Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,410 C→T 9.4% intergenic (‑92/+69) PP_4769 ← / ← PP_4770 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,4100CT 9.4% 172.7 / 5.6 64intergenic (‑92/+69)PP_4769/PP_4770hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/31);  new base T (2/4);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

GCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCA  >  NC_002947/5430267‑5430557
                                                                                                                                                |                                                                                                                                                     
gcACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTggg                                                                                                                                                  >  3:79080/1‑149 (MQ=255)
  aCGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGC‑GCTTTGCGCCCCTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGc                                                                                                                                               <  4:297557/149‑1 (MQ=18)
     gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                                             <  8:148424/149‑1 (MQ=255)
     gtgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                                             <  3:285156/149‑1 (MQ=255)
        tagtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCGCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGTGAGCGGGc                                                                                                                                           <  6:10824/148‑1 (MQ=255)
            agagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCACTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGcc                                                                                                                                      >  5:120145/1‑149 (MQ=255)
             gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGccc                                                                                                                                     >  7:146005/1‑149 (MQ=255)
                     gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                                   <  6:297716/127‑1 (MQ=255)
                     gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                                   >  5:297716/1‑127 (MQ=255)
                     gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGGGCGGGCGTGCCCGCGAAgg‑‑g                                                                                                                           >  5:174730/1‑149 (MQ=255)
                      cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg‑‑g                                                                                                                           <  4:79080/148‑1 (MQ=255)
                      cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg‑‑g                                                                                                                           <  4:31526/148‑1 (MQ=255)
                       acaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑Ggc                                                                                                                         <  7:262132/149‑1 (MQ=255)
                         aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAggg                                                                                                                              <  6:340882/144‑1 (MQ=255)
                         aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAggg                                                                                                                              >  5:340882/1‑144 (MQ=255)
                         aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑Ggcgc                                                                                                                       <  8:146005/149‑1 (MQ=255)
                               gAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAgcg                                                                                                                 >  5:18175/1‑149 (MQ=255)
                                   aGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                                   >  7:67062/1‑113 (MQ=255)
                                   aGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                                   <  8:67062/113‑1 (MQ=255)
                                        gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGa                                                                                                         >  2:277586/1‑148 (MQ=255)
                                        gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGa                                                                                                         >  8:64855/1‑148 (MQ=255)
                                        gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGa                                                                                                         >  3:289410/1‑148 (MQ=255)
                                           cTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAAt                                                                                                      <  6:18175/148‑1 (MQ=255)
                                            tttttCACCTGGGAACCTCATTTTTTGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                                    >  5:207604/1‑149 (MQ=255)
                                              tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGGGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                                    <  4:47953/147‑1 (MQ=255)
                                              tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGATAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGccccctcgcgg                                                                                                      <  1:304566/145‑7 (MQ=255)
                                                  tcgtGGGAACCTAATTTTTGGCGTGGTGTGCCGGGGGGGTGCTGCGCATACCTGCGGGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCGGTGGGTGGGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGcccc                                                                                                             <  8:281012/131‑1 (MQ=255)
                                                     tGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa                                                                                            >  2:49497/1‑148 (MQ=255)
                                                      gggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATg                                                                                          >  8:267440/1‑149 (MQ=255)
                                                       ggAACCTCATTTTTTGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTAt                                                                                           <  6:207604/147‑1 (MQ=255)
                                                       ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTa                                                                                            <  1:49497/146‑1 (MQ=255)
                                                       ggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGc                                                                                         <  4:289410/149‑1 (MQ=255)
                                                           ccTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGa                                                                                     <  7:64855/149‑1 (MQ=255)
                                                            cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAAt                                                                                                      <  6:2070/131‑1 (MQ=255)
                                                             tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACCCCCCCCCACCCAAAGGGAT‑AGCGCGTAACCCCGGGGGCGCGGGCGTGCCCGCCGAGG‑‑GGGGCAAAGCCCCCCCCGAAAAGACAGCCCTATTCCacccc                                                                                   >  6:314618/1‑145 (MQ=255)
                                                             tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc                                                                                   >  5:117672/1‑149 (MQ=255)
                                                                      gCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGAc                                                                           <  7:267440/148‑1 (MQ=255)
                                                                         tGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGTGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa                                                                       >  8:274357/1‑149 (MQ=255)
                                                                              tGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtc                                                                  <  1:277586/149‑1 (MQ=255)
                                                                                ccGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcg                                                                 <  6:117672/148‑1 (MQ=255)
                                                                                 cGGGGGGCTTCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTTT‑CGCGCTTACACCCGTGGGAGCGGGCGTGCCCGCGAATG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCATTATGACAGACCTGTCAg                                                                             <  2:80085/135‑1 (MQ=38)
                                                                                 cGGGGGGCTGCTGCGCAGACCTTCGCCGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCGGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAg                                                                             >  1:80085/1‑135 (MQ=255)
                                                                                            tgcGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGagaag                                                     >  7:87362/1‑148 (MQ=255)
                                                                                                cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                                    <  8:54402/97‑1 (MQ=255)
                                                                                                cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGc                                                                                                    >  7:54402/1‑97 (MQ=255)
                                                                                                       tCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                                               <  6:219082/127‑1 (MQ=255)
                                                                                                       tCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtc                                                               >  5:219082/1‑127 (MQ=255)
                                                                                                         gcgGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATc                                       <  5:314618/149‑1 (MQ=255)
                                                                                                               acGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGc                                  >  4:250973/1‑148 (MQ=255)
                                                                                                                 gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTc                                                                               <  5:364354/101‑1 (MQ=255)
                                                                                                                 gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTc                                                                               >  6:364354/1‑101 (MQ=255)
                                                                                                                   ccGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGt                                                                         <  2:11767/105‑1 (MQ=18)
                                                                                                                      cTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGGTCTGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac                            >  3:231541/1‑149 (MQ=21)
                                                                                                                       tCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa                             >  8:293466/1‑145 (MQ=37)
                                                                                                                       tCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa                             <  7:293466/145‑1 (MQ=37)
                                                                                                                       tCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCgag                                                        <  4:65911/118‑1 (MQ=255)
                                                                                                                       tCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCgag                                                        >  3:65911/1‑118 (MQ=255)
                                                                                                                            caGGTAT‑CGCGCGTACACCCGTGGGTGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCg                    <  6:174730/149‑1 (MQ=255)
                                                                                                                            caGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCg                    <  3:250973/149‑1 (MQ=255)
                                                                                                                                  t‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa                             >  5:11684/1‑134 (MQ=255)
                                                                                                                                  t‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaaa                             <  6:11684/134‑1 (MQ=255)
                                                                                                                                       cgcgatacCTGTGGGAGCGGGCGTGTCCGCGAAGGTCTGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATcc            <  4:231541/143‑1 (MQ=38)
                                                                                                                                            cacCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGTGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTgg     <  7:274357/149‑1 (MQ=255)
                                                                                                                                             acCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTgg     >  3:36068/1‑148 (MQ=255)
                                                                                                                                               ccGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac                            <  5:225282/123‑1 (MQ=255)
                                                                                                                                               ccGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac                            >  6:225282/1‑123 (MQ=255)
                                                                                                                                               ccGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCt       <  6:62033/144‑1 (MQ=255)
                                                                                                                                               ccGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCt       >  5:62033/1‑144 (MQ=255)
                                                                                                                                               ccGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCACCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCa  >  5:26527/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGG‑‑GGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTATCCGCCCTGGTCA  >  NC_002947/5430267‑5430557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑