Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,413,267 T→C 8.1% V301V (GTA→GTG)  PP_4755 ← ferrichrome‑iron receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,413,2670TC 8.1% 135.3 / 2.7 49V301V (GTA→GTG) PP_4755ferrichrome‑iron receptor
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base C (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TCGGTCGGCGCCCAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGTTGGCACCCCGCA  >  NC_002947/5413133‑5413412
                                                                                                                                      |                                                                                                                                                 
tcggtcggCGCCCAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGt                                                                                                                                     >  1:27971/1‑149 (MQ=255)
    tcggCGCCCAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGAGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCATACATCAATCCCGGTTTCg                                                                                                                                 >  7:245574/1‑149 (MQ=255)
    tcggCGCCCAGGTCACGCCGCCCATCACGAAGCGGCTATCGTCCTGCGACTCGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGGCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCg                                                                                                                                 <  6:284475/149‑1 (MQ=255)
           ccAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGttt                                                                                                                                   >  1:102589/1‑140 (MQ=255)
           ccAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGttt                                                                                                                                   <  2:102589/140‑1 (MQ=255)
           ccAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGt                                                                                                                          <  5:81420/149‑1 (MQ=255)
                         ccATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            >  3:52893/1‑149 (MQ=255)
                                gAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACg                                                                                                      >  1:166728/1‑148 (MQ=255)
                                   gCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGc                                                                                                  <  2:80508/149‑1 (MQ=255)
                                      gTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGt                                                                                                                                     <  4:128193/111‑1 (MQ=255)
                                      gTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGt                                                                                                                                     >  3:128193/1‑111 (MQ=255)
                                             tCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            <  1:128785/129‑1 (MQ=255)
                                             tCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            <  1:133888/129‑1 (MQ=255)
                                             tCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            >  2:128785/1‑129 (MQ=255)
                                             tCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            >  2:133888/1‑129 (MQ=255)
                                             tCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCAc                                                                                                       <  4:190901/134‑1 (MQ=255)
                                             tCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCAc                                                                                                       >  3:190901/1‑134 (MQ=255)
                                                        gTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAgc                                                                             >  4:266763/1‑149 (MQ=255)
                                                           gTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGcgcg                                                                          >  7:13456/1‑149 (MQ=255)
                                                               tCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            >  7:198409/1‑111 (MQ=255)
                                                               tCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAg                                                                                                            <  8:198409/111‑1 (MQ=255)
                                                                cgcgGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTtc                                                                                    <  3:314367/134‑1 (MQ=255)
                                                                cgcgGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTtc                                                                                    >  4:314367/1‑134 (MQ=255)
                                                                   ggTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTg                                                                  <  2:166728/149‑1 (MQ=255)
                                                                    gTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTgg                                                                 <  3:266763/149‑1 (MQ=255)
                                                                       gCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCa                                                              >  5:343127/1‑149 (MQ=255)
                                                                           ttCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACg                                                            >  1:327713/1‑147 (MQ=255)
                                                                           ttCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACg                                                            >  5:135324/1‑147 (MQ=255)
                                                                           ttCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGaa                                                          <  8:13456/149‑1 (MQ=255)
                                                                             cTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGt                                                        >  5:267179/1‑149 (MQ=255)
                                                                              tGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTa                                                                                                             >  3:170507/1‑95 (MQ=255)
                                                                              tGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTa                                                                                                             <  4:170507/95‑1 (MQ=255)
                                                                              tGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGtt                                                       >  5:182094/1‑149 (MQ=255)
                                                                                cATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTga                                                     <  6:135324/149‑1 (MQ=255)
                                                                                cATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTga                                                     <  2:327713/149‑1 (MQ=255)
                                                                                       gCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCCACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGcc                                                                                                 >  4:6199/1‑98 (MQ=255)
                                                                                       gCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCCACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGcc                                                                                                 <  3:1338/98‑1 (MQ=255)
                                                                                       gCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCCACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGcc                                                                                                 <  3:6199/98‑1 (MQ=255)
                                                                                       gCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATAGCCCACATCAATCCCGGTTTCGGCATTGTAATAGGAGCCGTAGTTCACTTAGcc                                                                                                 >  4:1338/1‑98 (MQ=255)
                                                                                                       ccAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCggg                              >  2:221092/1‑149 (MQ=255)
                                                                                                        cAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGc                             >  6:296930/1‑149 (MQ=255)
                                                                                                                 ttCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAgg                     <  6:182094/148‑1 (MQ=255)
                                                                                                                   cgtcgtTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTg                  <  2:27971/149‑1 (MQ=255)
                                                                                                                    gtcgtTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTg                  <  6:267179/148‑1 (MQ=255)
                                                                                                                            gcgcATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGTTGGCa         >  5:263100/1‑149 (MQ=255)
                                                                                                                               cATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGtt             <  1:19711/142‑1 (MQ=255)
                                                                                                                               cATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGtt             >  2:19711/1‑142 (MQ=255)
                                                                                                                                   gCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGTTGGCACCCCg    >  8:76156/1‑147 (MQ=255)
                                                                                                                                   gCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGTTGGCACCCCGCa  <  5:296930/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                 
TCGGTCGGCGCCCAGGTCAGGCCGCCCATCACGAAGCGGTTATCGTCCTGCGAATGGTCGTACTCGCGGTCGCTGTTCTGCATCTTGGCCGTGAAGCGGCCGGCCAGGGTTTGTTCGTCGTTCAGCGCATCGCCTACATCAATCCCGGTTTCGGCATGGTCATAGGAGCCGTAGGTCACGTAGCCTTGGCCAAACTTCTCGAAGCGCGGTTGCTTGGTCACGAAGTTGACCGACCCACCCGGGTCTGCCGGGCCGAACAGGGTGGAGTTGGCACCCCGCA  >  NC_002947/5413133‑5413412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑