Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,643,290	G→A	7.8%	intergenic (+75/‑48)	dbpA → / → yhiN	23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,643,290	0	G	A	7.8%	187.5 / 2.3	64	intergenic (+75/‑48)	dbpA/yhiN	23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (31/27);  new base A (3/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGTGCTGCT  >  NC_002947/5643146‑5643429                                                                                                                                                 |                                                                                                                                             cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAcc                                                                                                                                          >  3:246180/1‑149 (MQ=255) cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAcc                                                                                                                                          >  6:78107/1‑149 (MQ=255)    gCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                                                                       <  8:231042/149‑1 (MQ=255)            tGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAg                                                                                                                               >  6:319430/1‑149 (MQ=255)               aGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGt                                                                                                                            >  7:259958/1‑149 (MQ=255)                     tGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACACGCGAATGCGGCAGTGAATTCACCATCGCATTCGCGGGTGAACACTCTCCCACAGGGTTctcca                                                                                                                      >  8:297665/1‑145 (MQ=38)                      gAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAt                                                                                                                     >  4:306615/1‑149 (MQ=255)                        aCAGCGGCAAGATCATGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                                                                    >  2:115513/1‑148 (MQ=255)                        aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                         <  8:105732/143‑1 (MQ=255)                        aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                         >  7:105732/1‑143 (MQ=255)                        aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                                   <  5:78107/149‑1 (MQ=255)                         cAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                                  <  8:259958/149‑1 (MQ=255)                             ggCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑t                                                                                                             <  2:69210/149‑1 (MQ=255)                              gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑Tc                                                                                                            >  5:364270/1‑149 (MQ=255)                                  gATCAAGGGTCGCGCCCTGAAAGTACTCATTATCTATAGCCAGGCAAAGCCCCCTGTATTAGCGGGTTTACCCGCTAATGCTTCAGTGAATTCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGaa                                                                                                        <  7:297665/149‑1 (MQ=37)                                   aTCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                                             >  1:63724/1‑128 (MQ=255)                                   aTCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                                             <  2:63724/128‑1 (MQ=255)                                    tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                               >  7:100238/1‑141 (MQ=255)                                    tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                               <  8:100238/141‑1 (MQ=255)                                    tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAgg                                                                                                      <  5:319430/149‑1 (MQ=255)                                      aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGtt                                                                                                    <  4:246180/149‑1 (MQ=255)                                             gcgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                               >  6:280698/1‑132 (MQ=255)                                             gcgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                               <  5:280698/132‑1 (MQ=255)                                                 ccTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCa                                                                                         >  4:234452/1‑149 (MQ=255)                                                   tGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACt                                                                                       >  7:268689/1‑149 (MQ=255)                                                   tGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACt                                                                                       >  1:314406/1‑149 (MQ=255)                                                      aaGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCAc                                                                                        <  7:214613/145‑1 (MQ=255)                                                      aaGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCAc                                                                                        >  8:214613/1‑145 (MQ=255)                                                           cGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGAc                                                                               <  3:306615/149‑1 (MQ=255)                                                             cATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGt                                                                             >  3:189802/1‑149 (MQ=255)                                                                    tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                      >  1:264107/1‑149 (MQ=255)                                                                        gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                  <  8:268689/149‑1 (MQ=255)                                                                        gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                  <  2:264107/149‑1 (MQ=255)                                                                         ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGaa                                                                                                        <  3:117546/110‑1 (MQ=255)                                                                         ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGaa                                                                                                        >  4:117546/1‑110 (MQ=255)                                                                           aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                               >  4:325747/1‑102 (MQ=255)                                                                           aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaaa                                                                                                               <  3:325747/102‑1 (MQ=255)                                                                             gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgcc                                                             <  1:115513/149‑1 (MQ=255)                                                                                   cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggcgcc                                                       <  6:364270/149‑1 (MQ=255)                                                                                   cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggcgcc                                                       >  3:124207/1‑149 (MQ=255)                                                                                     cccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGcgccgc                                                     >  1:9380/1‑149 (MQ=18)                                                                                      ccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAACTCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                  <  1:72824/136‑1 (MQ=14)                                                                                      ccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                  >  2:72824/1‑135 (MQ=17)                                                                                      ccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCgccgcc                                                    >  2:296790/1‑149 (MQ=255)                                                                                        tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACtt                                                                                      >  2:91719/1‑112 (MQ=255)                                                                                            ggcgCGGGTTAACCCGCTAATGCGGAAGTGAATGCACCATCGCTTTCGCGGGTTAACCGGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCt                                               <  2:314406/145‑1 (MQ=255)                                                                                             gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGa                                             >  7:30824/1‑149 (MQ=255)                                                                                              aGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                                            <  3:234452/149‑1 (MQ=255)                                                                                                cGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                          <  4:189802/149‑1 (MQ=255)                                                                                                cGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                          <  2:9380/149‑1 (MQ=21)                                                                                                  ggTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgc                                        <  1:296790/149‑1 (MQ=255)                                                                                                         ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                                  <  5:271300/69‑1 (MQ=255)                                                                                                         ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                                  >  6:271300/1‑69 (MQ=255)                                                                                                          cgcgAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt                                >  5:2797/1‑149 (MQ=25)                                                                                                           gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt                                >  5:130527/1‑148 (MQ=255)                                                                                                                         gAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggc                                                                <  6:8305/102‑1 (MQ=255)                                                                                                                         gAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggc                                                                >  5:8305/1‑102 (MQ=255)                                                                                                                                 cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCggg         >  7:224460/1‑149 (MQ=255)                                                                                                                                 cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCgg          <  8:224460/148‑1 (MQ=255)                                                                                                                                 cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCACCATATCAAA‑TCTGAAGCTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCCTAGCGGCCGCCggg         >  7:163755/1‑149 (MQ=255)                                                                                                                                   ccGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                  >  7:34582/2‑90 (MQ=255)                                                                                                                                   ccGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                  <  8:34582/89‑1 (MQ=255)                                                                                                                                      cATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAg                                  >  5:308152/1‑119 (MQ=255)                                                                                                                                        ttCGCGGGTGAACCCGCTACCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGtgctgct  <  8:163755/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                             CAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAA‑TCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGTGCTGCT  >  NC_002947/5643146‑5643429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑