Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,674,753 G→C 100% intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7530GC100.0% 187.3 / NA 55intergenic (+115/+25)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (28/27);  total (28/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674613‑5674890
                                                                                                                                              |                                                                                                                                          
cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGATCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCgg                                                                                                                                     <  4:344612/149‑1 (MQ=255)
     ctgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTc                                                                                                                                <  7:169952/149‑1 (MQ=255)
        ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGc                                                                                                                             >  5:182500/1‑149 (MQ=255)
         tgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                            >  8:244968/1‑149 (MQ=255)
          gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCAGCTCCTCCAGAGAATTTCTTGATCTCTACAGGACCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCACGATTTTCAGATg                                                                                                                           >  7:58552/1‑149 (MQ=37)
             ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  7:28871/1‑147 (MQ=255)
              cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  8:270426/1‑146 (MQ=255)
                         aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                            <  4:170291/133‑1 (MQ=255)
                          tAGATGTAGGGAGGGGGCTGTTTCTCAGCCCTATCACGACACTATGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCTGTTTTCAGCTGGCATAAATTACTTCt                                                                                                            <  8:58552/148‑1 (MQ=25)
                          tAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTg                                                                                                           <  7:244968/149‑1 (MQ=255)
                             aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAg                                                                                                                       >  7:202911/1‑134 (MQ=255)
                             aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAg                                                                                                                       <  8:202911/134‑1 (MQ=255)
                             aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                        >  6:76686/1‑149 (MQ=255)
                                        gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑a                                                                                            <  8:28871/149‑1 (MQ=255)
                                          gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                              >  8:182170/1‑114 (MQ=255)
                                          gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                              <  7:182170/114‑1 (MQ=255)
                                          gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCACGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTACc‑aca                                                                                          >  8:1166/1‑149 (MQ=37)
                                            tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                             <  4:58415/129‑1 (MQ=255)
                                            tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                             >  3:58415/1‑129 (MQ=255)
                                            tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACt                                                                                        >  2:123538/1‑149 (MQ=255)
                                             gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑a                                                                                            >  1:181251/1‑144 (MQ=255)
                                             gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑a                                                                                            <  2:181251/144‑1 (MQ=255)
                                              ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACt                                                                                        <  7:1166/147‑1 (MQ=255)
                                                    aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGc                                                                                                                             <  1:256523/105‑1 (MQ=255)
                                                       ccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATAtccaggt                                                                             >  6:27751/1‑149 (MQ=255)
                                                           tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATAtccaggtccag                                                                         >  2:238441/1‑149 (MQ=255)
                                                             aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATAtccaggtccagg                                                                        <  7:270426/148‑1 (MQ=255)
                                                             aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATAtccaggtccagg                                                                        <  6:95264/148‑1 (MQ=255)
                                                                     aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGaa                                                               <  5:27751/149‑1 (MQ=255)
                                                                              cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGCACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAc                                                        <  1:238441/148‑1 (MQ=39)
                                                                                 cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTGTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca                                                                                                      >  1:223037/1‑100 (MQ=38)
                                                                                 cAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca                                                                                                      <  2:223037/99‑1 (MQ=255)
                                                                                      aaTTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACt                                                                                        >  2:98412/1‑107 (MQ=255)
                                                                                          tCTTGTTATCTACATGAGCTGCCT‑TGTGTCGTGATAGGTCCTCAAAGCGGCCCCCTCGGGTTTCAGCTGGGAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGTCCTTTGAt                                           <  7:294432/148‑1 (MQ=255)
                                                                                          tCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                           <  3:305167/148‑1 (MQ=255)
                                                                                            ttGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        >  4:129843/1‑149 (MQ=255)
                                                                                                tctcTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                    >  6:316172/1‑149 (MQ=255)
                                                                                                tctcTACAGGAGCGGCCT‑TGTATCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                    >  1:205150/1‑149 (MQ=255)
                                                                                                  tctACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                  >  5:15882/1‑149 (MQ=255)
                                                                                                    tACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTTATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                    <  2:244682/129‑1 (MQ=255)
                                                                                                    tACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                    >  1:244682/1‑129 (MQ=255)
                                                                                                        ggAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGa                                      <  7:27569/139‑1 (MQ=255)
                                                                                                        ggAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGa                                      >  8:27569/1‑139 (MQ=255)
                                                                                                               ccT‑TGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc                     <  1:123538/149‑1 (MQ=255)
                                                                                                                     tatCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt                <  2:205150/147‑1 (MQ=255)
                                                                                                                      gtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg               >  5:214825/1‑149 (MQ=255)
                                                                                                                       tCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                  >  7:145372/1‑145 (MQ=255)
                                                                                                                       tCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                  <  8:145372/145‑1 (MQ=255)
                                                                                                                            aTAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                             >  4:182105/1‑129 (MQ=255)
                                                                                                                            aTAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                             <  3:182105/129‑1 (MQ=255)
                                                                                                                                ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  3:178645/1‑148 (MQ=255)
                                                                                                                                ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  8:13474/1‑148 (MQ=255)
                                                                                                                                  ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  5:361745/1‑146 (MQ=255)
                                                                                                                                  ccGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  6:361745/148‑1 (MQ=255)
                                                                                                                                   cGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  <  3:129843/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                          
CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCT‑TGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG‑ACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674613‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑