Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 516,693 T→G 7.0% E100A (GAA→GCA) ‡ PP_0429 ← short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947516,6930TG 7.0% 176.3 / 4.1 57E100A (GAA→GCA) ‡PP_0429short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (27/26);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

ATCCATGGGCCAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACG  >  NC_002947/516546‑516839
                                                                                                                                                   |                                                                                                                                                   
atccatGGGCCAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTc                                                                                                                                                    <  5:128794/149‑1 (MQ=255)
    atGGGCCAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATGAATgg                                                                                                                                                >  3:51470/1‑149 (MQ=255)
        gCCAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCa                                                                                                                                            <  6:55974/149‑1 (MQ=255)
        gCCAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATGAATGGGccg                                                                                                                                            <  2:116019/149‑2 (MQ=255)
         ccAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATGAATGGGccga                                                                                                                                           <  4:51470/149‑3 (MQ=255)
               gcCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCGTGTTCGTCGGCCCATTCATGGGCCAGGAACgg                                                                                                                                     <  4:230015/149‑1 (MQ=255)
               gcCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACgg                                                                                                                                     >  3:119036/1‑149 (MQ=255)
               gcCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACgg                                                                                                                                     >  8:189703/1‑149 (MQ=255)
                 cACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGcc                                                                                                                                   <  4:119036/149‑1 (MQ=255)
                      cGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAg                                                                                                                                           >  3:32809/1‑136 (MQ=255)
                      cGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAg                                                                                                                                           <  4:32809/136‑1 (MQ=255)
                        gCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGt                                                                                                                            <  1:300891/149‑1 (MQ=255)
                        gCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGt                                                                                                                            <  1:296033/149‑1 (MQ=255)
                                  cGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGccc                                                                                                                  <  2:317907/149‑1 (MQ=255)
                                          agaATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCAt                                                                                                                                >  6:34293/1‑127 (MQ=255)
                                          agaATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCAt                                                                                                                                <  5:34293/127‑1 (MQ=255)
                                          agaATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGtt                                                                                                          <  7:189703/149‑1 (MQ=255)
                                                     gCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑t                                                                                              >  4:87783/1‑149 (MQ=255)
                                                          ccTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATa                                                                                         >  4:102357/1‑149 (MQ=255)
                                                                    cATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGc                                                                               >  6:307691/1‑149 (MQ=255)
                                                                         ggTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCcttct                                                                          >  6:202110/1‑149 (MQ=255)
                                                                          gTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCcttctt                                                                         >  8:82938/1‑149 (MQ=255)
                                                                              ccGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCa                                                                      <  3:102357/148‑1 (MQ=255)
                                                                               cGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGa                                                                                                        >  2:184216/1‑114 (MQ=255)
                                                                               cGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGa                                                                                                        <  1:184216/114‑1 (MQ=255)
                                                                                          cacaGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACg                                                           >  4:150391/1‑147 (MQ=255)
                                                                                           acaGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCAGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAg                                                        <  5:307691/149‑1 (MQ=255)
                                                                                            caGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGc                                                       <  3:87783/149‑1 (MQ=255)
                                                                                                  gcTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGaaa                                                 <  7:82938/149‑1 (MQ=255)
                                                                                                    tCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAgg                                               >  2:126281/1‑149 (MQ=255)
                                                                                                        ggCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCcgc                                                                                                                <  6:196134/81‑1 (MQ=255)
                                                                                                        ggCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCcgc                                                                                                                >  5:196134/1‑81 (MQ=255)
                                                                                                        ggCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGgcg                                             >  2:154012/1‑147 (MQ=255)
                                                                                                           catcACGTTGACTGCCAGCAGATCCTGTGCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGcc                                                      <  2:17865/132‑1 (MQ=255)
                                                                                                           cAGCACGTTGACTACCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTACTTCCAGCTCTAGCACGAAGcc                                                      >  1:17865/1‑135 (MQ=255)
                                                                                                                 gTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGa                                                          <  7:83772/125‑1 (MQ=255)
                                                                                                                 gTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGa                                                          >  8:83772/1‑125 (MQ=255)
                                                                                                                 gTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCggctggc                                  >  2:248470/1‑149 (MQ=255)
                                                                                                                  ttGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCggctggct                                 <  3:150391/149‑1 (MQ=255)
                                                                                                                     aCTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGa                               <  6:196386/148‑1 (MQ=255)
                                                                                                                      cTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAgg                             >  5:225993/1‑149 (MQ=255)
                                                                                                                       ttCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAg                                                        <  3:107648/121‑1 (MQ=255)
                                                                                                                       ttCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAg                                                        >  4:107648/1‑121 (MQ=255)
                                                                                                                          cagcagATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCg                          >  6:301647/1‑148 (MQ=255)
                                                                                                                           agcagATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCg                          >  7:115254/1‑147 (MQ=255)
                                                                                                                            gcagATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAg                       <  5:301647/149‑1 (MQ=255)
                                                                                                                             cagATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAgg                      <  1:154012/149‑1 (MQ=255)
                                                                                                                                   cTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCgg                 >  2:248639/1‑148 (MQ=255)
                                                                                                                                        cgtcgGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCgg                                       <  7:143835/121‑1 (MQ=255)
                                                                                                                                        cgtcgGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCgg                                       >  8:143835/1‑121 (MQ=255)
                                                                                                                                           tggCCCATGAATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCAcc        >  7:89134/2‑149 (MQ=255)
                                                                                                                                            ggCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCt       >  1:166903/1‑149 (MQ=255)
                                                                                                                                            ggCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTCAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCt       <  1:248639/149‑1 (MQ=255)
                                                                                                                                               ccATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTc                           >  5:162898/1‑126 (MQ=255)
                                                                                                                                               ccATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTc                           <  6:162898/126‑1 (MQ=255)
                                                                                                                                                 aTTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAATTCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGAc   >  1:270545/1‑149 (MQ=255)
                                                                                                                                                 aTTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACg  >  2:327861/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCCATGGGCCAGGCGCCACCCCGGCCAGGCCGGCGACGTTGAGAATCTGCCCGCCACCCTGTACAGCCATCAGGTTACCGATGGCATGGCACAGGCGGCTCAGGGCCAGCACGTTGACTTCCAGCAGATCCTGTTCGTCGGCCCATTCATGGGCCAGGAACGGCCCATAGGTGCGCAAGCCCGCGCAGTTGACCAGCAAA‑TCGATACGCCGCTCGCCTTCTTCCAGCTCTAGCACGAAGCCTGAAAGGCGCAGCGGCTGGCTGAGGTCGCAGGCGCGGAACAGCACCTCGACG  >  NC_002947/516546‑516839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑