Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,683,859 T→A 5.8% T1012S (ACA→TCA) ‡ PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,683,8590TA 5.8% 252.6 / 2.7 82T1012S (ACA→TCA) ‡PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base T (38/39);  new base A (2/3);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TCGAGTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACTTGGCGG  >  NC_002947/5683715‑5684003
                                                                                                                                                |                                                                                                                                                 
tCGAGTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGt                                                                                                                                               <  8:241982/149‑1 (MQ=255)
    gTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCgg                                                                                                                                           >  4:61304/1‑149 (MQ=255)
       cTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCCGCACTGACAGACAGttccgcg                                                                                                                                         <  7:86164/148‑7 (MQ=255)
        tCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCCCGGACAGACAGTGCCGcgag                                                                                                                                       >  3:52675/1‑146 (MQ=255)
        tCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCAct                                                                                                                                       >  8:9355/1‑149 (MQ=255)
        tCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCAct                                                                                                                                       >  8:244577/1‑149 (MQ=255)
        tCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGACAGACAGTGCCGc                                                                                                                                          <  4:52675/146‑1 (MQ=255)
             tGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCActgctg                                                                                                                                   >  7:163321/1‑148 (MQ=255)
               ccAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGaa                                                                                                                                 <  8:332505/148‑1 (MQ=255)
               ccAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGa                                                                                                                                  <  8:175552/147‑1 (MQ=255)
               ccAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAg                                                                                                                                >  3:241835/1‑149 (MQ=255)
                 aGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGtt                                                                                                                              >  4:284414/1‑149 (MQ=255)
                   gTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGc                                                                                                                            <  8:163321/149‑1 (MQ=255)
                        ccAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATg                                                                                                                       >  5:231461/1‑149 (MQ=255)
                        ccAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATg                                                                                                                       >  7:14003/1‑149 (MQ=255)
                        ccAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATg                                                                                                                       >  5:236373/1‑149 (MQ=255)
                        ccAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATg                                                                                                                       <  4:241835/149‑1 (MQ=255)
                         cAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGt                                                                                                                      <  3:61304/149‑1 (MQ=255)
                          aGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTa                                                                                                                     <  7:164811/149‑1 (MQ=255)
                                    gcagcTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCAct                                                                                                                                       <  4:316286/121‑1 (MQ=255)
                                    gcagcTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCAct                                                                                                                                       >  3:316286/1‑121 (MQ=255)
                                    gcagcTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAgc                                                                                                           <  7:244577/149‑1 (MQ=255)
                                       gcTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCt                                                                                                        <  6:231461/149‑1 (MQ=255)
                                          cGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTg                                                                                                       >  3:251141/1‑147 (MQ=255)
                                          cGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTg                                                                                                       >  2:60038/1‑147 (MQ=255)
                                            gCGTCGACCTTGACCATGTAACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCACCTGGCCCGCGGCACTGTCTGTCAGTGCGGGAGTGCTGATGTTGCGAATGTAGTCGATCAGCGCTGTAGc                                                                                                   >  7:230528/1‑149 (MQ=255)
                                                 gACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAgcg                                                                                                          >  1:310852/1‑137 (MQ=255)
                                                 gACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAgcg                                                                                                          <  2:310852/137‑1 (MQ=255)
                                                 gACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCAt                                                                                              <  8:137659/149‑1 (MQ=255)
                                                 gACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCAt                                                                                              >  6:139134/1‑149 (MQ=255)
                                                 gACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCAt                                                                                              >  7:137659/1‑149 (MQ=255)
                                                  aCCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAAt                                                                                                                        <  6:147940/122‑1 (MQ=255)
                                                  aCCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGACATg                                                                                             <  4:251141/149‑1 (MQ=255)
                                                   ccTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATg                                                                                             >  1:254798/1‑148 (MQ=255)
                                                   ccTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATg                                                                                             >  3:128331/1‑148 (MQ=255)
                                                    cTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATg                                                                                             >  5:227163/1‑147 (MQ=255)
                                                    cTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCCGCACTGACAGACAGTGCCGcgg                                                                                                                                        >  1:242165/1‑102 (MQ=255)
                                                    cTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCCGCACTGACAGACAGTGCCGcgg                                                                                                                                        <  2:242165/104‑3 (MQ=255)
                                                     ttGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAg                                                                                          >  4:242420/1‑149 (MQ=255)
                                                       gACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCg                                                                                        >  3:32365/1‑149 (MQ=255)
                                                         ccATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGc                                                                                      >  7:153639/1‑149 (MQ=255)
                                                                aCCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATg                                                                                             <  2:144664/135‑1 (MQ=255)
                                                                aCCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATg                                                                                             >  1:144664/1‑135 (MQ=255)
                                                                aCCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGccc                                                                                 >  3:183194/1‑147 (MQ=255)
                                                                aCCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGccc                                                                                 >  6:93270/1‑147 (MQ=255)
                                                                  ccGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGccc                                                                                 >  8:184821/1‑145 (MQ=255)
                                                                  ccGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGccc                                                                                 <  7:184821/145‑1 (MQ=255)
                                                                        cccGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGcact                                                                       <  2:254798/149‑2 (MQ=255)
                                                                        cccGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGcaca                                                                       <  3:242420/149‑1 (MQ=255)
                                                                        cccGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGcaca                                                                       <  4:128331/149‑1 (MQ=255)
                                                                         ccGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTAGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAg                                                                      >  8:50356/1‑149 (MQ=255)
                                                                                 gcgcTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCaa                                                                <  3:284414/147‑1 (MQ=255)
                                                                                 gcgcTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCaa                                                                <  6:236373/147‑1 (MQ=255)
                                                                                 gcgcTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAg                                                               >  5:193752/1‑148 (MQ=255)
                                                                                 gcgcTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGc                                                              <  6:193752/149‑1 (MQ=255)
                                                                                 gcgcTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGc                                                              <  4:183194/149‑1 (MQ=255)
                                                                                 gcgcTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGc                                                              <  1:60038/149‑1 (MQ=255)
                                                                                   gctgctGCCGGTATTTCTGCTGTGGCAGCTTCTGGCGGTCCCTGGCCAGCGGAACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCat                                                            <  8:67320/149‑1 (MQ=255)
                                                                                   gctgctGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCat                                                            <  5:139134/149‑1 (MQ=255)
                                                                                       ctgcCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑Tg                                                        <  8:14003/148‑1 (MQ=255)
                                                                                          ccGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGcc                                                    <  8:153639/149‑1 (MQ=255)
                                                                                             gTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGc                                                 >  2:325918/1‑149 (MQ=255)
                                                                                                tttCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCgg                                              <  5:89011/149‑1 (MQ=255)
                                                                                                tttCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCgg                                              <  7:9355/149‑1 (MQ=255)
                                                                                                          ggCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTgg                                        <  6:5309/145‑1 (MQ=255)
                                                                                                          ggCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTgg                                        >  5:5309/1‑145 (MQ=255)
                                                                                                                   tGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTgcag                             >  4:33907/1‑147 (MQ=255)
                                                                                                                     gcggcCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTgcagca                           <  6:227163/147‑1 (MQ=255)
                                                                                                                         ccccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCg                      >  2:91697/1‑148 (MQ=255)
                                                                                                                           ccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTTGTGGCAGTTCTGCAGCAGt                         <  1:128411/143‑1 (MQ=255)
                                                                                                                           ccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTTGTGGCAGTTCTGCAGCAGt                         >  2:128411/1‑143 (MQ=255)
                                                                                                                           ccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGaa                    <  7:66448/148‑1 (MQ=255)
                                                                                                                           ccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGaa                    >  8:66448/1‑148 (MQ=255)
                                                                                                                           ccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGaa                    >  7:3117/1‑148 (MQ=255)
                                                                                                                           ccTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAAc                   <  5:93270/149‑1 (MQ=255)
                                                                                                                               gCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTg               <  3:33907/149‑1 (MQ=255)
                                                                                                                                 ccGCTGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGAGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGgc             <  8:230528/149‑1 (MQ=255)
                                                                                                                                 ccGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGc                                                                                                <  4:69421/67‑1 (MQ=255)
                                                                                                                                 ccGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGc                                                                                                >  3:69421/1‑67 (MQ=255)
                                                                                                                                 ccGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGgc             >  7:315776/1‑149 (MQ=255)
                                                                                                                                       cACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACtt       <  8:315776/149‑1 (MQ=255)
                                                                                                                                        aCTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACTTg      >  1:201905/1‑149 (MQ=255)
                                                                                                                                          tgtctgtcAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACTTggc    >  4:127629/1‑149 (MQ=255)
                                                                                                                                            tctgtcAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACTTggcgg  >  6:115300/1‑149 (MQ=255)
                                                                                                                                            tctgtcAGTGCGGCACTGCTGAAGTTGCGAATGTAGGCGCTCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATACTGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACTTgg     <  1:91697/147‑1 (MQ=255)
                                                                                                                                                tcAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCg                      >  8:134928/1‑125 (MQ=255)
                                                                                                                                                tcAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCg                      <  7:134928/125‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCGAGTGCTCGCCTGCCAGGTTACCCAGGTCGTCGAGCAGCTCGGCGTCGACCTTGACCATGTCACCCGGTGCCCGCTCTGGCGCTGCTGCCGGTATTTCGGCGGTGGCAGCTTCTGGCGGCCCCTGGCCCGCGGCACTGTCTGTCAGTGCGGCACTGCTGAAGTTGCGAATGTAGTCGATCAGCGCTGTAGCGGCATGCAGCGGCTGCCCCAGACGCACAGCGTCAAGCATA‑TGCGCCAGCCGGTCGTGGCAGTTCTGCAGCAGTGCGAACAGTGGCGCACTTGGCGG  >  NC_002947/5683715‑5684003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑