Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,652,960 T→G 9.7% intergenic (‑61/+100) PP_4961 ← / ← PP_4962 lipoprotein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,652,9600TG 9.7% 133.0 / 8.2 52intergenic (‑61/+100)PP_4961/PP_4962lipoprotein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/27);  new base G (2/3);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01

AGTTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACTTT  >  NC_002947/5652812‑5653105
                                                                                                                                                    |                                                                                                                                                 
aGTTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGt                                                                                                                                                   <  7:177229/149‑1 (MQ=255)
 gTTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTa                                                                                                                                                  <  1:284104/149‑1 (MQ=255)
 gTTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTa                                                                                                                                                  >  5:166914/1‑149 (MQ=255)
    gTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGTTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACa                                                                                                                                                <  1:333831/148‑1 (MQ=255)
         ccGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTa                                                                                                                                                  <  3:55475/141‑1 (MQ=255)
         ccGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTa                                                                                                                                                  >  4:55475/1‑141 (MQ=255)
          cGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGt                                                                                                                                         <  5:263553/149‑1 (MQ=255)
           ggggCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGtc                                                                                                                                        <  3:337641/149‑1 (MQ=255)
                cGGTTGTGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCtgt                                                                                                                                   <  3:269944/149‑1 (MQ=255)
                  gTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTgg                                                                                                                                 >  4:104987/1‑149 (MQ=255)
                      cgcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGc                                                                                                                             <  6:39639/149‑1 (MQ=255)
                       gcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCTGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCa                                                                                                                                           <  2:123852/134‑1 (MQ=255)
                       gcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCGGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCa                                                                                                                                           >  1:123852/1‑134 (MQ=255)
                       gcCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCGCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCg                                                                                                                            >  4:267508/1‑149 (MQ=25)
                        ccGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCgg                                                                                                                           <  8:27666/149‑1 (MQ=255)
                          ggCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGc                                                                                                                         <  3:323120/149‑1 (MQ=25)
                            cTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGa                                                                                                                       <  3:140437/149‑1 (MQ=255)
                              gcagcaGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCATTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGc                                                                                                                     >  8:73026/1‑149 (MQ=255)
                               cagcagTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCGCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGAg                                                                                                                      <  3:267508/147‑1 (MQ=21)
                                  cagTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCtgt                                                                                                                                   >  2:106294/1‑131 (MQ=255)
                                  cagTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCtgt                                                                                                                                   <  1:106294/131‑1 (MQ=255)
                                    gTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGa                                                                                                                       >  3:216815/1‑141 (MQ=21)
                                    gTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGAGACTGCACTGTACCTGTGGGAGCGGGCGa                                                                                                                       <  4:216815/141‑1 (MQ=21)
                                              tGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGcac                                                                                                     >  1:307329/1‑149 (MQ=255)
                                                    tgtCGCCCGGGCCGTCACGCCAAAAAGCGCTTTCATTCAGTTTCCTCTATTTGGGGCTGCTGTCCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGGGGGCGAGCCCGCGAAGGGCCGCACAGCgg                                                                                                <  2:90036/148‑1 (MQ=38)
                                                     gtCGCCAGGGCCGTCACGGCAAAAAGCGCTTTCTTTCCGTTTCCTGGTTTTGGGGCTGCTGTGCCGCCTTTCTCGGGCTCCCCCGCTCCCACAGGTACTGTGCAGTCTGTGTGGGAGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGc                                                                                               <  2:93832/148‑1 (MQ=255)
                                                         ccAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTGCTCGGGCGCGCCCGCTCCCCCCGGTCCAGTGCAGTCTCTGGGGGAGCGGGCGAGCCCGCGAAGGGCCGCTGTGCGGcccttc                                                                                          <  3:28901/149‑4 (MQ=255)
                                                             ggCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGTTTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTGGCCCGATCCCACAGTTACAGTGAATTCTCTGTGGGAGCGGGCTAGCCCGCGAAGGGCCGCACAGCGGCCCcttct                                                                                        <  8:258759/147‑1 (MQ=38)
                                                              gCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACCGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCa                                                                                     <  6:166914/149‑1 (MQ=255)
                                                                 gTCCCGGCCAAACGCGCTTTCATTCTGTTTCCTCTTTTTGGGGCTGCGGGGCAGCCCTTCTCGGGCGCCCCCGCTCCCCCAGTTACTGTGCAGTCTCTGGGGTTGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGa                                                                                  <  2:307329/149‑1 (MQ=255)
                                                                     cGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGc                                                                                                          >  7:141815/1‑121 (MQ=255)
                                                                            aaGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACCGCGGCCCCTTCTTCAAGCCCCCAAAACaa                                                                       >  4:29152/1‑149 (MQ=255)
                                                                                  tttCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGtt                                                                   >  4:150240/1‑147 (MQ=255)
                                                                                          aGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCt                                                         <  3:104987/149‑1 (MQ=255)
                                                                                                        ggggCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAg                                                                                                             >  4:214454/1‑83 (MQ=255)
                                                                                                        ggggCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAg                                                                                                             <  3:214454/83‑1 (MQ=255)
                                                                                                         gggCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAg                                           <  3:150240/148‑1 (MQ=255)
                                                                                                         gggCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAg                                           <  7:73026/148‑1 (MQ=255)
                                                                                                               ctgTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTAtt                                                      <  8:156871/131‑1 (MQ=255)
                                                                                                               ctgTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTAtt                                                      >  7:156871/1‑131 (MQ=255)
                                                                                                                   gctgCCCTTCTCGGGCGCGCCCGCTCCCACTGGTCCTGTCCAGTCGCTGTGGGAGGGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTAt                                                       <  6:291175/123‑1 (MQ=255)
                                                                                                                      gCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGcc                             >  8:272781/1‑149 (MQ=255)
                                                                                                                             tcGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAgg                                          <  3:313124/129‑1 (MQ=255)
                                                                                                                             tcGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAgg                                          >  4:313124/1‑129 (MQ=255)
                                                                                                                              cGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtg                     >  8:144890/1‑149 (MQ=255)
                                                                                                                                      cccGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtggt                   >  4:39739/1‑143 (MQ=255)
                                                                                                                                      cccGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCtggt                   <  3:39739/143‑1 (MQ=255)
                                                                                                                                       ccGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTtc            >  6:316956/1‑149 (MQ=255)
                                                                                                                                          cTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTtcgtc         <  7:272781/149‑1 (MQ=255)
                                                                                                                                              cacaGGTACAGTGCAGTCTCTGTGGGAGCGGGAGAGCCCGCGAAGGGCCGCACAGCGGCCCCGTCTTCAAGACCACAAATAAACGTTCAGGTTGCCTATTAACCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCGTCGGCGTAc     >  8:164638/1‑149 (MQ=255)
                                                                                                                                                 aGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAAc                                                                      >  6:239077/1‑81 (MQ=255)
                                                                                                                                                 aGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAAc                                                                      <  5:239077/81‑1 (MQ=255)
                                                                                                                                                 aGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACttt  >  2:65674/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGTTGTCTGCCGGGGCCGGTTGCGCCGGCTGCAGCAGTGAGCATCCTGCCAGTGTCGCCAGGGCCGTCACGGCCAAAAGCGCTTTCATTCAGTTTCCTCGATTTGGGGCTGCTGTGCAGCCCTTCTCGGGCTCGCCCGCTCCCACAGGTACAGTGCAGTCTCTGTGGGAGCGGGCGAGCCCGCGAAGGGCCGCACAGCGGCCCCTTCTTCAAGACCACAAATCAACGTTCAGGTTGCCTATTACCCGATCAAGGGCAACCCGGCGCCTTGCGCTGGTGGTCTTCGTCGTACTTT  >  NC_002947/5652812‑5653105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑