Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,532,754 A→G 9.9% S144P (TCC→CCC)  braD ← high‑affinity branched‑chain amino acid ABC transporter permease BraD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,532,7540AG 9.9% 157.5 / 6.3 60S144P (TCC→CCC) braDhigh‑affinity branched‑chain amino acid ABC transporter permease BraD
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base G (4/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGGATCGAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGC  >  NC_002947/5532606‑5532899
                                                                                                                                                    |                                                                                                                                                   
aGGATCGAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGa                                                                                                                                                     <  7:215017/149‑1 (MQ=255)
 ggATCGAGGCCATCTTGCTGTCTTGCTGGGTAGCACGGAACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGACGGAc                                                                                                                                                    >  6:339529/1‑149 (MQ=255)
 ggATCGAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGAc                                                                                                                                                    >  6:333674/1‑149 (MQ=255)
      gAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGAc                                                                                                                                               <  8:28036/149‑1 (MQ=255)
         gCCATCTTGCGGTCTTGCTGGGTAGCACCGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTc                                                                                                                                            >  7:258156/1‑149 (MQ=255)
          ccATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCg                                                                                                                                           <  3:306223/149‑1 (MQ=255)
                 gCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGc                                                                                                                                     <  8:114326/148‑1 (MQ=255)
                       ttGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGTCGGGTCCGGCTTCgt                                                                                                                                          >  2:220525/1‑136 (MQ=255)
                       ttGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGTCGGGTCCGGCTTCgt                                                                                                                                          <  1:220525/137‑2 (MQ=255)
                       ttGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGAACGAAGCCGGACCCGACTTCGACACGCA‑T‑Ggcgc                                                                                                                            >  1:271559/1‑149 (MQ=255)
                         gCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAATTCAGTTGCACGAACCCGGACCCGACTTCGACACGCA‑T‑GGCGCCt                                                                                                                          >  6:169085/1‑149 (MQ=255)
                                  cACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACTCA‑T‑GGCGCCTTCCAGCAgg                                                                                                                 <  5:339529/149‑1 (MQ=255)
                                  cACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAgg                                                                                                                 <  8:258156/149‑1 (MQ=255)
                                    cGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGt                                                                                                               >  8:121214/1‑149 (MQ=255)
                                           tGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGa                                                                                                                                                >  2:291293/1‑111 (MQ=255)
                                           tGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGa                                                                                                                                                <  1:291293/111‑1 (MQ=255)
                                              ggCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCt                                                                                                     >  1:129120/1‑149 (MQ=255)
                                              ggCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCt                                                                                                     >  8:218893/1‑149 (MQ=255)
                                                  gAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCt                                                                                                 <  2:129120/149‑1 (MQ=255)
                                                        ggTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggcgg                                                                                            <  7:218893/148‑1 (MQ=255)
                                                        ggTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggcgg                                                                                            <  2:271559/148‑1 (MQ=255)
                                                              cTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTAGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCt                                                                                     <  2:73822/149‑1 (MQ=255)
                                                                 gATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGc                                                                                  >  7:287142/1‑149 (MQ=255)
                                                                          ggTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATT‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                          <  1:92028/149‑1 (MQ=255)
                                                                          ggTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑TCTGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                          <  1:96371/149‑1 (MQ=255)
                                                                              agcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTa                                                                     <  8:112864/149‑1 (MQ=255)
                                                                                cagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggc                                                                                              >  4:33669/1‑122 (MQ=255)
                                                                                cagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggc                                                                                              <  3:33669/122‑1 (MQ=255)
                                                                                      cATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTg                                                               >  6:309303/1‑147 (MQ=255)
                                                                                             aCAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGGACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGTGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAg                                                      <  5:169085/149‑1 (MQ=255)
                                                                                              cAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                     >  4:52409/1‑149 (MQ=255)
                                                                                               aaaGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                     >  8:291176/1‑148 (MQ=255)
                                                                                                     cgccACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGc                                              >  5:21300/1‑149 (MQ=255)
                                                                                                     cgccACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGc                                              <  3:131809/149‑1 (MQ=255)
                                                                                                        caccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGATACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                     >  7:39901/1‑139 (MQ=255)
                                                                                                        caccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGATACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                     <  8:39901/139‑1 (MQ=255)
                                                                                                           caGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATgg                                        >  7:279286/1‑149 (MQ=255)
                                                                                                                tGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGAt                                                         >  4:61539/1‑127 (MQ=255)
                                                                                                                tGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGAt                                                         <  3:61539/127‑1 (MQ=255)
                                                                                                                tGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACt                                    >  7:120210/1‑148 (MQ=255)
                                                                                                                   aGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATc                                <  3:52409/149‑1 (MQ=255)
                                                                                                                       cTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCg                            >  7:88647/1‑149 (MQ=255)
                                                                                                                       cTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCg                            >  8:335019/1‑149 (MQ=255)
                                                                                                                       cTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCg                            >  5:141228/1‑149 (MQ=255)
                                                                                                                        ttGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCgg                           >  2:56793/1‑149 (MQ=255)
                                                                                                                            tataAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTCGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGc                        >  2:37952/1‑148 (MQ=255)
                                                                                                                                 gTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACCGCTGATCAGTGCCatcgg                  <  6:21300/149‑5 (MQ=255)
                                                                                                                                  tCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCgg                 <  7:335019/149‑1 (MQ=255)
                                                                                                                                    aGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGt               >  2:142535/1‑149 (MQ=255)
                                                                                                                                     gTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTg              <  8:88647/149‑1 (MQ=255)
                                                                                                                                      ttGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTgg             <  1:142535/149‑1 (MQ=255)
                                                                                                                                       tGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTgg             <  7:121214/148‑1 (MQ=255)
                                                                                                                                        gCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGa            <  7:291176/148‑1 (MQ=255)
                                                                                                                                        gCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGa            <  6:141228/148‑1 (MQ=255)
                                                                                                                                        gCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGa            <  5:309303/148‑1 (MQ=255)
                                                                                                                                         gtcGAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGAACTGATCAGCGGTGCCAGGCGGGTGGAGt          >  7:36908/3‑149 (MQ=255)
                                                                                                                                          tcGAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGt          >  8:183546/2‑148 (MQ=255)
                                                                                                                                          tcGAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGt          >  3:10495/2‑148 (MQ=255)
                                                                                                                                            gAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTa        <  4:10495/148‑1 (MQ=255)
                                                                                                                                            gAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTAc       >  3:24997/1‑149 (MQ=255)
                                                                                                                                                 cGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGc  >  2:88873/1‑149 (MQ=255)
                                                                                                                                                    aCCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTg              <  4:179663/134‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGATCGAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGC  >  NC_002947/5532606‑5532899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑