAACGACCATGTCATGc                >  1:168529/1‑148 (MQ=255)
                                                                                                                                     ccccGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGCCGAACGACCATGTCATGc                <  2:210145/148‑1 (MQ=255)
                                                                                                                                                  gcCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGCCGAACGACCATGTCATGCCAAACCGGCATGaa  <  3:138834/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCTTGCCGAACGACCATGTCATGCCAAACCGGCATGAA  >  NC_002947/5253937‑5254231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑