Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,388,883	T→G	5.6%	L798R (CTG→CGG)	dld2 →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,388,883	0	T	G	5.6%	217.9 / 3.4	73	L798R (CTG→CGG)	dld2	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (34/34);  new base G (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

CTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCG  >  NC_002947/5388743‑5389026                                                                                                                                             |                                                                                                                                                ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAg                                                                                                                                          <  3:152544/148‑1 (MQ=255)   gcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGAc                                                                                                                                       >  3:34319/1‑149 (MQ=255)     ggCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCt                                                                                                                                     >  4:284882/1‑149 (MQ=255)       ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCt                                                                                                                                     >  5:152906/1‑147 (MQ=255)        cAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAg                                                                                                                                  <  4:34319/149‑1 (MQ=255)         aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGc                                                                                                                                 >  5:341294/1‑149 (MQ=255)              ttCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCt                                                                                                                                                 <  7:172541/128‑1 (MQ=255)              ttCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCt                                                                                                                                                 >  8:172541/1‑128 (MQ=255)               tCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACAcc                                                                                                                           >  6:142342/1‑149 (MQ=255)                  ccTCCAAGGGCTCCCCCGAGCCGGCCGAACACAAACGCCCGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGt                                                                                                                                              <  4:286837/127‑1 (MQ=255)                  ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGt                                                                                                                                              >  3:286837/1‑127 (MQ=255)                   cTCCAAGGGCTACCCCGAGCAGGCCGCACACAAACGCCAGGAGCTGATCCCCGCCCTGCTGCACGCCCGCCGCGGCGGCCTCGCCCCGCTCGATTGCGCCACCAGCCCCTGCCCCCTGCGCCTGGTGCAGGACCTGCGCGCCACCCGGc                                                                                                                       >  3:56135/1‑149 (MQ=255)                   cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                       >  3:165183/1‑149 (MQ=255)                      cacgggCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                     <  4:56135/145‑1 (MQ=255)                       aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGTGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                   <  1:223756/149‑1 (MQ=255)                       aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                   >  6:61427/1‑149 (MQ=255)                            cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgggg                                                                                                                                       <  6:64071/124‑3 (MQ=255)                            cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgggg                                                                                                                                       >  5:64071/1‑122 (MQ=255)                            cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgggg                                                                                                                                       <  6:205000/124‑3 (MQ=255)                            cTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCAGGGTGCAgggg                                                                                                                                       >  5:205000/1‑122 (MQ=255)                               ccccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTAc                                                                                                            <  3:284882/148‑1 (MQ=255)                               ccccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTAc                                                                                                            <  6:300116/148‑1 (MQ=255)                                 ccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAc                                                                                                         <  6:341294/149‑1 (MQ=255)                                  cGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGt                                                                                                                                              <  3:246189/111‑1 (MQ=255)                                  cGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGt                                                                                                                                              >  4:246189/1‑111 (MQ=255)                                       aGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCAGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                   >  4:313744/1‑149 (MQ=255)                                        ggCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgc                                                                                                  <  5:61427/149‑1 (MQ=255)                                         gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcg                                                                                                 >  7:170333/1‑149 (MQ=255)                                           cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGa                                                                                                                                   <  1:63389/113‑1 (MQ=255)                                           cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                   >  7:157385/1‑129 (MQ=255)                                           cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                   <  8:157385/129‑1 (MQ=255)                                           cGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCAGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGAGCCTGGTGCAGGACCTGa                                                                                                                                   >  2:63389/1‑113 (MQ=255)                                                acaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                          >  3:295131/1‑149 (MQ=255)                                                  aaaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCGGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                        >  5:198648/1‑149 (MQ=255)                                                       ccAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGGGCGCTTCATCCGcaccc                                                                                   <  6:276195/149‑1 (MQ=255)                                                                gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTc                                                                           >  5:258220/1‑148 (MQ=255)                                                                 aTCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTc                                                                           <  5:142342/147‑1 (MQ=255)                                                                    aCCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgacc                                                                       <  6:152906/148‑1 (MQ=255)                                                                     ccGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgaccga                                                                     >  2:230650/1‑149 (MQ=255)                                                                       gCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTg                                                                                                                                             <  5:308622/75‑1 (MQ=255)                                                                       gCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTg                                                                                                                                             >  6:308622/1‑75 (MQ=255)                                                                          ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCGTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                <  8:170333/149‑1 (MQ=255)                                                                          ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCGGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                                <  6:198648/149‑1 (MQ=255)                                                                             ctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGt                                                              <  1:230650/148‑1 (MQ=255)                                                                                 aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                         >  4:325162/1‑149 (MQ=255)                                                                                 aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGAAACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                         >  4:179885/1‑149 (MQ=255)                                                                                  cGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAccc                                                        >  4:92935/1‑149 (MQ=255)                                                                                     cAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgac                                                                        <  8:290819/130‑1 (MQ=255)                                                                                     cAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgac                                                                        >  7:290819/1‑130 (MQ=255)                                                                                      aGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCa                                                    <  4:295131/149‑1 (MQ=255)                                                                                        ccGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAgg                                                  >  5:101845/1‑149 (MQ=255)                                                                                             gcggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGaa                                             <  4:165183/149‑1 (MQ=255)                                                                                                  cTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                         >  3:104212/1‑148 (MQ=255)                                                                                                       cccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGt                                                              <  1:185738/122‑1 (MQ=255)                                                                                                       cccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGt                                                              >  2:185738/1‑122 (MQ=255)                                                                                                        ccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                                  <  3:325162/149‑1 (MQ=255)                                                                                                          gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGt                                        >  6:355714/1‑141 (MQ=255)                                                                                                          gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGt                                        <  5:355714/141‑1 (MQ=255)                                                                                                          gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                                 >  7:198257/1‑148 (MQ=255)                                                                                                          gATCTATTGCGACAAAAGCCCCTCCACCCTGCGCCTGGAGAAGGACCAGAGCGACACCCGCCTGGACCTGTACGACCCCCTGCGCTTCATCCGCACCCACCAGCTCGACCGACTCGAGTTAACCCCCCAGGACGAACCGCTAGCtgtg                                 >  8:82784/1‑148 (MQ=255)                                                                                                               aTTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                            >  6:76811/1‑148 (MQ=255)                                                                                                               aTTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTg                           >  7:129739/1‑149 (MQ=255)                                                                                                                ttGCGACACAAGCCCCTGCACGCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACGTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                            <  3:313744/147‑1 (MQ=255)                                                                                                                ttGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGc                                <  8:198257/143‑1 (MQ=255)                                                                                                                  gCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAcc                        >  5:26345/1‑149 (MQ=255)                                                                                                                      cacaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgca                    <  6:26345/149‑1 (MQ=255)                                                                                                                       acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                  <  2:126832/54‑1 (MQ=255)                                                                                                                       acaAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                  >  1:126832/1‑54 (MQ=255)                                                                                                                            ccccTGCACGCTGCGCCTGGTTCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcaccc              <  7:82784/149‑1 (MQ=255)                                                                                                                                 aggtccTGCACCAGGCGAATGACCTGAGCGAAAAACGGCTGGACCTGTACGAACACGTGCGCTTCATCATAACACACATGCTCGACCGACTGGAGTTAACCCACCAGGACGAACCGGTAGCTGTGCACGTTAACTGcagaacccagcac         >  6:70177/5‑149 (MQ=255)                                                                                                                                  cACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagcacc        >  6:106840/1‑149 (MQ=255)                                                                                                                                  cACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacccagcac         <  8:129739/148‑1 (MQ=255)                                                                                                                                     ccTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTgg     <  6:101845/149‑1 (MQ=255)                                                                                                                                         cgcCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGc   <  3:92935/147‑1 (MQ=255)                                                                                                                                         cgcCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCg  <  6:258220/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                CTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGCG  >  NC_002947/5388743‑5389026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑