Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,473,817	G→A	9.8%	D326D (GAC→GAT)	proA ←	gamma‑glutamyl phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,473,817	0	G	A	9.8%	167.1 / 6.2	61	D326D (GAC→GAT)	proA	gamma‑glutamyl phosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base A (3/3);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GCAAAGCAGGTCGGGGTGTTGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAGCGGCGGGCCATTTCT  >  NC_002947/5473671‑5473961                                                                                                                                                   |                                                                                                                                                  gCAAAGCAGGTCGGGGTGTTGAGCATGACCGACGCCGAATAGACCTCAGCCATGAACTGACGGGCTTAACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGACGGCCTGAACCAGGCCATc                                                                                                                                                 >  5:215419/1‑149 (MQ=255)   aaaGCAGGTCGGGGTGTTGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGa                                                                                                                                               >  5:299751/1‑149 (MQ=255)   aaaGAAGGTAGGGGTGTTGATCATTACCGACGCCGAATCGACATCAGCCATGAACTGACCGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGACGGCCTGAACCAGGCCATCGa                                                                                                                                               <  6:215419/149‑1 (MQ=255)       cAGGGCGGGGTGTTGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCAc                                                                                                                                           <  5:260422/149‑1 (MQ=255)              gggTGTTGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATc                                                                                                                                    >  6:175603/1‑149 (MQ=255)                  gtTGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACa                                                                                                                                <  6:364280/149‑1 (MQ=255)                   ttGAGCATGCCCGACGCCGAATCGCCCTCAGCCATGAACGGACGGGCTTCCCCCTGGTGTGCGCTGCTGCTCGAGGCGGTGTGGGGCGAGCCATAGTGGTTGATGGGCGCGATGGCCTGGGTCAGGCCGGCGACCACGCGAATCGACAg                                                                                                                               <  6:270045/149‑1 (MQ=255)                   ttGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGGTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAg                                                                                                                               <  6:275015/149‑1 (MQ=255)                       gCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑c                                                                                                                          >  4:198938/1‑149 (MQ=255)                        cATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑Cg                                                                                                                         >  1:35988/1‑149 (MQ=255)                              cGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATc                                                                                                                     >  8:56692/1‑147 (MQ=255)                               gACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAg                                                                                                                  <  3:198938/149‑1 (MQ=255)                                     gAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGACGGCCTGAACCAGGCCATc                                                                                                                                                 >  6:185184/1‑113 (MQ=255)                                     gAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGACGGCCTGAACCAGGCCATc                                                                                                                                                 <  5:185184/113‑1 (MQ=255)                                              tCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGc                                                                                                     >  7:293543/1‑147 (MQ=255)                                                    aTGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGc                                                                                             >  3:259546/1‑149 (MQ=255)                                                     tGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCt                                                                                            >  2:232753/1‑149 (MQ=255)                                                           gACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTg                                                                                      >  8:135591/1‑149 (MQ=255)                                                            aCGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGATCCGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGt                                                                                       <  1:232753/148‑1 (MQ=255)                                                            aCGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTg                                                                                      <  4:259546/148‑1 (MQ=255)                                                            aCGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCCACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGt                                                                                       <  1:68068/147‑1 (MQ=255)                                                              gggCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGcc                                                                                   <  7:135591/149‑1 (MQ=255)                                                                     aCCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTgg                                                                            >  5:33216/1‑149 (MQ=255)                                                                       ccTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGcc                                                                                   <  2:203068/140‑1 (MQ=255)                                                                       ccTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGgcg                                                                          >  1:205396/1‑149 (MQ=255)                                                                       ccTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGACCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGgcg                                                                          >  1:274987/1‑149 (MQ=255)                                                                              ttCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtgatga                                                                   <  2:274987/149‑1 (MQ=255)                                                                                  cTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCg                                                                                  <  4:128390/130‑1 (MQ=255)                                                                                  cTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCg                                                                                  >  3:128390/1‑130 (MQ=255)                                                                                     atgatCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTg                                                            <  7:56692/149‑1 (MQ=255)                                                                                     atgatCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTg                                                            >  3:242728/1‑149 (MQ=255)                                                                                      tgatCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTgg                                                           <  1:178090/149‑1 (MQ=255)                                                                                      tgatCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTgg                                                           <  2:35988/149‑1 (MQ=255)                                                                                       gatCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTggg                                                          >  3:2361/1‑149 (MQ=255)                                                                                       gatCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGGGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTgg                                                           <  5:360207/148‑1 (MQ=255)                                                                                                        gCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGT‑GCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACcgcg                                          >  5:56544/1‑147 (MQ=255)                                                                                                          gAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAg                                       >  1:2160/1‑149 (MQ=255)                                                                                                             ccATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTc                                    <  8:293543/149‑1 (MQ=255)                                                                                                              cATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTc                                    >  7:281991/1‑148 (MQ=255)                                                                                                                tAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCAc                                 >  4:345001/1‑149 (MQ=255)                                                                                                                  gTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGc                               >  8:29342/1‑149 (MQ=255)                                                                                                                     gTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCt                                                             <  8:325757/116‑1 (MQ=255)                                                                                                                     gTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCt                                                             >  7:325757/1‑116 (MQ=255)                                                                                                                     gTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGccc                             <  8:281991/148‑1 (MQ=255)                                                                                                                         aTGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTtctc                        <  5:175603/149‑1 (MQ=255)                                                                                                                             ggtCGACGGCCTGAACCAGGCCATCGAGCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtgat                                                                     <  7:65671/98‑1 (MQ=37)                                                                                                                             ggtCGACGGCCTGAACCAGGCCATCGAGCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtgat                                                                     >  8:65671/3‑100 (MQ=37)                                                                                                                             gCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCgtgt                                                                                                       <  6:120939/66‑1 (MQ=255)                                                                                                                             gCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCgtgt                                                                                                       >  5:120939/1‑66 (MQ=255)                                                                                                                             gCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCAc                     >  6:339572/1‑148 (MQ=255)                                                                                                                                cGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAg                 >  2:310869/1‑149 (MQ=255)                                                                                                                                 gATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtgat                                                                     <  4:11827/96‑1 (MQ=255)                                                                                                                                 gATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtgat                                                                     >  3:11827/1‑96 (MQ=255)                                                                                                                                 gATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAgc                >  3:206368/1‑149 (MQ=255)                                                                                                                                  aTGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAgcg               >  3:32506/1‑149 (MQ=255)                                                                                                                                     gCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAgcggc             <  2:205396/148‑1 (MQ=255)                                                                                                                                       cTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAGCGGCggg          <  4:206368/149‑1 (MQ=255)                                                                                                                                           ttCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAGCGGCGGGCCAt      <  2:2160/149‑1 (MQ=255)                                                                                                                                               ggCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAGCGGCGGGCCATTTCt  <  4:2361/149‑1 (MQ=255)                                                                                                                                                  cGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtga                                                                      >  7:62211/1‑78 (MQ=255)                                                                                                                                                  cGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCtga                                                                      <  8:62211/78‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GCAAAGCAGGTCGGGGTGTTGAGCATGACCGACGCCGAATCGACCTCAGCCATGAACTGACGGGCTTCACCCTGGTGTTCGCTGATGATCGAGTCGGTGTGGTGCGAGCCATAGTGGTTGATGTGCTCGATGGCCTGGTTCAGGCCGTCGACCACGCGAATCGACAGGAT‑CGCATCGAGGTATTCCGTGTGCCAGTCGGCTTCGGTGGCCGGTTTGGCGCTGATGATAGCCTGGGTACGCTCGCAACCGCGCAGCTCCACGCCCTTCTCCACGAAGCGGCGGGCCATTTCT  >  NC_002947/5473671‑5473961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑