Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,692,563 C→T 9.2% intergenic (+32/‑138) PP_4994 → / → PP_4995 hypothetical protein/exopolysaccharide biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,692,5630CT 9.2% 150.8 / 4.3 54intergenic (+32/‑138)PP_4994/PP_4995hypothetical protein/exopolysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (26/23);  new base T (3/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GCGCCGTTTGCACCGTTTACCGGGGATCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACTATCCCCCATGAAA  >  NC_002947/5692430‑5692706
                                                                                                                                     |                                                                                                                                               
gcgcCGTTTGCACCGTTTACCGGGGATCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACggg                                                                                                                                  >  5:339907/1‑149 (MQ=255)
  gcCGTTTGCACCGTTTACCGGGGATCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGtt                                                                                                                                <  2:269293/149‑1 (MQ=255)
              gTTTACCGGGGATCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGgcgc                                                                                                                    >  7:259735/1‑149 (MQ=255)
                   ccGGGGATCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGaa                                                                                                                <  6:339907/148‑1 (MQ=255)
                         aTCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGc                                                                                                              <  4:180809/144‑1 (MQ=255)
                         aTCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGc                                                                                                              >  3:180809/1‑144 (MQ=255)
                             aGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAg                                                                                                      >  8:69586/1‑148 (MQ=255)
                              gCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCtgtg                                                                                                         <  1:80998/144‑1 (MQ=255)
                              gCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGa                                                                                                       <  3:315822/146‑1 (MQ=255)
                              gCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCAAGTGCCGGCCTCTTCGCGGGCGTGCCCGcgaag                                                                                                                                       <  4:47623/114‑5 (MQ=39)
                              gCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCAAGTGCCGGCCTCTTCGCGGGCGTGCCCGcgaag                                                                                                                                       >  3:47623/1‑110 (MQ=39)
                                        aCCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCt                                                                                                                   >  3:215068/1‑124 (MQ=255)
                                        aCCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCt                                                                                                                   <  4:215068/124‑1 (MQ=255)
                                           ggCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCgg                                                                                       <  8:259735/149‑1 (MQ=255)
                                             ctcgagaTCATCCGCACCTGGCGCTTTCCCCGTGGGGACCGATTGTCCAGTAACTGATTTTCTGCAAGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTAGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGc                                                                                     <  5:279117/146‑1 (MQ=39)
                                                  gaTCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGcc                                                                                >  6:358124/1‑149 (MQ=255)
                                                     catcCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGaa                                                                                                                >  5:358219/1‑114 (MQ=255)
                                                     catcCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGaa                                                                                                                <  6:358219/114‑1 (MQ=255)
                                                       tcCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCcgcg                                                                            >  2:105213/1‑148 (MQ=255)
                                                        ccGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGACGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCggcg                                                                            >  4:270849/1‑147 (MQ=255)
                                                              cTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCt                                                                    >  7:73107/1‑149 (MQ=255)
                                                                 gcgcTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTgg                                                                  <  5:358124/148‑1 (MQ=255)
                                                                   gcTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAg                                                                         >  6:357109/1‑139 (MQ=255)
                                                                       tGCCCGTGGGGACCGGTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCaaaa                                                             <  5:284740/147‑1 (MQ=255)
                                                                          ccGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGa                                                                                                       >  2:237564/1‑102 (MQ=255)
                                                                          ccGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGc                                                           <  6:360649/146‑1 (MQ=255)
                                                                          ccGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGc                                                           >  5:360649/1‑146 (MQ=255)
                                                                            gTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGaca                                                       >  7:157968/1‑148 (MQ=255)
                                                                                   ccGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGccc                                               <  7:69586/149‑1 (MQ=255)
                                                                                     gTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCtgtg                                                                                                         >  4:18664/1‑89 (MQ=255)
                                                                                         gTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTg                                         >  2:279682/1‑149 (MQ=255)
                                                                                           catttAACTGATTTTCTGCTTGTGCCGGCCTCTTCGCGGGCATGCCCGCTCCCAGGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGTGGGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGt                                        <  8:73107/144‑1 (MQ=39)
                                                                                            cAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGt                                        >  1:264808/1‑147 (MQ=255)
                                                                                            cAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGt                                        <  2:264808/147‑1 (MQ=255)
                                                                                            cAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGt                                        >  3:263461/1‑147 (MQ=255)
                                                                                            cAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTc                                       <  1:279682/148‑1 (MQ=255)
                                                                                            cAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCa                                      <  1:105213/149‑1 (MQ=255)
                                                                                                   tGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACCCCCCCTCCCACGGGTTGCTCACCGGCGCTGAAGCCAGTGGAGTATAGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAACGACACAAAGCCCACCATGTCAGtcaacc                               >  8:311780/1‑145 (MQ=255)
                                                                                                       tttCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGt                                        >  6:192888/1‑136 (MQ=255)
                                                                                                       tttCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGt                                        <  5:192888/136‑1 (MQ=255)
                                                                                                              aTGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGcc                    >  7:180311/1‑149 (MQ=255)
                                                                                                                gtgCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTTCTAACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCCGGCATGCCCCCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCATGTCACTCAAGCCACCAGGCGCcac                  >  4:243334/1‑149 (MQ=38)
                                                                                                                 tgCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCcac                  <  4:263461/148‑1 (MQ=255)
                                                                                                                   ccGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGa                                                         <  4:243597/107‑1 (MQ=255)
                                                                                                                   ccGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGa                                                         >  3:243597/1‑107 (MQ=255)
                                                                                                                   ccGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTAGCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACt               <  3:243334/149‑1 (MQ=255)
                                                                                                                      gCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCt                          >  5:321142/1‑135 (MQ=255)
                                                                                                                      gCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCt                          <  6:321142/135‑1 (MQ=255)
                                                                                                                         tctTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGtctc                                 >  3:303939/1‑125 (MQ=255)
                                                                                                                         tctTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGGGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGtctc                                 <  4:303939/125‑1 (MQ=255)
                                                                                                                          ctTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACTATcccc         >  5:111092/1‑148 (MQ=255)
                                                                                                                           ttCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACTATCCCCCa       >  1:328628/1‑149 (MQ=255)
                                                                                                                           ggagcgGGCGTGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACTATCCCCCa       >  3:34638/4‑149 (MQ=255)
                                                                                                                                gggCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACTATCCCCCATGaaa  <  8:180311/149‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                               
GCGCCGTTTGCACCGTTTACCGGGGATCTAGCCGAGTTCGACCGGCTGGAGATCATCCGCACCTGGCGCTTTGCCCGTGGGGACCGTTTGTCCAGTAACTGATTTTCTGCATGTGCCGGCCTCTTCGCGGGCACGCCCGCTCCCACGGGTTGCTCACCGGCGCTGAAGCCTGTGGAGTATCGGCGGGAGCGGGCATGCCCGCGAAGGGGCTGGCAAAAGCGACACAAAGCCCACCTTGTCAGTCTCGCCACCTGGCGCCACACTATCCCCCATGAAA  >  NC_002947/5692430‑5692706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑