Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,629,625 G→T 15.3% A214D (GCT→GAT)  putP ← sodium/L‑proline transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,629,6250GT15.3% 78.5 / 2.9 35A214D (GCT→GAT) putPsodium/L‑proline transporter
Reads supporting (aligned to +/- strand):  ref base G (14/15);  new base T (3/2);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTGGTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTATTGTCTTCG  >  NC_002947/5629478‑5629768
                                                                                                                                                   |                                                                                                                                                
gTCTGGTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGc                                                                                                                                                 >  5:323595/1‑149 (MQ=255)
             cACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGTCTATGGTCGACGCCGCACCAGCCCAAAAAGCGGtt                                                                                                                                    >  8:259834/1‑149 (MQ=255)
             cACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGAGAAGGTCGCCGCCGCACCAGAACAAAAATCGGtt                                                                                                                                    >  2:104579/1‑149 (MQ=255)
                   tGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTAGCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTAc                                                                                                                              <  8:85197/149‑1 (MQ=255)
                            gCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCg                                                                                                                     <  6:323595/149‑1 (MQ=255)
                               cGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCTGTTTCGTACGACATGCCGAA‑g                                                                                                                 <  5:44635/149‑1 (MQ=255)
                                           tCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGACGATGGTCGACGCCGAAACAGAAAAAAAATATGTTTCGTACGACATGCCGAA‑GGTGCTCTCGaa                                                                                                      >  6:187571/1‑148 (MQ=255)
                                               gATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGAc                                                                                                 >  5:321520/1‑149 (MQ=255)
                                                   aGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACggg                                                                                              <  2:42537/148‑1 (MQ=255)
                                                    gcgcGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAATCCACCAACGAAGGTGTAGAACAAGGTCGACGCCGAAACATAAAAAAAAAATTTTTCGTACGACATTCCGAA‑GGTGCTCTCGAACAGACGGGCa                                                                                            >  6:220229/1‑149 (MQ=255)
                                                                tcaGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGtt                                                                                                                                    <  2:116100/98‑1 (MQ=255)
                                                                         ccTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGaa                                                                                                      <  6:305683/118‑1 (MQ=255)
                                                                         ccTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGaa                                                                                                      >  5:305683/1‑118 (MQ=255)
                                                                         ccTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGca                                                                       <  5:187571/149‑1 (MQ=255)
                                                                             tACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GATGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAg                                                                    >  7:76705/1‑148 (MQ=255)
                                                                                          cAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGAACAAGGTCGACGCCGAATCATTAAAAAAAAAAATATCGTACGACATGCGCAA‑GGTGCTCTCGAACAGCCGGGCACCGGCCACGATGCCGGAGGCAGAGTAGATGGTgaagaa                                                      >  6:146390/1‑149 (MQ=255)
                                                                                            gCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGc                                                                               >  5:292241/1‑122 (MQ=255)
                                                                                            gCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGc                                                                               <  6:292241/122‑1 (MQ=255)
                                                                                              tCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAacac                                                   >  3:72156/1‑148 (MQ=255)
                                                                                                aCCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCa                                                 <  2:65051/148‑1 (MQ=255)
                                                                                                aCCGCCAGGAAGCCACCAACCAAGGTGTAGAACAAGGTCGACGCCGAAACAAAAAAAAAAAAAGTATCCAACTACATGCCGAA‑GGTGCTCTCGCTCCGACCGGCACCGGCCACGAAGGCGGCGGCCCAGTAGATGGTGAAGAAcagcag                                                >  4:238859/1‑149 (MQ=255)
                                                                                                  cGCCAGGAAGCCACCACAGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGa                                              <  7:259834/149‑1 (MQ=255)
                                                                                                  cGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCATCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAACAACACCAGGa                                              <  5:146390/149‑1 (MQ=255)
                                                                                                   gCCAGGAATCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCATCCCACAAAGCGGTTTCGTACGACATGCCGAATGTTTCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGa                                              <  1:104579/149‑1 (MQ=255)
                                                                                                                caACGAAGGTGTAGAACAAGGTCGACGCCGAAACATAAAAAAAAAAGCTTTCGTACGACATGCCGAA‑GGTGCTCTCGCACCGACGGGCACCGGCCACGATGCCGGAGGCTCAGTAGATGGTGAAGCACCCCAGGAGGACGCTGGCGGa                                 >  6:282780/1‑148 (MQ=255)
                                                                                                                 aaCGAATGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGaaa                               <  5:220229/149‑1 (MQ=255)
                                                                                                                  aCGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GATGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAAt                              <  8:76705/149‑1 (MQ=255)
                                                                                                                    gAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGa                            >  3:133896/1‑149 (MQ=255)
                                                                                                                                   ggTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGa                                           <  1:187564/119‑1 (MQ=255)
                                                                                                                                   ggTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGa                                           >  2:187564/1‑119 (MQ=255)
                                                                                                                                     tCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCa                                                 <  4:226713/111‑1 (MQ=255)
                                                                                                                                     tCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCa                                                 >  3:226713/1‑111 (MQ=255)
                                                                                                                                       gccgccgcACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTAtt         <  5:282780/149‑1 (MQ=255)
                                                                                                                                        ccgccgcACCAGCCCACAAAGCGGTTTCGTACGACCTGCCGCA‑GGTGCTCGCGAACAGACGGGCACCGGCCCCGATGCCGGAGGCACCGTAGATGGTGAAGAACACCCGGATGACGATGGCGGAAATGATACGCAGCCGCCCGCGATTg        >  5:245711/1‑149 (MQ=255)
                                                                                                                                               aCCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTATTGTCTTCg  >  5:225691/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTCTGGTCGAAGCCACCGGTGGAGATCAGCACGATTACCGGGGTCAGGATCAGCGCGAAGATCATCAGCGAAGCCTGTACGGTATCGGTCCAGCTCACCGCCAGGAAGCCACCAACGAAGGTGTAGGCGATGGTCGCCGCCGCACCAGCCCACAAAGCGGTTTCGTACGACATGCCGAA‑GGTGCTCTCGAACAGACGGGCACCGGCCACGATGCCGGAGGCACAGTAGATGGTGAAGAACACCAGGATGACGATGGCGGAAATGATACGCAGCAGCCCGCTATTGTCTTCG  >  NC_002947/5629478‑5629768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑