Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,643,275	A→G	7.9%	intergenic (+60/‑63)	dbpA → / → yhiN	23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,643,275	0	A	G	7.9%	178.6 / 4.8	63	intergenic (+60/‑63)	dbpA/yhiN	23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base G (3/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

CGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGG  >  NC_002947/5643139‑5643422                                                                                                                                         |                                                                                                                                                     cgcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          <  6:179750/149‑1 (MQ=255) cgcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          <  2:108383/149‑1 (MQ=255)   cTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGt                                                                                                                                        <  7:26477/149‑1 (MQ=255)        cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAcc                                                                                                                                   >  6:78107/1‑149 (MQ=255)        cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAcc                                                                                                                                   >  3:246180/1‑149 (MQ=255)           gCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                                                                <  8:231042/149‑1 (MQ=255)                   tGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAg                                                                                                                        >  6:319430/1‑149 (MQ=255)                      aGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGt                                                                                                                     >  7:259958/1‑149 (MQ=255)                            tGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACACGCGAATGCGGCAGTGAATTCACCATCGCATTCGCGGGTGAACACTCTCCCACAGGGTTctcca                                                                                                               >  8:297665/1‑145 (MQ=38)                             gAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAt                                                                                                              >  4:306615/1‑149 (MQ=255)                               aCAGCGGCAAGATCATGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                                                             >  2:115513/1‑148 (MQ=255)                               aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                  <  8:105732/143‑1 (MQ=255)                               aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                  >  7:105732/1‑143 (MQ=255)                               aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                            <  5:78107/149‑1 (MQ=255)                                cAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                           <  8:259958/149‑1 (MQ=255)                                    ggCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑t                                                                                                      <  2:69210/149‑1 (MQ=255)                                     gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑Tc                                                                                                     >  5:364270/1‑149 (MQ=255)                                         gATCAAGGGTCGCGCCCTGAAAGTACTCATTATCTATAGCCAGGCAAAGCCCCCTGTATTAGCGGGTTTACCCGCTAATGCTTCAGTGAATTCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGaa                                                                                                 <  7:297665/149‑1 (MQ=37)                                          aTCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                                      <  2:63724/128‑1 (MQ=255)                                          aTCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                                      >  1:63724/1‑128 (MQ=255)                                           tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                        <  8:100238/141‑1 (MQ=255)                                           tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                        >  7:100238/1‑141 (MQ=255)                                           tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAgg                                                                                               <  5:319430/149‑1 (MQ=255)                                             aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGtt                                                                                             <  4:246180/149‑1 (MQ=255)                                                    gcgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                        <  5:280698/132‑1 (MQ=255)                                                    gcgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                        >  6:280698/1‑132 (MQ=255)                                                        ccTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCa                                                                                  >  4:234452/1‑149 (MQ=255)                                                          tGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACt                                                                                >  7:268689/1‑149 (MQ=255)                                                          tGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACt                                                                                >  1:314406/1‑149 (MQ=255)                                                             aaGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCAc                                                                                 <  7:214613/145‑1 (MQ=255)                                                             aaGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCAc                                                                                 >  8:214613/1‑145 (MQ=255)                                                                  cGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGAc                                                                        <  3:306615/149‑1 (MQ=255)                                                                    cATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGt                                                                      >  3:189802/1‑149 (MQ=255)                                                                           tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                               >  1:264107/1‑149 (MQ=255)                                                                               gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                           <  8:268689/149‑1 (MQ=255)                                                                               gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                           <  2:264107/149‑1 (MQ=255)                                                                                ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGaa                                                                                                 <  3:117546/110‑1 (MQ=255)                                                                                ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGaa                                                                                                 >  4:117546/1‑110 (MQ=255)                                                                                  aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                        >  4:325747/1‑102 (MQ=255)                                                                                  aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                        <  3:325747/102‑1 (MQ=255)                                                                                    gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgcc                                                      <  1:115513/149‑1 (MQ=255)                                                                                          cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggcgcc                                                <  6:364270/149‑1 (MQ=255)                                                                                          cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggcgcc                                                >  3:124207/1‑149 (MQ=255)                                                                                            cccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGcgccgc                                              >  1:9380/1‑149 (MQ=18)                                                                                             ccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAACTCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                           <  1:72824/136‑1 (MQ=14)                                                                                             ccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                           >  2:72824/1‑135 (MQ=17)                                                                                             ccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCgccgcc                                             >  2:296790/1‑149 (MQ=255)                                                                                               tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACtt                                                                               >  2:91719/1‑112 (MQ=255)                                                                                                   ggcgCGGGTTAACCCGCTAATGCGGAAGTGAATGCACCATCGCTTTCGCGGGTTAACCGGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCt                                        <  2:314406/145‑1 (MQ=255)                                                                                                    gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGa                                      >  7:30824/1‑149 (MQ=255)                                                                                                     aGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                                     <  3:234452/149‑1 (MQ=255)                                                                                                       cGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                   <  4:189802/149‑1 (MQ=255)                                                                                                       cGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                   <  2:9380/149‑1 (MQ=21)                                                                                                         ggTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgc                                 <  1:296790/149‑1 (MQ=255)                                                                                                                ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                           <  5:271300/69‑1 (MQ=255)                                                                                                                ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                           >  6:271300/1‑69 (MQ=255)                                                                                                                 cgcgAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt                         >  5:2797/1‑149 (MQ=25)                                                                                                                  gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt                         >  5:130527/1‑148 (MQ=255)                                                                                                                                gAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggc                                                         <  6:8305/102‑1 (MQ=255)                                                                                                                                gAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggc                                                         >  5:8305/1‑102 (MQ=255)                                                                                                                                        cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCggg  >  7:224460/1‑149 (MQ=255)                                                                                                                                        cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCgg   <  8:224460/148‑1 (MQ=255)                                                                                                                                        cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCACCATATCAAA‑TCTGAAGCTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCCTAGCGGCCGCCggg  >  7:163755/1‑149 (MQ=255)                                                                                                                                         |                                                                                                                                                     CGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGG  >  NC_002947/5643139‑5643422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑