Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,483,191	G→T	7.7%	R36L (CGC→CTC)	PP_4821 →	Fis‑like DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,483,191	0	G	T	7.7%	202.4 / 6.4	65	R36L (CGC→CTC)	PP_4821	Fis‑like DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (32/28);  new base T (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

ACAGAGCCTTGAGACAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGAC  >  NC_002947/5483046‑5483332                                                                                                                                                  |                                                                                                                                                  aCAGAGCCTTGAGACAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGa                                                                                                                                                >  7:213252/1‑149 (MQ=255)            agaCAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGTGACAACGCCATCCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCgag                                                                                                                                      <  7:141623/148‑1 (MQ=255)              aCAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAg                                                                                                                                   >  5:206237/1‑149 (MQ=255)                agagGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGgc                                                                                                                                 >  2:335894/1‑149 (MQ=255)                agagGACGGACAAGGGGTGGCAGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACTCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCCAGAGAAGAGGGCCAGACACGTCGCGACAGCGTCGAGAAGgc                                                                                                                                 >  4:306139/1‑149 (MQ=255)                   ggacggacAAGGGGGTGCCGCATGACGATTATGACCTAGAATTTTGTGATTGTAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑Ct                                                                                                                             <  8:137029/149‑1 (MQ=255)                    gacggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑g                                                                                                                          >  4:158014/1‑149 (MQ=255)                     acggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gc                                                                                                                         <  3:311368/149‑1 (MQ=255)                       ggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                  >  8:298682/1‑141 (MQ=255)                       ggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                  <  7:298682/141‑1 (MQ=255)                         acAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACaa                                                                                                                     <  5:331649/149‑1 (MQ=255)                                   gCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGcc                                                                                                           >  7:39753/1‑149 (MQ=255)                                     cGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCa                                                                                                         <  3:158014/149‑1 (MQ=255)                                      gCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCAc                                                                                                        >  7:87105/1‑149 (MQ=255)                                        aTGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCt                                                                                                      >  8:114514/1‑149 (MQ=255)                                                gatgaCCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGgcg                                                                                              >  7:74336/1‑149 (MQ=255)                                                     ccGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gcac                                                                                                                       >  6:229545/1‑119 (MQ=255)                                                     ccGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gcac                                                                                                                       <  5:229545/119‑1 (MQ=255)                                                      cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑g                                                                                                                          <  3:91370/115‑1 (MQ=255)                                                      cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑g                                                                                                                          >  4:91370/1‑115 (MQ=255)                                                      cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCg                                                                                        >  2:107507/1‑149 (MQ=255)                                                      cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCg                                                                                        >  2:282302/1‑149 (MQ=255)                                                       gagaATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGaa                                                                                                                                    >  6:215045/1‑107 (MQ=255)                                                        agaATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTg                                                                                      <  8:39753/149‑1 (MQ=255)                                                            ttttGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTgac‑g                                                                                  >  2:114263/1‑148 (MQ=255)                                                                    gTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTgac‑ggac                                                                               >  6:330177/1‑143 (MQ=255)                                                                    gTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTgac‑ggac                                                                               <  5:330177/143‑1 (MQ=255)                                                                         acaacGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGATCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                  >  6:142003/1‑91 (MQ=255)                                                                         acaacGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGATCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                  <  5:142003/91‑1 (MQ=255)                                                                          aaacGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCTGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTg                                                                   <  6:278528/148‑1 (MQ=255)                                                                             cGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTg                                                                <  8:213252/149‑1 (MQ=255)                                                                              gCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGc                                                               >  1:288882/1‑149 (MQ=255)                                                                                ccGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGctc                                                             >  6:346757/1‑149 (MQ=255)                                                                                   tGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTcc                                                          >  2:214364/1‑149 (MQ=255)                                                                                      gCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGaa                                                       <  1:214364/149‑1 (MQ=255)                                                                                      gCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGaa                                                       <  7:114514/149‑1 (MQ=255)                                                                                           aaCGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGa                                                  <  8:74336/149‑1 (MQ=255)                                                                                            acccaaaCCTTAAACAGCACCTCAACACGCCCACCTAATAGGGCAATACCCTTCGCTACTGCGTGTTTAAGGGGTCTTCCCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTc                                                    <  1:282302/144‑1 (MQ=255)                                                                                            aCGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAg                                                 <  8:87105/149‑1 (MQ=255)                                                                                                     tGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTg                                        >  6:198985/1‑149 (MQ=255)                                                                                                     tGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTg                                        >  4:141872/1‑149 (MQ=255)                                                                                                      gAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGc                                       >  2:78254/1‑149 (MQ=255)                                                                                                        aaCAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTc                                     <  5:198985/149‑1 (MQ=255)                                                                                                        aaCAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTc                                     <  2:288882/149‑1 (MQ=255)                                                                                                         aaaGCACCTCAACACGGCGTGCGAAGAGGGCCAGACCCTTAGCGACATCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGACTGGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTc                                     <  1:78254/147‑1 (MQ=255)                                                                                                         aCAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAATGCGCGACCGTGAC‑GGCCGTGTACAACCTGGTTCTCTCCGAAGTCGAGGCGCCCctcctc                                     >  8:226991/1‑148 (MQ=255)                                                                                                            gCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGaaa                                 <  1:107507/149‑1 (MQ=255)                                                                                                            gCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGaaa                                 <  3:141872/149‑1 (MQ=255)                                                                                                            gCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGaaa                                 <  5:346757/149‑1 (MQ=255)                                                                                                                 tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGgcgc                                             <  6:263026/132‑1 (MQ=255)                                                                                                                 tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGgcgc                                             >  5:263026/1‑132 (MQ=255)                                                                                                                 tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAg                                <  6:231091/145‑1 (MQ=255)                                                                                                                 tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAg                                >  5:231091/1‑145 (MQ=255)                                                                                                                        gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gc                                                                                                                         >  8:322257/1‑50 (MQ=255)                                                                                                                        gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gc                                                                                                                         <  7:322257/50‑1 (MQ=255)                                                                                                                        gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                           >  2:241451/1‑143 (MQ=255)                                                                                                                        gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                           <  1:241451/143‑1 (MQ=255)                                                                                                                         ccGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTa                    >  6:147736/1‑149 (MQ=255)                                                                                                                              cGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTg                >  4:210716/1‑148 (MQ=255)                                                                                                                                  gagGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAggg           >  3:4724/1‑149 (MQ=255)                                                                                                                                   agGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                           <  1:199337/132‑1 (MQ=255)                                                                                                                                   agGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                           >  2:199337/1‑132 (MQ=255)                                                                                                                                       ccAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGaa              <  6:2425/141‑1 (MQ=255)                                                                                                                                       ccAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAgagatcgg      >  5:2425/1‑142 (MQ=255)                                                                                                                                            gccTTCTCGACGCTGTCGCGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGAc  >  4:273510/2‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ACAGAGCCTTGAGACAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGAC  >  NC_002947/5483046‑5483332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑