Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,483,196 A→G 7.8% S38G (AGC→GGC) ‡ PP_4821 → Fis‑like DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,483,1960AG 7.8% 179.3 / 4.9 65S38G (AGC→GGC) ‡PP_4821Fis‑like DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (32/27);  new base G (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGACCAAGGC  >  NC_002947/5483057‑5483338
                                                                                                                                           |                                                                                                                                                  
agaCAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGTGACAACGCCATCCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCgag                                                                                                                                            <  7:141623/148‑1 (MQ=255)
  aCAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAg                                                                                                                                         >  5:206237/1‑149 (MQ=255)
    agagGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGgc                                                                                                                                       >  2:335894/1‑149 (MQ=255)
    agagGACGGACAAGGGGTGGCAGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACTCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCCAGAGAAGAGGGCCAGACACGTCGCGACAGCGTCGAGAAGgc                                                                                                                                       >  4:306139/1‑149 (MQ=255)
       ggacggacAAGGGGGTGCCGCATGACGATTATGACCTAGAATTTTGTGATTGTAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑Ct                                                                                                                                   <  8:137029/149‑1 (MQ=255)
        gacggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑g                                                                                                                                >  4:158014/1‑149 (MQ=255)
         acggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gc                                                                                                                               <  3:311368/149‑1 (MQ=255)
           ggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                        <  7:298682/141‑1 (MQ=255)
           ggacAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                        >  8:298682/1‑141 (MQ=255)
             acAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACaa                                                                                                                           <  5:331649/149‑1 (MQ=255)
                       gCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGcc                                                                                                                 >  7:39753/1‑149 (MQ=255)
                         cGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCa                                                                                                               <  3:158014/149‑1 (MQ=255)
                          gCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCAc                                                                                                              >  7:87105/1‑149 (MQ=255)
                            aTGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCt                                                                                                            >  8:114514/1‑149 (MQ=255)
                                    gatgaCCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGgcg                                                                                                    >  7:74336/1‑149 (MQ=255)
                                         ccGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gcac                                                                                                                             >  6:229545/1‑119 (MQ=255)
                                         ccGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gcac                                                                                                                             <  5:229545/119‑1 (MQ=255)
                                          cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑g                                                                                                                                >  4:91370/1‑115 (MQ=255)
                                          cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑g                                                                                                                                <  3:91370/115‑1 (MQ=255)
                                          cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCg                                                                                              >  2:282302/1‑149 (MQ=255)
                                          cGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCg                                                                                              >  2:107507/1‑149 (MQ=255)
                                           gagaATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGaa                                                                                                                                          >  6:215045/1‑107 (MQ=255)
                                            agaATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTg                                                                                            <  8:39753/149‑1 (MQ=255)
                                                ttttGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTgac‑g                                                                                        >  2:114263/1‑148 (MQ=255)
                                                        gTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTgac‑ggac                                                                                     <  5:330177/143‑1 (MQ=255)
                                                        gTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTgac‑ggac                                                                                     >  6:330177/1‑143 (MQ=255)
                                                             acaacGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGATCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                        >  6:142003/1‑91 (MQ=255)
                                                             acaacGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGATCCTTCTCGACGCTGTCGCGAAgg                                                                                                                                        <  5:142003/91‑1 (MQ=255)
                                                              aaacGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCTGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTg                                                                         <  6:278528/148‑1 (MQ=255)
                                                                 cGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTg                                                                      <  8:213252/149‑1 (MQ=255)
                                                                  gCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGc                                                                     >  1:288882/1‑149 (MQ=255)
                                                                    ccGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGctc                                                                   >  6:346757/1‑149 (MQ=255)
                                                                       tGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTcc                                                                >  2:214364/1‑149 (MQ=255)
                                                                          gCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGaa                                                             <  7:114514/149‑1 (MQ=255)
                                                                          gCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGaa                                                             <  1:214364/149‑1 (MQ=255)
                                                                               aaCGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGa                                                        <  8:74336/149‑1 (MQ=255)
                                                                                acccaaaCCTTAAACAGCACCTCAACACGCCCACCTAATAGGGCAATACCCTTCGCTACTGCGTGTTTAAGGGGTCTTCCCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTc                                                          <  1:282302/144‑1 (MQ=255)
                                                                                aCGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAg                                                       <  8:87105/149‑1 (MQ=255)
                                                                                         tGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTg                                              >  4:141872/1‑149 (MQ=255)
                                                                                         tGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTg                                              >  6:198985/1‑149 (MQ=255)
                                                                                          gAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGc                                             >  2:78254/1‑149 (MQ=255)
                                                                                            aaCAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTc                                           <  2:288882/149‑1 (MQ=255)
                                                                                            aaCAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTc                                           <  5:198985/149‑1 (MQ=255)
                                                                                             aaaGCACCTCAACACGGCGTGCGAAGAGGGCCAGACCCTTAGCGACATCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGACTGGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTc                                           <  1:78254/147‑1 (MQ=255)
                                                                                             aCAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAATGCGCGACCGTGAC‑GGCCGTGTACAACCTGGTTCTCTCCGAAGTCGAGGCGCCCctcctc                                           >  8:226991/1‑148 (MQ=255)
                                                                                                gCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGaaa                                       <  3:141872/149‑1 (MQ=255)
                                                                                                gCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGaaa                                       <  5:346757/149‑1 (MQ=255)
                                                                                                gCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGaaa                                       <  1:107507/149‑1 (MQ=255)
                                                                                                     tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGgcgc                                                   <  6:263026/132‑1 (MQ=255)
                                                                                                     tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGgcgc                                                   >  5:263026/1‑132 (MQ=255)
                                                                                                     tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAg                                      >  5:231091/1‑145 (MQ=255)
                                                                                                     tCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAg                                      <  6:231091/145‑1 (MQ=255)
                                                                                                            gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gc                                                                                                                               <  7:322257/50‑1 (MQ=255)
                                                                                                            gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑Gc                                                                                                                               >  8:322257/1‑50 (MQ=255)
                                                                                                            gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                                 >  2:241451/1‑143 (MQ=255)
                                                                                                            gCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                                 <  1:241451/143‑1 (MQ=255)
                                                                                                             ccGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTa                          >  6:147736/1‑149 (MQ=255)
                                                                                                                  cGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTg                      >  4:210716/1‑148 (MQ=255)
                                                                                                                      gagGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAggg                 >  3:4724/1‑149 (MQ=255)
                                                                                                                       agGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                                 >  2:199337/1‑132 (MQ=255)
                                                                                                                       agGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGtga                                 <  1:199337/132‑1 (MQ=255)
                                                                                                                           ccAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGaa                    <  6:2425/141‑1 (MQ=255)
                                                                                                                           ccAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAgagatcgg            >  5:2425/1‑142 (MQ=255)
                                                                                                                                gccTTCTCGACGCTGTCGCGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGAc        >  4:273510/2‑148 (MQ=255)
                                                                                                                                       cgACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGACCAAGGc  >  5:224135/1‑147 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
AGACAGAGGACGGACAAGGGGTGGCCGCATGACGATGATGACCGAGAATTTTGTGAGTGGAACAACGCCCGTGAGCGACAACGCCAACCTGAAACAGCACCTCAACACGCCGAGCGAAGAGGGCCAGACCCTTCGCGACAGCGTCGAGAAGGCG‑CT‑‑GCACAACTACTTCGCCCACCTGGAAGGCGCGACCGTGAC‑GGACGTGTACAACCTGGTGCTCTCCGAAGTCGAGGCGCCCCTGCTCGAAAGCGTGATGAACTACGTGAAGGGCAACCAGACCAAGGC  >  NC_002947/5483057‑5483338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑