| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,740,467 | T→G | 16.4% | intergenic (+32/+39) | hutF → / ← PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,740,467 | 0 | T | G | 16.4% | 56.3 / 5.1 | 30 | intergenic (+32/+39) | hutF/PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (9/16); new base G (3/2); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
CCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCA > NC_002947/5740321‑5740607 | ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGc < 6:308134/149‑1 (MQ=255) tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGCGAGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg > 4:37684/1‑149 (MQ=255) tgtgATGGTGAATCGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAg < 3:121033/149‑1 (MQ=39) aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt < 5:280021/149‑1 (MQ=255) aTGGTGAATGGGCAATGGGGGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGACAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGGTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt < 3:37684/149‑1 (MQ=255) ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAGCCCGTAGCGGGGAACCCCCCCCAGGGtttg > 2:9587/1‑147 (MQ=255) ggTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCCTTGCAGCCCTTAGCGTGCAAGCGCGCGAAGGGTTg > 7:234789/1‑148 (MQ=255) tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAggg < 4:119943/143‑1 (MQ=255) tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAggg > 3:119943/1‑143 (MQ=255) tggtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTAGCGTGCAAACCCGCGAAAGGTTGCAACGCAACCCCcaaaa > 4:223382/1‑148 (MQ=255) ggggcgcCTGGGGCGGATGGCCCAGTACTTTTAACGGGGGCGGGCGTTGGCAACGGTGTGGCGCCAGCTTTTGGGCTGCGCTTACCGGGGTTGCGTTCCAGCCCTCCGCGTGCAAGCCCGCTATGGGTTGAAACGCAACCCCAAAAAAc < 8:134779/146‑1 (MQ=255) aaGATGTATGCGGGCGGGTGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTTAGCTTACCGGGGTTGGTTTTCATCCCTTCGCGTGCAATCCCGGTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGAt < 3:223382/149‑1 (MQ=255) aTGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCTTAGCGGGGAAGCCCGCGCAGGGTTGGCACGCCACCCCCAAAAACTCAGTGGCTTTTTACTATTTGCTGATCgg > 7:305590/1‑149 (MQ=255) ccgggcgggCGTTTGCCACGGTGGTCCGCCCCGTTTTGGGGGGAGCCTACCGGGGGTGCGTGGCAGCCTTCCGCGTGCACCCCCGCTACGGGTTCAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgccc < 6:8842/148‑1 (MQ=255) cgggcgTTTGCCATGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGc < 3:253955/131‑1 (MQ=255) ttGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgt < 5:148451/145‑1 (MQ=255) ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgc < 6:67562/68‑1 (MQ=255) ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgc < 4:276327/68‑1 (MQ=255) ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgc > 5:67562/1‑68 (MQ=255) ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgc > 3:276327/1‑68 (MQ=255) ttttGGGGTGTGCTTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGATGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct < 1:143820/149‑1 (MQ=255) ggAGCGTACCGGGGTTGCGTTGCAGCCCTTCGGGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc < 8:234789/148‑1 (MQ=255) aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTCGTGTCATACCCCTGCTGCCGCGCCCt < 1:9587/149‑1 (MQ=255) gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt > 6:33480/1‑149 (MQ=255) ttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGGAACGCAACCCCAAAAAACTCAGTGCa > 5:328849/1‑67 (MQ=255) ttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCa < 6:328849/67‑1 (MQ=255) gttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAg < 7:2173/101‑1 (MQ=21) gttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAg > 8:2173/1‑101 (MQ=21) gCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc > 7:215837/1‑149 (MQ=255) ccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCa < 8:215837/149‑1 (MQ=255) | CCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCA > NC_002947/5740321‑5740607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |