Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,341,586 G→C 9.1% V231V (GTG→GTC) ‡ panB → 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,341,5860GC 9.1% 132.3 / 4.1 44V231V (GTG→GTC) ‡panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/19);  new base C (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGTGG  >  NC_002947/5341439‑5341733
                                                                                                                                                   |                                                                                                                                                   
cGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTga                                                                                                                                                    <  8:13031/149‑1 (MQ=255)
     ccAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAAc                                                                                                                                               >  1:56402/1‑149 (MQ=255)
                      gAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCATGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTgaaga                                                                                                                                                 <  4:223499/130‑1 (MQ=255)
                      gAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCATGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTgaaga                                                                                                                                                 >  3:223499/1‑130 (MQ=255)
                            aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                          <  1:326078/131‑3 (MQ=255)
                            aCCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                          >  2:326078/1‑129 (MQ=255)
                              cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                          <  4:113634/129‑3 (MQ=255)
                              cAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCTTCACGAACTTcgg                                                                                                                                          >  3:113634/1‑127 (MQ=255)
                                aTGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTga                                                                                                                                                    >  1:325463/1‑117 (MQ=255)
                                aTGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTga                                                                                                                                                    <  2:325463/117‑1 (MQ=255)
                                         gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTg                                                                                                                                                     >  4:261718/1‑107 (MQ=255)
                                         gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTg                                                                                                                                                     <  3:261718/107‑1 (MQ=255)
                                         gCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtc                                                                                                           >  7:205512/1‑149 (MQ=255)
                                            tCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCtcgtc                                                                                                        <  2:56402/149‑1 (MQ=255)
                                               cGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCt                                                                                                     <  8:205512/149‑1 (MQ=255)
                                                ggTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCt                                                                                                     >  1:11166/1‑148 (MQ=255)
                                                 gTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGc                                                                                                      <  5:229442/146‑1 (MQ=255)
                                                 gTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGc                                                                                                      >  6:229442/1‑146 (MQ=255)
                                                                gcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa                                                                                    <  8:111187/149‑1 (MQ=255)
                                                                gcCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaa                                                                                    <  2:11166/149‑1 (MQ=255)
                                                                        cgcCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCa                                                                            <  2:320664/149‑1 (MQ=255)
                                                                            aCCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGttt                                                                        >  6:327696/1‑149 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCg                                                                                         >  2:72564/1‑124 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCg                                                                                         <  1:72564/124‑1 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                                  >  8:226380/1‑131 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                                  <  7:226380/131‑1 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                      >  3:43985/1‑143 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTcc                                                                      <  4:43985/143‑1 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                                  >  2:177820/1‑147 (MQ=255)
                                                                                    ccAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAgg                                                                  <  1:177820/147‑1 (MQ=255)
                                                                                      aGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGc                                                              <  5:327696/149‑1 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                                                                                                           <  7:64678/82‑1 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGa                                                                                                                           >  8:64678/1‑82 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAacac                                                        >  6:291505/1‑149 (MQ=255)
                                                                                            tggtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAacac                                                        >  3:117157/1‑149 (MQ=255)
                                                                                              gtactgCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACgg                                                      >  3:230320/1‑149 (MQ=255)
                                                                                                    cACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                                   >  1:146836/1‑146 (MQ=255)
                                                                                                    cACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGtt                                                   <  2:146836/146‑1 (MQ=255)
                                                                                                       gACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTg                                              >  2:124323/1‑148 (MQ=255)
                                                                                                         cATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                                  <  1:312292/110‑1 (MQ=255)
                                                                                                         cATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTcaagc                                                                                  >  2:312292/1‑110 (MQ=255)
                                                                                                             cTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGaa                                        >  5:48243/1‑148 (MQ=255)
                                                                                                                ggCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATa                                      <  5:291505/147‑1 (MQ=255)
                                                                                                                    tGTCGCTGAGCGGCCGGGTACCGAAGAACGTGAAGAACTTCATGCAGGGCAAACCGGATATCCATAGCGACCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCAAGGCAGCGAACACTGGATCAGTGAATGAATACAGTc                                 >  5:177939/1‑148 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTc                                                  <  8:274187/112‑1 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTc                                                  >  7:274187/1‑112 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGt                                  >  3:12212/1‑128 (MQ=255)
                                                                                                                                       aCCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGt                                  <  4:12212/128‑1 (MQ=255)
                                                                                                                                         cGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTgcgcg           >  3:188123/1‑149 (MQ=255)
                                                                                                                                            aGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCgg        <  4:117157/149‑1 (MQ=255)
                                                                                                                                                  tGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGTgg  <  4:188123/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGTACCCAGCGAACTGGCGGCGGAAATCACCAATGCCGTTGGCATCCCGGTAATCGGCATTGGCGCCGGCAGCGCCACCGATGGCCAGGTACTGGTACTGCACGACATGCTCGGCCTGTCGCTGAGCGGCCGGGTACCGAAGTTCGTGAAGAACTTCATGCAGGGCCAACCGGATATCCATAGCGCCCTCGTCGCTTACGTCGAGGCGGTCAAGCAAGTCAGTTTCCCAGGCAGCGAACACGGGTTCAGTGCATGAATACAGTCAAGACCGTCCGCGAACTGCGCGCGGCAGTGG  >  NC_002947/5341439‑5341733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑