Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,694,013 T→A 16.3% L90Q (CTG→CAG)  pyrR → pyrimidine operon regulatory protein/uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,694,0130TA16.3% 111.2 / 15.0 49L90Q (CTG→CAG) pyrRpyrimidine operon regulatory protein/uracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (22/19);  new base A (5/3);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

TTGCCCAGGCCCTGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGCCGATTCGCCCGAACGTGCT  >  NC_002947/5693866‑5694160
                                                                                                                                                   |                                                                                                                                                   
ttGCCCAGGCCCTGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctg                                                                                                                                                    <  7:55645/149‑1 (MQ=255)
   cccAGGCCCTGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctggtg                                                                                                                                                 >  3:154468/1‑149 (MQ=255)
   cccAGGCCCTGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACCGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctgctg                                                                                                                                                 >  5:337346/1‑149 (MQ=255)
        gCCCTGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctggtgctggt                                                                                                                                            <  6:90695/149‑1 (MQ=255)
            tGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGAt                                                                                                                                        >  3:131785/1‑149 (MQ=255)
             gCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctggtgctggt                                                                                                                                            >  3:333304/1‑144 (MQ=255)
             gCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctggtgctggt                                                                                                                                            <  4:333304/144‑1 (MQ=255)
              cAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATg                                                                                                                                       <  4:131785/148‑1 (MQ=255)
                 gATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGt                                                                                                                                   >  4:89163/1‑149 (MQ=255)
                    gCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGc                                                                                                                                 <  4:154468/148‑1 (MQ=255)
                     ccATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCt                                                                                                                                >  6:200477/1‑148 (MQ=255)
                       aTGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCtgat                                                                                                                             >  6:345934/1‑149 (MQ=255)
                          ggCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAg                                                                                                                          <  5:200477/149‑1 (MQ=255)
                            cGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCg                                                                                                                        <  5:345934/149‑1 (MQ=255)
                                acCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCg                                                                                                                    >  5:140009/1‑149 (MQ=255)
                                  cAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCtgatga                                                                                                                           >  7:202221/1‑140 (MQ=255)
                                  cAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCtgatga                                                                                                                           <  8:202221/140‑1 (MQ=255)
                                  cAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCa                                                                                                                  >  6:153510/1‑149 (MQ=255)
                                  cAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCa                                                                                                                  <  3:89163/149‑1 (MQ=255)
                                            ggCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctggtgctg                                                                                                                                              <  6:244701/111‑1 (MQ=255)
                                            ggCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACctggtgctg                                                                                                                                              >  5:244701/1‑111 (MQ=255)
                                                 ccTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCAc                                                                                                                 <  3:138465/135‑1 (MQ=255)
                                                 ccTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCAc                                                                                                                 >  4:138465/1‑135 (MQ=255)
                                                        gtgtCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACg                                                                                             >  2:273668/1‑148 (MQ=255)
                                                         tgtCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGaa                                                                                           <  5:153510/149‑1 (MQ=255)
                                                           tCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACt                                                                                         >  8:197446/1‑149 (MQ=255)
                                                             gTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCgcgg                                                                                                      >  5:50287/1‑134 (MQ=255)
                                                             gTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCgcgg                                                                                                      <  6:50287/134‑1 (MQ=255)
                                                                cTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTc                                                                                     <  7:197446/148‑1 (MQ=255)
                                                                   ccGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCg                                                                                    <  1:273668/146‑1 (MQ=255)
                                                                      cgacgaCTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCg                                                                                    >  3:12648/1‑143 (MQ=255)
                                                                      cgacgaCTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCg                                                                                    <  4:12648/143‑1 (MQ=255)
                                                                      cgacgaCTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACg                                                                              >  2:37803/1‑149 (MQ=255)
                                                                                  ccAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGc                                                                    >  6:151142/1‑147 (MQ=255)
                                                                                    aGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACg                                                                              >  4:327024/1‑135 (MQ=255)
                                                                                    aGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACg                                                                              <  3:327024/135‑1 (MQ=255)
                                                                                    aGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGc                                                                <  5:151142/149‑1 (MQ=255)
                                                                                                    acaAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTctgcc                                                <  1:37803/149‑1 (MQ=255)
                                                                                                                gTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTg                                     >  3:9192/1‑148 (MQ=255)
                                                                                                                 tCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGa                                   >  6:1083/1‑149 (MQ=255)
                                                                                                                           cGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGaa                         >  7:73662/1‑149 (MQ=255)
                                                                                                                                  ggTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCctgct                                            >  2:329248/1‑123 (MQ=255)
                                                                                                                                      gAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGAcc                                       >  2:289192/1‑124 (MQ=255)
                                                                                                                                      gAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGAcc                                       <  1:289192/124‑1 (MQ=255)
                                                                                                                                        ccaccAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGAt                                  >  6:15481/4‑127 (MQ=255)
                                                                                                                                        ccaccAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGAt                                  <  5:15481/124‑1 (MQ=255)
                                                                                                                                         caccAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGCCGATTCGccc           >  6:167357/3‑149 (MQ=255)
                                                                                                                                         caccAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGCCGATTCGccc           >  6:162274/3‑149 (MQ=255)
                                                                                                                                          accAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGc                     >  3:17012/2‑138 (MQ=255)
                                                                                                                                          accAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGc                     <  4:17012/137‑1 (MQ=255)
                                                                                                                                           ccAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTg                                     <  3:117466/121‑1 (MQ=255)
                                                                                                                                           ccAGCACCAGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTg                                     >  4:117466/1‑121 (MQ=255)
                                                                                                                                            cAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCgg                             >  2:23658/1‑128 (MQ=255)
                                                                                                                                            cAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCgg                             <  1:23658/128‑1 (MQ=255)
                                                                                                                                             aGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTctgcctgc                                             >  6:220058/1‑111 (MQ=255)
                                                                                                                                             aGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTctgcctgc                                             <  5:220058/111‑1 (MQ=255)
                                                                                                                                             aGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGCCGATTCGCCCGAAc       <  6:140009/149‑1 (MQ=255)
                                                                                                                                                  ctggtgctggtgGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGCCGATTCGCCCGAACGTGCt  >  7:329440/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGCCCAGGCCCTGCAGGATGCCATGGGCGACACCAGCCCAATGGGCACCCTGGACGTGTCGTTCTACCGCGACGACTTCAGCCAGAACGGCCTGCACCCACAAGTGCGCCCGTCCGAGTTGCCGTTCGAGGTCGAAGGCCAGCACCTGGTGCTGGTGGATGACGTGCTGATGAGCGGTCGCACCATCCGCGCGGCGCTCAACGAACTGTTCGATTACGGCCGCCCGGCCAGCGTCACCCTGGTCTGCCTGCTGGACCTGGATGCCGGCGAACTGCCGATTCGCCCGAACGTGCT  >  NC_002947/5693866‑5694160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑