Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,364,420	C→G	6.5%	G72R (GGT→CGT)	folP ←	dihydropteroate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,364,420	0	C	G	6.5%	245.4 / 7.0	77	G72R (GGT→CGT)	folP	dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (34/38);  new base G (2/3);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

AGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGA  >  NC_002947/5364275‑5364563                                                                                                                                                   |                                                                                                                                                aGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCgg                                                                                                                                              >  2:263404/1‑149 (MQ=255)    ccGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGgcg                                                                                                                                           >  8:15554/1‑149 (MQ=255)       gCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGt                                                                                                                                        <  2:256716/149‑1 (MQ=255)           ccAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGAc                                                                                                                                    >  3:118853/1‑149 (MQ=255)             aGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCCCGGGTGCGCGTgcagtc                                                                                                                                  <  3:305748/149‑6 (MQ=255)             aGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCCCGGGTGCGCGTgcagtc                                                                                                                                  <  7:118546/149‑6 (MQ=255)             aGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTc                                                                                                                                  <  4:118853/149‑1 (MQ=255)              ggCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCg                                                                                                                                 >  6:334561/1‑149 (MQ=255)               gcgcgcGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGc                                                                                                                                <  5:334561/149‑1 (MQ=255)                           aCGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTg                                                                                                                                       >  6:361204/1‑130 (MQ=255)                           aCGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTg                                                                                                                                       <  5:361204/130‑1 (MQ=255)                           aCGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGa                                                                                                                                     <  4:55638/132‑1 (MQ=255)                           aCGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGa                                                                                                                                     >  3:55638/1‑132 (MQ=255)                           aCGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGa                                                                                                                    <  4:266701/149‑1 (MQ=255)                            cGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGAt                                                                                                                   <  1:263404/149‑1 (MQ=255)                             gCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATc                                                                                                                  <  5:58685/149‑1 (MQ=255)                                  aCAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCCCGGGTGCGCGTg                                                                                                                                       >  4:219793/1‑123 (MQ=255)                                  aCAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCCCGGGTGCGCGTg                                                                                                                                       <  3:219793/123‑1 (MQ=255)                                          cGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCcgg                                                                                                     >  7:297666/1‑149 (MQ=255)                                                   aTCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGcc                                                                                                       >  5:145833/1‑138 (MQ=255)                                                   aTCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGcc                                                                                                       <  6:145833/138‑1 (MQ=255)                                                                aCCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGt                                                                                                                                        >  5:83589/1‑92 (MQ=255)                                                                aCCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGt                                                                                                                                        <  6:83589/92‑1 (MQ=255)                                                                 ccACGTCGAGGCGACTGTTCATGGCCTCGACCATTGGCGCCACACGCTCCAGCTGCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTgg                                                                              <  6:108340/149‑1 (MQ=255)                                                                     gTCGAGGCGACTGTTGATGGCCTCGACCATAGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGgcg                                                                            >  1:31434/1‑147 (MQ=255)                                                                       cGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAgg                                                                        <  8:249496/149‑1 (MQ=255)                                                                        gAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAggg                                                                       >  8:197560/1‑149 (MQ=255)                                                                             gACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCCCCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTAGGCGTGGCGCAGGGCCtcg                                                                  >  6:34273/1‑149 (MQ=255)                                                                               cTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtcgtc                                                                <  5:185620/149‑1 (MQ=255)                                                                                tGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtcgtcg                                                               >  6:335853/1‑149 (MQ=255)                                                                                tGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtcgtcg                                                               >  5:279157/1‑149 (MQ=255)                                                                                  ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGatat                                                                                                                       <  4:277038/91‑1 (MQ=255)                                                                                  ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGatat                                                                                                                       >  3:277038/1‑91 (MQ=255)                                                                                  ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGc                                                                      <  3:105720/140‑1 (MQ=255)                                                                                  ttGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGc                                                                      >  4:105720/1‑140 (MQ=255)                                                                                    gATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCgcgc                                                            <  7:135183/148‑1 (MQ=255)                                                                                        gCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctgact                                                       >  3:149209/1‑149 (MQ=255)                                                                                         ccTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                         <  2:313909/146‑1 (MQ=255)                                                                                         ccTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                         >  1:313909/1‑146 (MQ=255)                                                                                          cTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTAGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctgactg                                                      >  7:128913/1‑148 (MQ=255)                                                                                           gcGACCATGCGCGCCACCCGCTCCAGCTCCGCGGTCACCTAGACTGC‑ACGCGCACCCGGGCGCTTGGACTCGCCACCGATATCGATCAACGTTGCTCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGaa                                                    <  8:249805/148‑1 (MQ=255)                                                                                             gACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGaa                                                    <  7:15554/147‑1 (MQ=255)                                                                                             gACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGaa                                                    <  5:335853/147‑1 (MQ=255)                                                                                                  ttGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCg                                             >  1:177268/1‑149 (MQ=255)                                                                                                    ggCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGcc                                                                     >  4:219131/1‑123 (MQ=255)                                                                                                    ggCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGcc                                                                     <  3:219131/123‑1 (MQ=255)                                                                                                     gcgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCa                                          >  6:277938/1‑149 (MQ=255)                                                                                                      cgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCAt                                         <  5:277938/149‑1 (MQ=255)                                                                                                       gcCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCTCCATc                                        <  7:197560/149‑1 (MQ=255)                                                                                                            acGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGgaga                                   <  2:31434/149‑1 (MQ=255)                                                                                                             cGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGaa                                  <  6:158215/149‑1 (MQ=255)                                                                                                             cGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGaa                                  <  6:279157/149‑1 (MQ=255)                                                                                                              gCTCCAGCTCCTCGGTCACCGAGACTGCGACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGgag                                    >  1:20714/1‑147 (MQ=255)                                                                                                                tCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAgg                                >  7:201064/1‑148 (MQ=255)                                                                                                                    gCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATc                                        <  8:168084/136‑1 (MQ=255)                                                                                                                    gCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATc                                        >  7:168084/1‑136 (MQ=255)                                                                                                                        ctcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCTGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                       >  4:177820/1‑149 (MQ=255)                                                                                                                        ctcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                       <  2:20714/149‑1 (MQ=255)                                                                                                                        ctcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgggg                        <  8:297666/148‑1 (MQ=255)                                                                                                                        ctcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCgggg                        <  2:177268/148‑1 (MQ=255)                                                                                                                        ctcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCg                           >  7:252832/1‑145 (MQ=255)                                                                                                                        ctcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCg                           <  8:252832/145‑1 (MQ=255)                                                                                                                         tcGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGt                      >  4:92896/1‑149 (MQ=255)                                                                                                                           ggTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGt                      <  4:149209/147‑1 (MQ=255)                                                                                                                               aCCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATAtt                <  1:136338/149‑1 (MQ=255)                                                                                                                                 cGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACGAAAGCATAGGCGTGGCGCAAGGCCAACTAGCGCTGACGGAAACGACCGCCAAAGGAGAAGGAATCGGGGGTGATAttt               >  1:281949/1‑147 (MQ=255)                                                                                                                                 cGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATAtt                >  2:300374/1‑147 (MQ=255)                                                                                                                                 cGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTga              <  8:201064/149‑1 (MQ=255)                                                                                                                                       tcc‑ACGCGCCCGGGTGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATAc        >  6:92395/3‑149 (MQ=255)                                                                                                                                       tGC‑ACGCGCACCCGGGCGCGTGGACTCGCGACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGAGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGCGAAGGAATCGGGGGTGATATTGAGAATAc        >  6:276323/1‑149 (MQ=255)                                                                                                                                       tGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATAc        >  8:239814/1‑149 (MQ=255)                                                                                                                                        gC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                         <  7:101933/99‑1 (MQ=255)                                                                                                                                        gC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                         >  8:101933/1‑99 (MQ=255)                                                                                                                                            cgcgCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTAGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt    <  8:128913/149‑1 (MQ=255)                                                                                                                                              cgcACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGa  <  1:300374/149‑1 (MQ=255)                                                                                                                                               gcACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGa                                                >  4:214622/1‑102 (MQ=255)                                                                                                                                               gcACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGa                                                <  3:214622/102‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                AGCCCGGCCCCCAGGCGCGCGGCTTCACGCATGACAGCAGGCGTGGAGGTATCGACCGAAATGACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGC‑ACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGA  >  NC_002947/5364275‑5364563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑