Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,036 T→C 7.8% I95V (ATC→GTC)  PP_4769 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,0360TC 7.8% 141.5 / 2.2 51I95V (ATC→GTC) PP_4769hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGCCACGATCGAT  >  NC_002947/5429889‑5430177
                                                                                                                                                    |                                                                                                                                             
caCGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATc                                                                                                                                              <  5:73724/149‑1 (MQ=255)
  cGGATCAGTGACAACGCCGTCAGTCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGACATCACCGTCGCCGGAGGCCAGCACCAC‑CTGATCGACGCGGCCAGCCGGGTCGACcag                                                                                                                                            <  1:59755/149‑2 (MQ=255)
       cAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAATCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATc                                                                                                                                         >  7:201655/1‑147 (MQ=255)
        aGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGATCGACGCGGCCAGCCGGGTCGACcaggtggtg                                                                                                                                      >  4:217551/1‑142 (MQ=38)
         gTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAgg                                                                                                                                     <  6:338087/149‑1 (MQ=255)
          tGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAggg                                                                                                                                    >  1:319311/1‑149 (MQ=255)
            aCAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGt                                                                                                                                   <  4:321411/148‑1 (MQ=255)
               aCGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATg                                                                                                                               >  3:201681/1‑149 (MQ=255)
                 gCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGcc                                                                                                                             >  4:275349/1‑149 (MQ=255)
                                     aTACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTgg                                                                                                         <  5:36013/149‑1 (MQ=255)
                                     aTACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTgg                                                                                                         <  8:278933/149‑1 (MQ=255)
                                        caccacCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGcc                                                                                                       >  6:175215/1‑148 (MQ=255)
                                         accaccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGcc                                                                                                       >  6:337670/1‑147 (MQ=255)
                                           cacgGCTTCGGTGCCATGGCTCTGGTTTACCGGCTCCAGCAGCAAGTCGAAATCAACGTCGCCGTAGTCCAGCACCAG‑CTGGTCGAGCCGGCTGGCCGCGTCGATCACATCCAggg                                                                                                                                    <  8:155071/116‑1 (MQ=255)
                                           caccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGc                                                                                                   >  8:187086/1‑149 (MQ=255)
                                           caccGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGc                                                                                                   >  8:192145/1‑149 (MQ=255)
                                                cTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCa                                                                                              <  5:175215/149‑1 (MQ=255)
                                                         cATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGa                                                                                                     >  2:50263/1‑133 (MQ=255)
                                                               gcTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAAc                                                                               >  7:32698/1‑149 (MQ=255)
                                                                 tGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAg                                                                                                    >  5:215159/1‑126 (MQ=255)
                                                                 tGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAg                                                                                                    <  6:215159/126‑1 (MQ=255)
                                                                   gATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGtt                                                                           <  7:192145/149‑1 (MQ=255)
                                                                   gATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGtt                                                                           <  7:187086/149‑1 (MQ=255)
                                                                     ttACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGtt                                                                           <  6:289038/147‑1 (MQ=255)
                                                                     ttACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGtt                                                                           >  5:289038/1‑147 (MQ=255)
                                                                                           gAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTgcg                                                                                         <  2:81867/111‑1 (MQ=255)
                                                                                           gAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTgcg                                                                                         >  1:81867/1‑111 (MQ=255)
                                                                                           gAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGt                                                   >  4:162169/1‑149 (MQ=255)
                                                                                            aaaTCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGtt                                                  >  5:165416/1‑149 (MQ=255)
                                                                                              aTCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTgc                                                <  3:275349/149‑1 (MQ=255)
                                                                                               tCACCGTCGCCGGAGGCCAGCACCACGCTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGgc                                                                     >  1:4767/1‑128 (MQ=255)
                                                                                               tCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGgc                                                                     <  2:4767/127‑1 (MQ=255)
                                                                                                 aCCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTgcgc                                              <  6:165416/148‑1 (MQ=255)
                                                                                                    gTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTgcgc                                              >  2:114318/1‑145 (MQ=255)
                                                                                                        ccGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGa                                                                        >  4:347244/1‑115 (MQ=255)
                                                                                                        ccGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGa                                                                        <  3:347244/115‑1 (MQ=255)
                                                                                                        ccGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATctg                                      <  8:32698/149‑1 (MQ=255)
                                                                                                              gCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGaa                                <  2:319311/149‑1 (MQ=255)
                                                                                                                cAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCaa                                            <  6:342727/135‑1 (MQ=255)
                                                                                                                   caccac‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAActgct                           <  4:201681/149‑1 (MQ=255)
                                                                                                                      cac‑cTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCtgtt                        <  5:337670/149‑1 (MQ=255)
                                                                                                                             gTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAACCCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGc                  >  3:217172/1‑149 (MQ=255)
                                                                                                                             gTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGc                  >  6:110688/1‑149 (MQ=255)
                                                                                                                             atCGACGCGGCCAGCCGGGTCGACCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGc                  >  3:115806/2‑149 (MQ=255)
                                                                                                                               cGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTg                <  5:110688/149‑1 (MQ=255)
                                                                                                                                 aCCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTc              >  6:235027/1‑149 (MQ=255)
                                                                                                                                   gcggccagCCGGGTCGACCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGc            <  4:115806/142‑1 (MQ=255)
                                                                                                                                    cGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCACACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGc            >  3:338036/1‑148 (MQ=255)
                                                                                                                                             gcgTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAGCCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTg                   <  3:266548/132‑1 (MQ=255)
                                                                                                                                             gcgTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAGCCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTg                   >  4:266548/1‑132 (MQ=255)
                                                                                                                                             gcgTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGCCAcgatcgat  >  6:58602/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CACGGATCAGTGACAACGCCGTCAGCCCCGGCACGCCATACACCACCGCTTCGGTGCCATGGCGCTGGATTACCCGCTCCAGCAGCAAGTCGAAATCACCGTCGCCGGAGGCCAGCACCAC‑CTGGTCGACCCGGCTGGCCGCGTCGATCACATCCAGGGTAATGCCCACATCCCAGTCACCTTTGGCCGAGCCATCACTGCGCTGGATGAACGGTTTGAGGCGCACCTCGAAACCGAGGTTGCGCAAGATCTGCTGGAACTGCTGTTGTTTGCTGTCGCCACGATCGAT  >  NC_002947/5429889‑5430177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑