Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,614,703	A→C	6.4%	I273M (ATT→ATG)	hldE ←	bifunctional heptose 7‑phosphate kinase/heptose 1‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,614,703	0	A	C	6.4%	194.1 / 4.7	62	I273M (ATT→ATG)	hldE	bifunctional heptose 7‑phosphate kinase/heptose 1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (31/27);  new base C (2/2);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

AGCACGCCGCGTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGACCAGCAAG  >  NC_002947/5614557‑5614844                                                                                                                                                    |                                                                                                                                              aGCACGCCGCGTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAAt                                                                                                                                             >  3:251734/1‑149 (MQ=255)        cgcgTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCCATTGCcgcg                                                                                                                                      >  3:62934/1‑149 (MQ=255)         gcgTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCCATTGCCGCgg                                                                                                                                     <  4:62934/149‑1 (MQ=255)         gcgTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCCATTGCCGCgg                                                                                                                                     <  5:173525/149‑1 (MQ=255)         gcgTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCgg                                                                                                                                     >  7:37445/1‑149 (MQ=255)               ggAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAgg                                                                                                                                <  3:135494/148‑1 (MQ=255)                 aGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTg                                                                                                                             <  4:251734/149‑1 (MQ=255)                 aGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTg                                                                                                                             <  3:321218/149‑1 (MQ=255)                 aGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTg                                                                                                                             <  2:280810/149‑1 (MQ=255)                       ccTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCTGCCGTACCCAGCTGCCCGACCACAATGCCCGCTGCCAGGTTCTGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGaaa                                                                                                                         <  1:100425/148‑1 (MQ=255)                           aCGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACa                                                                                                                    <  4:150206/148‑1 (MQ=255)                           aCGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAg                                                                                                                   >  8:109153/1‑149 (MQ=255)                             gCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTa                                                                                                                 >  5:124783/1‑149 (MQ=255)                               tGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGt                                                                                                                  >  5:259832/1‑146 (MQ=255)                               tGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGt                                                                                                                  <  6:259832/146‑1 (MQ=255)                               tGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATc                                                                                                               >  1:144366/1‑149 (MQ=255)                                 gATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGaaa                                                                                                                         >  6:310713/1‑137 (MQ=255)                                 gATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGaaa                                                                                                                         <  5:310713/137‑1 (MQ=255)                                 gATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGc                                                                                                             >  8:236857/1‑149 (MQ=255)                                      cccGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGaaa                                                                                                                         >  4:142745/1‑132 (MQ=255)                                      cccGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGaaa                                                                                                                         <  3:142745/132‑1 (MQ=255)                                       ccGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCcgc                                                                                                                                       <  4:308856/117‑1 (MQ=255)                                       ccGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCcgc                                                                                                                                       >  3:308856/1‑117 (MQ=255)                                       ccGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATc                                                                                                               >  6:21011/1‑141 (MQ=255)                                       ccGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATc                                                                                                               <  5:21011/141‑1 (MQ=255)                                                 tCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATc                                                                                             >  8:187696/1‑149 (MQ=255)                                                     gggCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACa                                                                                                                    >  6:30101/1‑122 (MQ=255)                                                       gcgcTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAacac                                                                                       >  6:53837/1‑149 (MQ=255)                                                        cgcTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACt                                                                                      >  5:127692/1‑149 (MQ=255)                                                           tGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGCACACCGAATGGGGCAGGACCTCACCCGAGGCAATGTCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTAACAACGACCACTTcg                                                                                   >  7:333807/1‑149 (MQ=255)                                                             aTGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTcgc                                                                                  <  8:37445/148‑1 (MQ=255)                                                             aTGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTcgc                                                                                  <  7:236857/148‑1 (MQ=255)                                                               ggCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTg                                                                               >  7:272512/1‑149 (MQ=255)                                                                            aGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCgcaggcag                                                                  <  2:144366/149‑1 (MQ=255)                                                                                 gCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGc                                                              <  6:127692/148‑1 (MQ=255)                                                                                   cGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAAc                                                           >  5:175057/1‑149 (MQ=255)                                                                                         caATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCg                                                                                                     >  2:175758/1‑101 (MQ=255)                                                                                         caATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCg                                                                                                     <  1:175758/101‑1 (MQ=255)                                                                                         caATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTACTCACACGCGGCAATTGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCAACTGTCACAACGAACACTTCGCGTGCCATCGCAGGCAGGTGCAACGCCTGc                                                     >  6:194013/1‑149 (MQ=255)                                                                                          aaTGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCt                                                    >  6:73827/1‑149 (MQ=255)                                                                                              cccGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAAAGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTGACATCGAACACTTCGCGTGCCCGCGCAGTCACGt                                                                >  8:116039/1‑133 (MQ=255)                                                                                                         gTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACa                                     <  5:73827/149‑1 (MQ=255)                                                                                                          tt‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAg                                    <  7:109153/149‑1 (MQ=255)                                                                                                               ccAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGt                                 >  7:143170/1‑148 (MQ=255)                                                                                                                 aGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcat                              >  8:101929/1‑149 (MQ=255)                                                                                                                    gCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGc                                                     <  2:117362/123‑1 (MQ=255)                                                                                                                    gCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGc                                                     <  4:195754/123‑1 (MQ=255)                                                                                                                    gCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcat                              <  5:139148/146‑1 (MQ=255)                                                                                                                    gCCACCGCATGGGGAAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcat                              >  6:139148/1‑146 (MQ=255)                                                                                                                     ccACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcatgcca                          <  7:101929/149‑1 (MQ=255)                                                                                                                       aCCGCGTGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcatgccttg                        >  3:61292/1‑149 (MQ=255)                                                                                                                                ggCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAac                                             <  2:122268/119‑1 (MQ=255)                                                                                                                                 gCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcat                              >  2:2975/1‑133 (MQ=255)                                                                                                                                     gTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGa          >  8:90802/1‑149 (MQ=255)                                                                                                                                      tCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAgg                                   <  7:94426/123‑1 (MQ=255)                                                                                                                                      tCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAgg                                   >  8:94426/1‑123 (MQ=255)                                                                                                                                      tCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGAc         >  5:186294/1‑149 (MQ=255)                                                                                                                                          ttgccGCGGCCATTGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCACCGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGACCAg      >  3:102692/4‑148 (MQ=255)                                                                                                                                           aCCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcatgccat                         >  7:167999/1‑128 (MQ=255)                                                                                                                                           aCCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTcatgccat                         <  8:167999/128‑1 (MQ=255)                                                                                                                                            cccGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGACCAGCaa   <  7:187696/149‑1 (MQ=255)                                                                                                                                             ccGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGACCAGCAAg  <  6:175057/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              AGCACGCCGCGTTCGGAGCCTTCCTCACGCTGGATGGCCCGGCGCAGCTCAGGGGCGCTGATGGCAGCCGTACCCAGCTTGCCGACCACAATGCCCGCTGCCAGGTT‑AGCCAGGGCCACCGCATGGGGCAGGTCCTCACCCGCGGCAATGGCCGCGGCAAGGGTGGAAATGACAGTATCGCCGGCACCGGTCACATCGAACACTTCGCGTGCCCGCGCAGGCAGGTGCAACGCCGGCTGACCAACACGCAACAGGGTCATGCCATGCTCACCGCGGGTGACCAGCAAG  >  NC_002947/5614557‑5614844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑