| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,362,216 | C→G | 11.3% | Q48H (CAG→CAC) | tpiA ← | triose phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,362,216 | 0 | C | G | 11.3% | 125.1 / 5.4 | 43 | Q48H (CAG→CAC) | tpiA | triose phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (21/17); new base G (2/3); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAT > NC_002947/5362078‑5362359 | gCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCAGGTGATtgatgg < 1:223003/149‑2 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCAGGTGATtgatggc < 1:177705/142‑3 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCAGGTGATtgatggc > 2:177705/1‑140 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCtgattgat > 4:30011/1‑139 (MQ=255) acacCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCtgattgat < 3:30011/139‑1 (MQ=255) cAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTg > 4:69108/1‑149 (MQ=255) gCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTgg > 4:325266/1‑144 (MQ=255) gCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTgg < 3:325266/144‑1 (MQ=255) ttCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCtgattgat < 8:211574/120‑1 (MQ=255) ttCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCtgattgat > 7:211574/1‑120 (MQ=255) cAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACtt < 4:168926/147‑1 (MQ=255) tCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTc < 7:147533/147‑1 (MQ=255) tCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTc > 8:147533/1‑147 (MQ=255) gCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGAGCCGACAGTAATTTCTTTACCTGCAAGGCCAACAATCACCTGATTGATGAACAAGGCCGGTCGAAATACgg > 2:154696/1‑124 (MQ=255) cAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTGCAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTc > 6:25095/1‑130 (MQ=255) cAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTGCAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTc < 5:25095/130‑1 (MQ=255) aaCTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCga < 6:47856/149‑1 (MQ=255) cTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCgaga < 7:113503/149‑1 (MQ=255) ccGGTCAGCGCACCCTGTTCGGGTTGTAAAGCAGAATTCTGTGCGCCGTCAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACt > 6:75236/1‑149 (MQ=255) cccTGTTCGGGTTGTAAAGCAGAATTCTGTGCGCCGTCAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGt < 5:75236/149‑1 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCaa > 2:46665/1‑139 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCaa < 1:46665/139‑1 (MQ=255) ccTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGt > 2:282199/1‑148 (MQ=255) tgTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCTGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTg < 6:249410/137‑1 (MQ=255) ggTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTg > 5:249410/1‑138 (MQ=255) agcagAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAg > 5:1407/1‑149 (MQ=255) cagAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTc < 4:325055/119‑1 (MQ=255) cagAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTc > 3:325055/1‑119 (MQ=255) ttCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCggg > 2:113628/1‑148 (MQ=255) ttCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCggg > 7:20292/1‑148 (MQ=255) tgCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGt > 5:213485/1‑149 (MQ=255) aGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTcc < 8:20292/149‑1 (MQ=255) gTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCa < 3:69108/149‑1 (MQ=255) tCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTAc > 3:63983/1‑148 (MQ=255) ccTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTa < 6:1407/148‑1 (MQ=255) cTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTAc > 1:34346/1‑148 (MQ=255) gCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCa > 7:179375/1‑148 (MQ=255) cttgttcATCAATCAGGTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTc < 7:126703/125‑1 (MQ=255) cttgttcATCAATCAGGTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTc > 8:126703/7‑131 (MQ=255) aGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAgg > 6:77060/1‑149 (MQ=255) ggCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCgg < 4:91083/113‑1 (MQ=255) ggCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCgg > 3:91083/1‑113 (MQ=255) atcaatcaCCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGAc < 6:213485/149‑1 (MQ=255) cTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAt > 6:222386/1‑144 (MQ=255) cTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAt < 5:222386/144‑1 (MQ=255) | GCCAATCAACACCAACTTGCAACCTGCTTCAACCAGCTGCTCGGGAGCAACTTCCCCGGTCAGCGCACCCTGTTCGGGTTGTACAGCAGAATTCTGTGCGCCGACAGTAATTTCTTTACCTGCCAGGCCATCAATCACCTGATTGATGAACAAGGCCGGTGGAAATACGGCGACTTCCACTCCGCTCGGCAAGGCGAGATTACTCAAGCCTTTGGTCAGCTCAGCGACGCTAGCGCGGGTACCGTGCATTTTCCAGTTACCAGCTACCATAGGGCGACGCAT > NC_002947/5362078‑5362359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |