| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 500,548 | C→T | 10.2% | A366V (GCG→GTG) | PP_0411 → | polyamine ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 500,548 | 0 | C | T | 10.2% | 133.2 / 4.7 | 49 | A366V (GCG→GTG) | PP_0411 | polyamine ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (21/23); new base T (3/2); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
ATTTACCTGGGCGACCACGTGCGGGTGCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATGGCCGCAGAC > NC_002947/500403‑500688 | aTTTACGTGGGCTAGCACGTGCGGGTGCGTCTGGAGGTTTGCGGCATAGGCGACTTCTTCGTGAAGCAGCGGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGAGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCt < 8:199622/149‑1 (MQ=255) ccTGGGCGACCACGTGCGGGTGCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑c > 2:134126/1‑149 (MQ=255) ggCGACCACGTGCGGGTGCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTTGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGa < 1:21315/148‑1 (MQ=255) aCGTGCGGGTGCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCg > 3:344279/1‑147 (MQ=255) tGCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGa < 1:134126/149‑1 (MQ=255) gCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGAt > 8:261084/1‑149 (MQ=255) gAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGc > 4:96956/1‑149 (MQ=255) ggTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCtt < 3:96956/149‑1 (MQ=255) gTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCtt > 3:81487/1‑148 (MQ=255) ttGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGc < 5:99313/129‑1 (MQ=255) ttGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGc > 6:99313/1‑129 (MQ=255) gCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCAcc < 8:300306/148‑1 (MQ=255) cAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAAccc > 8:22147/1‑149 (MQ=255) gCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTAc < 7:261084/149‑1 (MQ=255) aCTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGATCCCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACtg < 1:72790/149‑1 (MQ=255) gTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGgagaga < 6:101843/148‑1 (MQ=255) gTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGaa > 7:268923/1‑149 (MQ=255) tGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGaat < 8:268923/149‑1 (MQ=255) cagcCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACtgt > 1:156110/1‑135 (MQ=255) cagcCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACtgt < 2:156110/135‑1 (MQ=255) gcCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATg < 4:81487/149‑1 (MQ=255) gATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGaataataa < 7:129616/144‑1 (MQ=255) gATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGaataataa > 8:129616/1‑144 (MQ=255) ttGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATgcgc > 5:23649/1‑147 (MQ=255) gAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGaataa > 2:242154/1‑134 (MQ=255) gAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGaataa < 1:242154/134‑1 (MQ=255) ccGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGa > 5:49035/1‑70 (MQ=255) ccGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGa < 6:49035/70‑1 (MQ=255) cGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGAc > 6:144606/1‑149 (MQ=255) gCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTTGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTg < 7:22147/148‑1 (MQ=255) tGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGgag < 8:34598/105‑1 (MQ=255) tGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGgag > 7:34598/1‑105 (MQ=255) ggCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTg < 6:23649/148‑1 (MQ=255) gCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTggg > 1:206569/1‑149 (MQ=255) tagtaCCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGtt < 2:206569/149‑1 (MQ=255) taCCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCAc > 2:52962/1‑149 (MQ=255) cGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGc > 8:198282/1‑149 (MQ=255) ggctgggAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTggc < 6:233118/148‑1 (MQ=255) tgggAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTggccatg > 8:178392/1‑147 (MQ=255) gTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATgg < 4:344279/149‑1 (MQ=255) atcgAGCGCGCGGGCGTGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTgggg < 8:88294/121‑1 (MQ=255) atcgAGCGCGCGGGCGTGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTgggg > 7:88294/4‑124 (MQ=255) atcgAGCGCGCGGGCGTGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTg > 3:43470/4‑121 (MQ=255) atcgAGCGCGCGGGCGTGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTg < 4:43470/118‑1 (MQ=255) cgAGCGCGCGGGCGTGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGACCAGGCCATGGcc > 1:119069/2‑149 (MQ=255) aGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAtggcctggccag < 7:178392/149‑3 (MQ=255) gCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATGGCCg > 2:169941/1‑147 (MQ=255) cgcgggcgtgcTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATGGCCGCAGAc < 2:119069/144‑1 (MQ=255) cGCCCGCGCGCTCGAT‑CCGATTGCCGATAGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATGGCCGCAGa > 1:193045/1‑149 (MQ=255) cGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATGGCCGCAGAc < 1:169941/149‑1 (MQ=255) cgcgcgCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCAcc < 4:151293/47‑1 (MQ=255) | ATTTACCTGGGCGACCACGTGCGGGTGCGTCTGGAGGTTTGCGGCAAAGGCGACTTCTTCGTGAAGCAGCCGATTGCCGAGCTCGACCCGGCGTTGGCCGTGGGCGATGTAGTACCGCTGGGCTGGGAGGTGGAGCACGCCCGCGCGCTCGAT‑CCGATTGCCGA‑AGCCCATTGATGGATTGCTTCACCAACCCTGTACTGTGGAGAGAATAATAATGCGCAAGCAGTTGAAACTGACCGCCCTGGCTTTGGGGCTGTTCACCGCTGGCCAGGCCATGGCCGCAGAC > NC_002947/500403‑500688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |