Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,351,358 A→G 6.2% intergenic (+37/+39) PP_4706 → / ← PP_4707 hypothetical protein/OsmY‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,351,3580AG 6.2% 186.5 / 2.2 64intergenic (+37/+39)PP_4706/PP_4707hypothetical protein/OsmY‑like protein
Reads supporting (aligned to +/- strand):  ref base A (30/30);  new base G (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

CGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAACCTTCACATCAGAAGACA  >  NC_002947/5351212‑5351506
                                                                                                                                                   |                                                                                                                                                      
cgcATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAATAAAGCCCTGGCACGGGCCAGGGCTTCGAt                                                                                                                                                       <  1:26673/149‑1 (MQ=255)
     aCCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGACTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGt                                                                                                                                               >  2:256154/1‑149 (MQ=255)
      cccAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTg                                                                                                                                              <  4:55858/149‑1 (MQ=255)
       ccAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGaa‑‑a                                                                                                                                                 >  2:295611/1‑145 (MQ=255)
       ccAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGaa‑‑a                                                                                                                                                 <  1:295611/145‑1 (MQ=255)
              gcgcATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATc                                                                                                                                      >  6:140327/1‑149 (MQ=255)
                gcATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGt                                                                                                                                               <  4:117610/138‑1 (MQ=255)
                gcATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGt                                                                                                                                               >  3:117610/1‑138 (MQ=255)
                gcATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCa                                                                                                                                    <  8:108870/149‑1 (MQ=255)
                        gcgACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGcc                                                                                                                             >  3:36897/1‑148 (MQ=255)
                              gCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGa                                                                                                                      <  6:35285/149‑1 (MQ=255)
                                    cATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTcc                                                                                                                <  1:186015/149‑1 (MQ=255)
                                     aTTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGa                                                                                                                                                        >  3:327972/1‑110 (MQ=255)
                                     aTTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGa                                                                                                                                                        <  4:327972/110‑1 (MQ=255)
                                         gtggAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTAtt                                                                                                           >  1:343423/1‑149 (MQ=255)
                                              gCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTc                                                                                                          >  3:33492/1‑145 (MQ=255)
                                              gCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTc                                                                                                          <  4:33492/145‑1 (MQ=255)
                                                 tctGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCttt                                                                                                    >  6:102167/1‑148 (MQ=255)
                                                 tctGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCttt                                                                                                    >  7:23259/1‑148 (MQ=255)
                                                  ctGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCGATTTGGCCGACGGGACATTCCTTATTCGGCttt                                                                                                    >  5:112151/1‑147 (MQ=255)
                                                   tGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTc                                                                                                   <  1:221660/147‑1 (MQ=255)
                                                         ttGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGt                                                                                            >  1:349056/1‑148 (MQ=255)
                                                           gAGCAGGAATGATTCTAAAGCTATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTc                                                                                           <  6:112151/147‑1 (MQ=255)
                                                               aGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTc                                                                                           >  3:315501/1‑143 (MQ=255)
                                                               aGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTc                                                                                           <  4:315501/143‑1 (MQ=255)
                                                               aGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACAt                                                                                     >  4:52492/1‑149 (MQ=255)
                                                                    tGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGc                                                                                >  5:207715/1‑149 (MQ=255)
                                                                      cTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGctc                                                                              >  1:176395/1‑149 (MQ=255)
                                                                       ttCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGctct                                                                             >  5:174767/1‑149 (MQ=255)
                                                                         cGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCtt                                                                            <  5:194213/148‑1 (MQ=255)
                                                                         cGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCtt                                                                            <  2:343423/148‑1 (MQ=255)
                                                                           aaaGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGAc                                                                         >  8:284109/1‑149 (MQ=255)
                                                                               cGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCgg                                                                                                        >  8:207745/1‑114 (MQ=255)
                                                                               cGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCgg                                                                                                        <  7:207745/114‑1 (MQ=255)
                                                                                gATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCtt                                                                    >  3:42970/1‑149 (MQ=255)
                                                                                  tgtgATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTg                                                                  <  3:52492/149‑1 (MQ=255)
                                                                                     gATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGAt                                                                <  2:176395/148‑1 (MQ=255)
                                                                                     gATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATg                                                               >  5:54066/1‑149 (MQ=255)
                                                                                          cgcgcAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAAGTAGTGCCCAAATCCATTTGCCCGACGGGACATTCCTAATACGGCTTTCAGGCCGTCAGACATTACGCACTTGACGCCTTTGATGTTc                                                            >  1:274649/1‑149 (MQ=255)
                                                                                           gcgcAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACgg                                                                                                                        <  4:45728/86‑1 (MQ=255)
                                                                                           gcgcAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACgg                                                                                                                        >  3:45728/1‑86 (MQ=255)
                                                                                           gcgcAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg                                                         <  6:54066/149‑1 (MQ=255)
                                                                                           gcgcAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg                                                         <  6:207715/149‑1 (MQ=255)
                                                                                            cgcAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTgg                                                        <  5:102167/149‑1 (MQ=255)
                                                                                                 cccGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAAt                                                    >  4:342202/1‑148 (MQ=255)
                                                                                                    ggTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATgcgc                                                <  8:23259/149‑1 (MQ=255)
                                                                                                             aGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTc                                        <  6:174767/148‑1 (MQ=255)
                                                                                                              gcgcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCt                                       <  2:349056/148‑1 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGGTGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg                                                         >  1:164299/1‑128 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGGTGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg                                                         >  7:252280/1‑128 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGGTGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg                                                         <  2:164299/128‑1 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGGTGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTg                                                         <  8:252280/128‑1 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTgg                                                        >  8:201890/1‑129 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTgg                                                        <  7:201890/129‑1 (MQ=255)
                                                                                                                gcAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCt                                    >  8:2846/1‑149 (MQ=255)
                                                                                                                  aaGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGATGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCttt                                  >  3:90174/1‑149 (MQ=255)
                                                                                                                  aaGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCtt                                   >  6:321301/1‑148 (MQ=255)
                                                                                                                  aaGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCtt                                   >  8:200261/1‑148 (MQ=255)
                                                                                                                      a‑aaaGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGc                              <  4:42970/149‑1 (MQ=255)
                                                                                                                           gCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGAtt                          <  1:256154/149‑1 (MQ=255)
                                                                                                                             ccTGGCCCGTGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCg                        >  6:86785/1‑149 (MQ=255)
                                                                                                                              cTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGAt                                                                <  1:130597/108‑1 (MQ=255)
                                                                                                                              cTGGCACGGGCCAGGGATTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTAAGACATTAAGCACTTGACGCCTTTGAt                                                                >  2:130597/1‑108 (MQ=255)
                                                                                                                                  cACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAAc                   <  7:2846/149‑1 (MQ=255)
                                                                                                                                             gCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAACCTTCACATCag        <  7:200261/149‑1 (MQ=255)
                                                                                                                                                 cGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAACCTTCACATCagaa      <  5:321301/147‑1 (MQ=255)
                                                                                                                                                   aTGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAACCTTCACATCAGAAGACa  <  3:342202/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CGCATACCCAGACTGCGCATTTTCGCGACAGCCGTCCATTGGTGGAGCCTCTGATCCTTGAGCAGGAATGCTTCGAAAGCGATGTGATCGCGCGCAACCCGGTGTATTGAGCGCAAGAA‑AAAGCCCTGGCACGGGCCAGGGCTTCGATGAA‑‑AGTGGCCAAATCCATTTGGCCGACGGGACATTCCTTATTCGGCTTTCAGGCCGTCAGACATTACGCTCTTGACGCCTTTGATGTTCTTGGTAATGCGCACCGCTTCTGCTTTCTGCGATTCGGTAACCTTCACATCAGAAGACA  >  NC_002947/5351212‑5351506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑