Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,341,584 G→T 7.7% V231L (GTG→TTG) ‡ panB → 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,341,5840GT 7.7% 163.3 / 4.3 52V231L (GTG→TTG) ‡panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (25/23);  new base T (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGG  >  NC_002947/5341439‑5341727
                                                                                                                                                 |                                                                                                                                               
cGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTga                                                                                                                                              <  8:13031/149‑1 (MQ=255)
 gTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                                                                                                >  7:216364/1‑146 (MQ=255)
 gTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGt                                                                                                                                                <  8:216364/146‑1 (MQ=255)
     ccAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAAc                                                                                                                                         >  1:56402/1‑149 (MQ=255)
                      gAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCATGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTgaaga                                                                                                                                           >  3:223499/1‑130 (MQ=255)
                      gAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCATGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTgaaga                                                                                                                                           <  4:223499/130‑1 (MQ=255)
                            aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                    <  1:326078/131‑3 (MQ=255)
                            aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                    >  2:326078/1‑129 (MQ=255)
                              cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                    <  4:113634/129‑3 (MQ=255)
                              cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                    >  3:113634/1‑127 (MQ=255)
                                aTGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTga                                                                                                                                              >  1:325463/1‑117 (MQ=255)
                                aTGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTga                                                                                                                                              <  2:325463/117‑1 (MQ=255)
                                         gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTg                                                                                                                                               <  3:261718/107‑1 (MQ=255)
                                         gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTg                                                                                                                                               >  4:261718/1‑107 (MQ=255)
                                         gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtc                                                                                                     >  7:205512/1‑149 (MQ=255)
                                            tCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                  <  2:56402/149‑1 (MQ=255)
                                               cGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCt                                                                                               <  8:205512/149‑1 (MQ=255)
                                                ggTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCt                                                                                               >  1:11166/1‑148 (MQ=255)
                                                 gTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGc                                                                                                >  6:229442/1‑146 (MQ=255)
                                                 gTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGc                                                                                                <  5:229442/146‑1 (MQ=255)
                                                                gcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa                                                                              <  2:11166/149‑1 (MQ=255)
                                                                gcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa                                                                              <  8:111187/149‑1 (MQ=255)
                                                                        cgcCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCa                                                                      <  2:320664/149‑1 (MQ=255)
                                                                            aCCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGttt                                                                  >  6:327696/1‑149 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCg                                                                                   >  2:72564/1‑124 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCg                                                                                   <  1:72564/124‑1 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                            <  7:226380/131‑1 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                            >  8:226380/1‑131 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                >  3:43985/1‑143 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                <  4:43985/143‑1 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                            >  2:177820/1‑147 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                            <  1:177820/147‑1 (MQ=255)
                                                                                      aGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGc                                                        <  5:327696/149‑1 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                                                                                                     <  7:64678/82‑1 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                                                                                                     >  8:64678/1‑82 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAacac                                                  >  6:291505/1‑149 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAacac                                                  >  3:117157/1‑149 (MQ=255)
                                                                                              gtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACgg                                                >  3:230320/1‑149 (MQ=255)
                                                                                                    cACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                             >  1:146836/1‑146 (MQ=255)
                                                                                                    cACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                             <  2:146836/146‑1 (MQ=255)
                                                                                                       gACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTg                                        >  2:124323/1‑148 (MQ=255)
                                                                                                         cATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                            <  1:312292/110‑1 (MQ=255)
                                                                                                         cATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                            >  2:312292/1‑110 (MQ=255)
                                                                                                             cTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGaa                                  >  5:48243/1‑148 (MQ=255)
                                                                                                                ggCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATa                                <  5:291505/147‑1 (MQ=255)
                                                                                                                    tGTCGCTGAGCGGCCGGGTACCGAAGAACGTGAAGAACTTCATGCAGGGCAAACCGGATATCCATAGCGACCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCAAGGCAGCGAACACTGGATCAGTGAATGAATACAGTc                           >  5:177939/1‑148 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTc                                            >  7:274187/1‑112 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTc                                            <  8:274187/112‑1 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGt                            >  3:12212/1‑128 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGt                            <  4:12212/128‑1 (MQ=255)
                                                                                                                                         cGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgcg     >  3:188123/1‑149 (MQ=255)
                                                                                                                                            aGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCgg  <  4:117157/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGG  >  NC_002947/5341439‑5341727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑