| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,549,605 | T→A | 13.4% | intergenic (+18/‑154) | PP_t47 → / → PP_4881 | tRNA‑Leu/iron ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,549,605 | 0 | T | A | 13.4% | 64.6 / 3.9 | 30 | intergenic (+18/‑154) | PP_t47/PP_4881 | tRNA‑Leu/iron ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (10/16); new base A (2/2); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01 | |||||||||||
AACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGG > NC_002947/5549465‑5549748 | aaCGATAGTCGCCTTCTGCAGATGATGTTAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGTg > 1:193770/1‑149 (MQ=255)aaCGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTg < 3:141572/149‑1 (MQ=255) aCGATAGTCGCCTTCTGCAGATGATGTTAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGTgg < 2:193770/149‑1 (MQ=255) aTAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGtt > 3:79015/1‑149 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTgg > 1:84935/1‑123 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTgg < 2:84935/123‑1 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGt > 6:230804/1‑121 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGt < 5:230804/121‑1 (MQ=255) aatgtgtGCCCAGGTGGCGGAAGTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCAAGTGGGTTTTTTCTTGCCTGCGATTTATGTGc < 6:40847/142‑1 (MQ=255) gTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGATAGTGGGTTTTTTCTTGCCTGCGATTTAt > 8:218492/1‑107 (MQ=255) catgTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTtctc < 1:334867/145‑1 (MQ=255) cAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTc < 8:188782/113‑1 (MQ=255) gTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTTGTCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATTGTCTGACCATGAATTTCTCGTCACACGGc < 1:102271/149‑1 (MQ=255) gTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGGCCCCGCCGCCCCTGGCACCATGGTCGGCCCATGa > 4:332954/1‑132 (MQ=38) gTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGa < 3:332954/132‑1 (MQ=255) gTGACAGCAAGGTCGTGGAAGTTCGAGTCATCTCCTGGGCACCATACAAAAACCAACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGGCACGGGCCCCCCGTGCACCATGTGCTGAAAATCAAGATCTCTTCacacag > 2:102271/1‑146 (MQ=25) gTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGaaa < 7:2278/149‑1 (MQ=255) tGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCGCCCTCCCCCCCTTGCACCATGGGCTGACCATGAAGTTAGAGTCGCACGGCCAGTTGTGAAACAAt > 8:260761/1‑149 (MQ=255) gTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGt > 6:45289/1‑149 (MQ=255) gAGTCTTCTCCTTGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTAc < 3:236404/149‑1 (MQ=255) gAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGAACATGAATTTCACGTCAAACTGCCACTTGAGAAACAAATTcatata > 4:236404/1‑143 (MQ=255) aGTCATCTCCAGGGCACCATTCCAAAACCCCCTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTAcc < 8:82689/149‑1 (MQ=255) ttgtcCTTGTCACCATACAAATACCCACTATCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCGCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTc < 7:73603/135‑1 (MQ=255) ggCACCATACAAAAACCCACTATCTTGCTAGTGGTTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTAcc < 7:148404/137‑1 (MQ=255) cAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGcc < 2:160704/149‑1 (MQ=255) cACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGCTTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGa < 4:79015/149‑1 (MQ=255) cACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGtt > 2:283283/1‑116 (MQ=255) cACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGa < 5:45289/149‑1 (MQ=255) cACTAGCTTGCTAGTGGGTTATTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGtt < 1:283283/116‑1 (MQ=255) tAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCGCCCCCCCGTGCCCCATGGTCTGCCCATGAATTTCTCGTCACACGGGCACTTGAGAAACAATTTGGTTTAGCATTGTTTGCATATTTGTAGCCGTAATGGAgg > 8:153959/1‑148 (MQ=255) | AACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGG > NC_002947/5549465‑5549748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |