Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,062 A→G 8.0% M230V (ATG→GTG)  bioA → adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,0620AG 8.0% 137.8 / 2.7 50M230V (ATG→GTG) bioAadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA
Reads supporting (aligned to +/- strand):  ref base A (20/26);  new base G (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AAGCCCTGACTGCTACCTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCC  >  NC_002947/5678914‑5679204
                                                                                                                                                      |                                                                                                                                              
aaGCCCTGACTGCTACCTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCa                                                                                                                                                <  7:25870/149‑1 (MQ=255)
     cTGACTGCTACCTGCGCCCCGAGGGCATGAGCTGGGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATgcg                                                                                                                                            >  1:52894/1‑149 (MQ=255)
     cTGACTGCTACCTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATgcgc                                                                                                                                           <  3:180767/149‑1 (MQ=255)
     cTGACTGCTACCTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATgcg                                                                                                                                            >  5:173529/1‑148 (MQ=255)
          tGCTACCTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTa                                                                                                                                      >  5:327754/1‑149 (MQ=255)
                cTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCAccc                                                                                                                                <  2:52894/149‑1 (MQ=255)
                 tGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCg                                                                                                                               >  3:130538/1‑149 (MQ=255)
                   cgcCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGgt                                                                                                                             <  6:13446/149‑1 (MQ=255)
                    gcCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGGTACAGGGTGCCGGTGGCGTGCGCATGTACCACCCGgtg                                                                                                                            >  3:310855/1‑149 (MQ=255)
                    gcCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGGTACAGGGTGCCGGTGGCGTGCGCATGTACCACCCGgtg                                                                                                                            >  3:315841/1‑149 (MQ=255)
                     ccccGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGgtg                                                                                                                            >  5:309184/1‑148 (MQ=255)
                      cccGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTa                                                                                                                          >  5:94937/1‑149 (MQ=255)
                       ccGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTAc                                                                                                                         <  4:88169/149‑1 (MQ=255)
                        cGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTAcc                                                                                                                        >  1:325321/1‑149 (MQ=255)
                        cGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTAc                                                                                                                         >  8:131561/1‑148 (MQ=255)
                          aGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTc                                                                                                                      <  7:131561/149‑1 (MQ=255)
                             tcATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGTTACAGGGTGCCGGTGGCGTGCGCATGTACCACCCGGTGTACCTCAAg                                                                                                                   <  4:315841/148‑1 (MQ=255)
                             gCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGGTACAGGGTGCCGGTGGCGTGCGCATGTACCACCCGGTGTACCTCAAg                                                                                                                   <  4:310855/149‑1 (MQ=255)
                                  aGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAgttgtt                                                                                                              <  3:242662/149‑1 (MQ=255)
                                  aGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAgttgt                                                                                                               <  3:9888/148‑1 (MQ=255)
                                     t‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATgc                                                                                                                                             <  4:247093/115‑1 (MQ=255)
                                     t‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTgcg                                                                                                           >  6:117478/1‑149 (MQ=255)
                                                  tCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACCCCACGCCTCGATCAGCGAAGGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGATGTTGCGCGAGGCCTGc                                                                                                 >  1:104458/1‑148 (MQ=255)
                                                    gcgcAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCgg                                                                                                                              <  7:61711/116‑1 (MQ=255)
                                                    gcgcAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCgg                                                                                                                              >  8:61711/1‑116 (MQ=255)
                                                    gcgcAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGAc                                                                                              <  6:327754/148‑1 (MQ=255)
                                                     cgcAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCg                                                                                            <  4:130538/149‑1 (MQ=255)
                                                      gcAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGc                                                                                           <  4:8782/149‑1 (MQ=255)
                                                        aaCATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAgttgt                                                                                                               >  1:342229/1‑127 (MQ=255)
                                                        aaCATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAgttgt                                                                                                               <  2:342229/127‑1 (MQ=255)
                                                         aCATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTAt                                                                                        <  5:56383/149‑1 (MQ=255)
                                                              ttCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGAc                                                                                              <  4:8603/138‑1 (MQ=255)
                                                                 cAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCa                                                                                <  6:173529/149‑1 (MQ=255)
                                                                   ggCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGGCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCAcc                                                                              <  5:117478/149‑1 (MQ=255)
                                                                          gAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATcc                                                                        <  2:104458/148‑1 (MQ=255)
                                                                              aGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCacgac                                                                   <  6:309184/149‑1 (MQ=255)
                                                                                           acacCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTgg                                                      <  7:26108/149‑1 (MQ=255)
                                                                                                acGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTc                                                 >  5:217929/1‑149 (MQ=255)
                                                                                                   ccTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGc                                              <  6:94937/149‑1 (MQ=255)
                                                                                                    cTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGc                                                                                                 <  1:256221/97‑1 (MQ=255)
                                                                                                    cTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGc                                                                                                 >  2:256221/1‑97 (MQ=255)
                                                                                                    cTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGcc                                             >  3:55526/1‑149 (MQ=255)
                                                                                                      cGATCAGCG‑CCGTGATCATCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCg                                            >  3:304585/1‑148 (MQ=255)
                                                                                                                   tGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATg                               <  4:55526/149‑1 (MQ=255)
                                                                                                                      tcgtcgAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTc                            <  2:325321/149‑1 (MQ=255)
                                                                                                                           gAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTg                       >  2:289963/1‑149 (MQ=255)
                                                                                                                                        aGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCgg           >  6:100998/1‑148 (MQ=255)
                                                                                                                                               cGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGcc   >  4:211293/1‑149 (MQ=255)
                                                                                                                                               cGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGACCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGcc   >  3:42127/1‑149 (MQ=255)
                                                                                                                                                ggTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc  <  5:100998/149‑1 (MQ=255)
                                                                                                                                                ggTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGccc  <  3:211293/149‑1 (MQ=255)
                                                                                                                                                      aTGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTg                                                       >  7:306936/1‑90 (MQ=255)
                                                                                                                                                      aTGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTg                                                       <  8:306936/90‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                              
AAGCCCTGACTGCTACCTGCGCCCCGAGGGCATGAGCT‑GGGAGGAGCACTCGCGCAACATGTTCCAGGCCATGGAGCAGACCCTGGCCGAACACCACGCCTCGATCAGCG‑CCGTGATCGTCGAGCCGCTGATACAGGGTGCCGGTGGCATGCGCATGTACCACCCGGTGTACCTCAAGTTGTTGCGCGAGGCCTGCGACCGCTATGACGTGCACCTTATCCACGACGAGATCGCCGTGGGCTTCGGCCGCACCGGCACGATGTTCGCCTGCGAGCAAGCCGGCATCCGCCC  >  NC_002947/5678914‑5679204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑