Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,584,672 A→T 9.3% L354M (TTG→ATG)  parC ← DNA topoisomerase IV subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,584,6720AT 9.3% 118.5 / 3.3 43L354M (TTG→ATG) parCDNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base A (22/17);  new base T (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGTTGGATCGCGGGATG  >  NC_002947/5584530‑5584814
                                                                                                                                              |                                                                                                                                              
tggATGAAGTGATCCACATCATCCAGGGTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGCACGGTATTGGTGCGGAACTCAAGCCACTCCACCAATAGGGt                                                                                                                                          >  3:197/2‑149 (MQ=255)
        gtgGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCg                                                                                                                                       >  1:210069/1‑144 (MQ=255)
        gtgGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCg                                                                                                                                       <  2:210069/144‑1 (MQ=255)
                    aTCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGc                                                                                                                       <  4:345855/148‑1 (MQ=255)
                                gAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTc                                                                                                            >  5:168539/1‑147 (MQ=255)
                                     cAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGcc                                                                                                      <  6:168539/148‑1 (MQ=255)
                                                 cGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACg                                                                                         >  8:167120/1‑149 (MQ=255)
                                                 cGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACg                                                                                         >  1:339829/1‑149 (MQ=255)
                                                   tCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAg                                                                                                         <  4:69918/131‑1 (MQ=255)
                                                   tCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAg                                                                                                         >  3:69918/1‑131 (MQ=255)
                                                            tGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATa                                                                              <  2:339829/149‑1 (MQ=255)
                                                             gCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCATTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAg                                                                             >  6:259734/1‑149 (MQ=255)
                                                             gCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGgccggccg                                                                                                              >  6:67160/1‑116 (MQ=255)
                                                             gCAGACGCTTCTCGACCTTAACCCGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGgccggccg                                                                                                              <  5:67160/116‑1 (MQ=255)
                                                               aGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACTGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGc                                                                                                       <  8:137497/121‑1 (MQ=255)
                                                               aGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGc                                                                                                       >  7:137497/1‑121 (MQ=255)
                                                               aGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGt                                                                           <  7:167120/149‑1 (MQ=255)
                                                               aGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGt                                                                           <  4:104562/149‑1 (MQ=255)
                                                               aGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGt                                                                           >  3:104562/1‑149 (MQ=255)
                                                                 aCGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCAGATAGGTGc                                                                         >  6:269834/1‑149 (MQ=255)
                                                                   gCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCtt                                                                       >  2:271842/1‑149 (MQ=255)
                                                                        tcGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAg                                                                  >  4:174972/1‑149 (MQ=255)
                                                                             cTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCt                                                                        <  4:93820/138‑1 (MQ=255)
                                                                             cTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCt                                                                        >  3:93820/1‑138 (MQ=255)
                                                                                   cAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGcggcc                                                                                                                   >  6:62114/1‑89 (MQ=255)
                                                                                   cAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGcggcc                                                                                                                   <  5:62114/89‑1 (MQ=255)
                                                                                   cAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAgg                                                                            >  5:296973/1‑128 (MQ=255)
                                                                                   cAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAgg                                                                            <  6:296973/128‑1 (MQ=255)
                                                                                    aGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGcggccgg                                                                                                                 <  1:173997/90‑1 (MQ=255)
                                                                                    aGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGcggccgg                                                                                                                 >  2:173997/1‑90 (MQ=255)
                                                                                    aGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGc                                                      >  6:361337/1‑149 (MQ=255)
                                                                                        gATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAAc                                                  <  1:271842/149‑1 (MQ=255)
                                                                                         aTGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGaa                                                   >  8:105722/1‑147 (MQ=255)
                                                                                           gctgcAGTCGGCGACGAACGGTATTTGTGCGGAACTCCAGCCACTCCACCCATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAg                                                <  4:197/148‑1 (MQ=255)
                                                                                               cAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCATTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCGAGGTCGGTGGTAGCGAACAGGtgc                                            <  5:259734/148‑1 (MQ=255)
                                                                                                           aaCGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCtcgt                               <  3:174972/149‑1 (MQ=255)
                                                                                                           aaCGGTATTGGTGCGGAACTCCAGCCACTCCACAAATAGGGTTCGCAGGTTTTTCAACTGCGGACGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCtcgt                               >  1:98747/1‑149 (MQ=255)
                                                                                                                                cAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGTTgagat          >  5:355574/1‑145 (MQ=255)
                                                                                                                                cAGCCACTCAACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGtt               <  6:355574/144‑1 (MQ=255)
                                                                                                                                       tCCACCATTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCggtg                                                           <  6:187159/93‑1 (MQ=255)
                                                                                                                                       tCCACCATTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCggtg                                                           >  5:187159/1‑93 (MQ=255)
                                                                                                                                       tCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGTTGGATCGCGGgat   >  6:127892/1‑149 (MQ=255)
                                                                                                                                         ctcccaTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCCCGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGTTGGATCGCGGgatg  <  5:350728/143‑1 (MQ=255)
                                                                                                                                            caATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGTTGGATCGCGGgatg  >  6:350728/1‑145 (MQ=255)
                                                                                                                                              aTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCTATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCTAACAGGTGCTGCATc                                      <  4:60184/107‑1 (MQ=255)
                                                                                                                                              aTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCggtg                                                           >  4:327442/1‑86 (MQ=255)
                                                                                                                                              aTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCggtg                                                           <  3:327442/86‑1 (MQ=255)
                                                                                                                                              aTAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATc                                      >  3:60184/1‑107 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CGGATGATGTGGATCACTTCATCCAGGTTGAGGAATGCAGTGAGCAAACCGTCCAACAGGTGCAGACGCTTCTCGACCTTATCCAGACGATGCTGCAGGCGGCGACGAACGGTATTGGTGCGGAACTCCAGCCACTCCACCAATAGGGTTCGCAGGTTTTTCAACTGCGGCCGGCCGTCCAGGCCGATGATATTCACGTTGACCCGATAGGTGCTTTCCAGGTCGGTGGTAGCGAACAGGTGCTGCATCAGCTCGTCGACATCGACGCGGTTGGATCGCGGGATG  >  NC_002947/5584530‑5584814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑