Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,532,759 A→G 10.2% V142A (GTC→GCC)  braD ← high‑affinity branched‑chain amino acid ABC transporter permease BraD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,532,7590AG10.2% 152.0 / 7.7 59V142A (GTC→GCC) braDhigh‑affinity branched‑chain amino acid ABC transporter permease BraD
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base G (4/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

GAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTGTA  >  NC_002947/5532612‑5532907
                                                                                                                                                   |                                                                                                                                                      
gAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGAc                                                                                                                                                       <  8:28036/149‑1 (MQ=255)
   gCCATCTTGCGGTCTTGCTGGGTAGCACCGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTc                                                                                                                                                    >  7:258156/1‑149 (MQ=255)
    ccATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCg                                                                                                                                                   <  3:306223/149‑1 (MQ=255)
           gCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGc                                                                                                                                             <  8:114326/148‑1 (MQ=255)
                 ttGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGTCGGGTCCGGCTTCgt                                                                                                                                                  <  1:220525/137‑2 (MQ=255)
                 ttGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGTCGGGTCCGGCTTCgt                                                                                                                                                  >  2:220525/1‑136 (MQ=255)
                 ttGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGAACGAAGCCGGACCCGACTTCGACACGCA‑T‑Ggcgc                                                                                                                                    >  1:271559/1‑149 (MQ=255)
                   gCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAATTCAGTTGCACGAACCCGGACCCGACTTCGACACGCA‑T‑GGCGCCt                                                                                                                                  >  6:169085/1‑149 (MQ=255)
                            cACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACTCA‑T‑GGCGCCTTCCAGCAgg                                                                                                                         <  5:339529/149‑1 (MQ=255)
                            cACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAgg                                                                                                                         <  8:258156/149‑1 (MQ=255)
                              cGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGt                                                                                                                       >  8:121214/1‑149 (MQ=255)
                                     tGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGa                                                                                                                                                        <  1:291293/111‑1 (MQ=255)
                                     tGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGa                                                                                                                                                        >  2:291293/1‑111 (MQ=255)
                                        ggCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCt                                                                                                             >  1:129120/1‑149 (MQ=255)
                                        ggCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCt                                                                                                             >  8:218893/1‑149 (MQ=255)
                                            gAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCt                                                                                                         <  2:129120/149‑1 (MQ=255)
                                                  ggTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggcgg                                                                                                    <  2:271559/148‑1 (MQ=255)
                                                  ggTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggcgg                                                                                                    <  7:218893/148‑1 (MQ=255)
                                                        cTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTAGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCt                                                                                             <  2:73822/149‑1 (MQ=255)
                                                           gATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGc                                                                                          >  7:287142/1‑149 (MQ=255)
                                                                    ggTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCATT‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                                  <  1:92028/149‑1 (MQ=255)
                                                                    ggTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑TCTGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCtgt                                                                                  <  1:96371/149‑1 (MQ=255)
                                                                        agcagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTa                                                                             <  8:112864/149‑1 (MQ=255)
                                                                          cagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggc                                                                                                      <  3:33669/122‑1 (MQ=255)
                                                                          cagGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTggc                                                                                                      >  4:33669/1‑122 (MQ=255)
                                                                                cATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTg                                                                       >  6:309303/1‑147 (MQ=255)
                                                                                       aCAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGGACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGTGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAg                                                              <  5:169085/149‑1 (MQ=255)
                                                                                        cAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                             >  4:52409/1‑149 (MQ=255)
                                                                                         aaaGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                             >  8:291176/1‑148 (MQ=255)
                                                                                               cgccACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGc                                                      <  3:131809/149‑1 (MQ=255)
                                                                                               cgccACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGc                                                      >  5:21300/1‑149 (MQ=255)
                                                                                                  caccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGATACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                             >  7:39901/1‑139 (MQ=255)
                                                                                                  caccaGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGATACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGc                                                             <  8:39901/139‑1 (MQ=255)
                                                                                                     caGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATgg                                                >  7:279286/1‑149 (MQ=255)
                                                                                                          tGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGAt                                                                 <  3:61539/127‑1 (MQ=255)
                                                                                                          tGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGAt                                                                 >  4:61539/1‑127 (MQ=255)
                                                                                                          tGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACt                                            >  7:120210/1‑148 (MQ=255)
                                                                                                             aGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATc                                        <  3:52409/149‑1 (MQ=255)
                                                                                                                 cTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCg                                    >  7:88647/1‑149 (MQ=255)
                                                                                                                 cTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCg                                    >  5:141228/1‑149 (MQ=255)
                                                                                                                 cTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCg                                    >  8:335019/1‑149 (MQ=255)
                                                                                                                  ttGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCgg                                   >  2:56793/1‑149 (MQ=255)
                                                                                                                      tataAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTCGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGc                                >  2:37952/1‑148 (MQ=255)
                                                                                                                           gTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACCGCTGATCAGTGCCatcgg                          <  6:21300/149‑5 (MQ=255)
                                                                                                                            tCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCgg                         <  7:335019/149‑1 (MQ=255)
                                                                                                                              aGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGt                       >  2:142535/1‑149 (MQ=255)
                                                                                                                               gTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTg                      <  8:88647/149‑1 (MQ=255)
                                                                                                                                ttGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTgg                     <  1:142535/149‑1 (MQ=255)
                                                                                                                                 tGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTgg                     <  7:121214/148‑1 (MQ=255)
                                                                                                                                  gCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGa                    <  7:291176/148‑1 (MQ=255)
                                                                                                                                  gCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGa                    <  6:141228/148‑1 (MQ=255)
                                                                                                                                  gCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGa                    <  5:309303/148‑1 (MQ=255)
                                                                                                                                   gtcGAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGAACTGATCAGCGGTGCCAGGCGGGTGGAGt                  >  7:36908/3‑149 (MQ=255)
                                                                                                                                    tcGAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGt                  >  8:183546/2‑148 (MQ=255)
                                                                                                                                    tcGAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGt                  >  3:10495/2‑148 (MQ=255)
                                                                                                                                      gAAGTCGGGTCCGGCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTa                <  4:10495/148‑1 (MQ=255)
                                                                                                                                      gAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTAc               >  3:24997/1‑149 (MQ=255)
                                                                                                                                           cGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGc          >  2:88873/1‑149 (MQ=255)
                                                                                                                                              aCCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTg                      <  4:179663/134‑1 (MQ=255)
                                                                                                                                                ccGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGttt     <  4:172922/149‑1 (MQ=255)
                                                                                                                                                ccGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGttt     >  2:186075/1‑149 (MQ=255)
                                                                                                                                                   aCTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTCGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTGTa  <  1:37952/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GAGGCCATCTTGCGGTCTTGCTGGGTAGCACGGCACATGCGGCCGAGCTTGGTGTACTTGATCACGTAGGTCAGCAGGCCCATGCCGACAAAGGCCGCCACCAGGATGAAGATCTTGGTATAAGTCAGTTGCACGAAGCCGGACCCGACTTCGACACGCA‑T‑GGCGCCTTCCAGCAGGGTTGGCACGCCTTGCTGGCGGGCGCCCTGGCTGATCTGTGCGTAGTTCTGCAGGATCAGCGAAATGCCGATGGCACTGATCAGCGGTGCCAGGCGGGTGGAGTTACGCAGCGGTTTGTA  >  NC_002947/5532612‑5532907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑