Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,392 A→G 7.8% intergenic (‑74/+87) PP_4769 ← / ← PP_4770 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,3920AG 7.8% 181.5 / 4.2 64intergenic (‑74/+87)PP_4769/PP_4770hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base G (2/3);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

AAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCG  >  NC_002947/5430245‑5430539
                                                                                                                                                   |                                                                                                                                                      
aaaTGGCAGGCGTGGGCCTGGCGCACCGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAg                                                                                                                                                       <  3:30687/149‑1 (MQ=255)
  tactacaccGTGCGCCAGGCCCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAg                                                                                                                                                       <  6:302650/140‑1 (MQ=255)
   tGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTa                                                                                                                                                    >  3:31526/1‑149 (MQ=255)
        accGTGCGCCAGGCCCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcg                                                                                                                                              >  4:285156/2‑149 (MQ=255)
                      gcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                                               >  8:217797/1‑134 (MQ=255)
                      gcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                                               <  7:217797/134‑1 (MQ=255)
                      gcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTggg                                                                                                                                >  3:79080/1‑149 (MQ=255)
                        aCGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGC‑GCTTTGCGCCCCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGc                                                                                                                             <  4:297557/149‑1 (MQ=18)
                           gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                           <  3:285156/149‑1 (MQ=255)
                           gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                           <  8:148424/149‑1 (MQ=255)
                              tagtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCGCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGTGAGCGGGc                                                                                                                         <  6:10824/148‑1 (MQ=255)
                                  agagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCACTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGcc                                                                                                                    >  5:120145/1‑149 (MQ=255)
                                   gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGccc                                                                                                                   >  7:146005/1‑149 (MQ=255)
                                           gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                 >  5:297716/1‑127 (MQ=255)
                                           gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                 <  6:297716/127‑1 (MQ=255)
                                           gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGGGCGGGCGTGCCCGCGAAgg‑‑g                                                                                                         >  5:174730/1‑149 (MQ=255)
                                            cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgcg                                                                                                                                            <  6:276926/115‑1 (MQ=255)
                                            cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg‑‑g                                                                                                         <  4:79080/148‑1 (MQ=255)
                                            cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg‑‑g                                                                                                         <  4:31526/148‑1 (MQ=255)
                                             acaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑Ggc                                                                                                       <  7:262132/149‑1 (MQ=255)
                                               aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAggg                                                                                                            <  6:340882/144‑1 (MQ=255)
                                               aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAggg                                                                                                            >  5:340882/1‑144 (MQ=255)
                                               aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑Ggcgc                                                                                                     <  8:146005/149‑1 (MQ=255)
                                                     gAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAgcg                                                                                               >  5:18175/1‑149 (MQ=255)
                                                         aGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                 >  7:67062/1‑113 (MQ=255)
                                                         aGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                 <  8:67062/113‑1 (MQ=255)
                                                              gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGa                                                                                       >  3:289410/1‑148 (MQ=255)
                                                              gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGa                                                                                       >  2:277586/1‑148 (MQ=255)
                                                              gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGa                                                                                       >  8:64855/1‑148 (MQ=255)
                                                                 cTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAAt                                                                                    <  6:18175/148‑1 (MQ=255)
                                                                  tttttCACCTGGGAACCTCATTTTTTGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                  >  5:207604/1‑149 (MQ=255)
                                                                    tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGGGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                  <  4:47953/147‑1 (MQ=255)
                                                                    tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGATAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGccccctcgcgg                                                                                    <  1:304566/145‑7 (MQ=255)
                                                                        tcgtGGGAACCTAATTTTTGGCGTGGTGTGCCGGGGGGGTGCTGCGCATACCTGCGGGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCGGTGGGTGGGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGcccc                                                                                           <  8:281012/131‑1 (MQ=255)
                                                                           tGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa                                                                          >  2:49497/1‑148 (MQ=255)
                                                                            gggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATg                                                                        >  8:267440/1‑149 (MQ=255)
                                                                             ggAACCTCATTTTTTGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAt                                                                         <  6:207604/147‑1 (MQ=255)
                                                                             ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa                                                                          <  1:49497/146‑1 (MQ=255)
                                                                             ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGc                                                                       <  4:289410/149‑1 (MQ=255)
                                                                                 ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGa                                                                   <  7:64855/149‑1 (MQ=255)
                                                                                  cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAAt                                                                                    <  6:2070/131‑1 (MQ=255)
                                                                                   tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACCCCCCCCCACCCAAAGGGAT‑AGCGCGTAACCCCGGGGGCGCGGGCGTGCCCGCCGAGG‑‑GGGGCAAAGCCCCCCCCGAAAAGACAGCCCTATTCCacccc                                                                 >  6:314618/1‑145 (MQ=255)
                                                                                   tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc                                                                 >  5:117672/1‑149 (MQ=255)
                                                                                            gCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGAc                                                         <  7:267440/148‑1 (MQ=255)
                                                                                               tGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGTGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa                                                     >  8:274357/1‑149 (MQ=255)
                                                                                                    tGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtc                                                <  1:277586/149‑1 (MQ=255)
                                                                                                      ccGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcg                                               <  6:117672/148‑1 (MQ=255)
                                                                                                       cGGGGGGCTTCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTTT‑CGCGCTTACACCCGTGGGAGCGGGCGTGCCCGCGAATG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCATTATGACAGACCTGTCAg                                                           <  2:80085/135‑1 (MQ=38)
                                                                                                       cGGGGGGCTGCTGCGCAGACCTTCGCCGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCGGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAg                                                           >  1:80085/1‑135 (MQ=255)
                                                                                                                  tgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGagaag                                   >  7:87362/1‑148 (MQ=255)
                                                                                                                      cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                  <  8:54402/97‑1 (MQ=255)
                                                                                                                      cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                  >  7:54402/1‑97 (MQ=255)
                                                                                                                             tCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                             <  6:219082/127‑1 (MQ=255)
                                                                                                                             tCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                             >  5:219082/1‑127 (MQ=255)
                                                                                                                               gcgGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATc                     <  5:314618/149‑1 (MQ=255)
                                                                                                                                     acGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                >  4:250973/1‑148 (MQ=255)
                                                                                                                                       gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTc                                                             >  6:364354/1‑101 (MQ=255)
                                                                                                                                       gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTc                                                             <  5:364354/101‑1 (MQ=255)
                                                                                                                                         ccGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGt                                                       <  2:11767/105‑1 (MQ=18)
                                                                                                                                            cTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGGTCTGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac          >  3:231541/1‑149 (MQ=21)
                                                                                                                                             tCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa           <  7:293466/145‑1 (MQ=37)
                                                                                                                                             tCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa           >  8:293466/1‑145 (MQ=37)
                                                                                                                                             tCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCgag                                      >  3:65911/1‑118 (MQ=255)
                                                                                                                                             tCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCgag                                      <  4:65911/118‑1 (MQ=255)
                                                                                                                                                  caGGTAT‑CGCGCGTACACCCGTGGGTGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCg  <  6:174730/149‑1 (MQ=255)
                                                                                                                                                  caGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCg  <  3:250973/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
AAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCG  >  NC_002947/5430245‑5430539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑