Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,741,895	A→G	6.8%	F87L (TTC→CTC)	PP_5039 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,741,895	0	A	G	6.8%	208.1 / 4.6	73	F87L (TTC→CTC)	PP_5039	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (35/33);  new base G (3/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

GCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATG  >  NC_002947/5741748‑5742036                                                                                                                                                    |                                                                                                                                              gCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAg                                                                                                                                              >  6:27093/1‑149 (MQ=255)   tCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGGAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGACACGCCGCGCGCCTTCGAGGgc                                                                                                                                            >  1:111458/1‑149 (MQ=255)   tCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgc                                                                                                                                            >  3:182316/1‑149 (MQ=255)       cGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgcgc                                                                                                                                        <  5:27093/149‑1 (MQ=255)         cacaGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGACACGCCGCGCGCCTTCGAGGgcgaccgg                                                                                                                                      <  2:111458/149‑6 (MQ=255)              gTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCgtgt                                                                                                                                 >  8:78905/1‑149 (MQ=255)              gTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCgtgt                                                                                                                                 >  2:124269/1‑149 (MQ=255)                    ccAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCgg                                                                                                                            <  4:68258/148‑1 (MQ=255)                     cAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTa                                                                                                                          <  2:230289/149‑1 (MQ=255)                       gTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGa                                                                                                                        <  7:262863/149‑1 (MQ=255)                        tCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCgg                                                                                                                            >  2:233791/1‑144 (MQ=255)                        tCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCCAAGGCGCGCGGCGTGTCGCgg                                                                                                                            >  5:7546/1‑144 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgcgc                                                                                                                                        <  7:250055/130‑1 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgc                                                                                                                                          >  8:65750/1‑128 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGgcgc                                                                                                                                          <  7:65750/128‑1 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCa                                                                                                                     >  8:128694/1‑149 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCa                                                                                                                     >  5:323790/1‑149 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCa                                                                                                                     >  3:11800/1‑149 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCa                                                                                                                     >  2:181142/1‑149 (MQ=255)                          cAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCa                                                                                                                     >  8:16600/1‑149 (MQ=255)                               cTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGt                                                                                                                <  7:128694/149‑1 (MQ=255)                                    gCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAg                                                                                                           >  6:358515/1‑149 (MQ=255)                                          tCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTgg                                                                                                              >  8:296146/1‑140 (MQ=255)                                          tCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTgg                                                                                                              <  7:296146/140‑1 (MQ=255)                                          tCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTc                                                                                                     >  4:334420/1‑149 (MQ=255)                                            cAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTc                                                                                                     <  7:16600/147‑1 (MQ=255)                                            cAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTc                                                                                                     <  6:311831/147‑1 (MQ=255)                                                      agcaCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcag                                                                                         <  4:182316/149‑1 (MQ=255)                                                        caCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAg                                                                                                           >  4:311724/1‑129 (MQ=255)                                                        caCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAg                                                                                                           <  3:311724/129‑1 (MQ=255)                                                        caCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGa                                                                                                   <  5:363028/137‑1 (MQ=255)                                                        caCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGa                                                                                                   >  6:363028/1‑137 (MQ=255)                                                        caCGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcagca                                                                                       <  7:78905/149‑1 (MQ=255)                                                              cacGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGcc                                                                                   >  3:58080/1‑147 (MQ=255)                                                                   gcTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCccagccag                                                                            <  4:11800/149‑1 (MQ=255)                                                                       gATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGccc                                                                         <  1:181142/148‑1 (MQ=255)                                                                            gcagcaGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcag                                                                                         >  6:170291/1‑127 (MQ=255)                                                                            gcagcaGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATcag                                                                                         <  5:170291/127‑1 (MQ=255)                                                                            gcagcaGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCggg                                                                   >  6:252503/1‑149 (MQ=255)                                                                                 agcTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTtg                                                              <  5:358515/149‑1 (MQ=255)                                                                                      gTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTtgct                                                            <  8:242685/146‑1 (MQ=255)                                                                                        aGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGa                                                                                                   >  1:75030/1‑105 (MQ=255)                                                                                        aGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGa                                                                                                   <  2:75030/105‑1 (MQ=255)                                                                                        aGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCAc                                                       >  4:214768/1‑149 (MQ=255)                                                                                            ccAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCActct                                                    >  5:33200/1‑148 (MQ=255)                                                                                             cAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTc                                                  >  3:24445/1‑149 (MQ=255)                                                                                               gCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGa                                                <  6:7546/149‑1 (MQ=255)                                                                                               gCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGa                                                <  3:334420/149‑1 (MQ=255)                                                                                                     cTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCa                                           <  1:124269/148‑1 (MQ=255)                                                                                                     cTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCa                                           <  8:215332/148‑1 (MQ=255)                                                                                                        cAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCg                                       >  1:50731/1‑149 (MQ=255)                                                                                                              cTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCa                                    <  8:23168/146‑1 (MQ=255)                                                                                                              cTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCa                                    >  7:23168/1‑146 (MQ=255)                                                                                                               tGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGc                                >  1:269531/1‑149 (MQ=255)                                                                                                                 cATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGt                              <  2:50731/149‑1 (MQ=255)                                                                                                                          caccTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCggg                                                                   >  6:215499/1‑103 (MQ=255)                                                                                                                            ccTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTgg                                                                                                       <  3:25309/65‑1 (MQ=255)                                                                                                                            ccTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTgg                                                                                                       >  4:25309/1‑65 (MQ=255)                                                                                                                             cTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTc                          <  4:197597/141‑1 (MQ=255)                                                                                                                             cTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTc                          >  3:197597/1‑141 (MQ=255)                                                                                                                                ggCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTTTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTc                 <  1:233791/147‑1 (MQ=255)                                                                                                                                   aCGCCGCGCGCCTTCGAGGGCGACCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGGCGccc             >  8:20609/1‑147 (MQ=255)                                                                                                                                       cgcgcGCCTTCGAGGGCGACCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTc                          >  3:59802/5‑131 (MQ=255)                                                                                                                                       cgcgcGCCTTCGAGGGCGACCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTc                          <  4:59802/127‑1 (MQ=255)                                                                                                                                         gTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAgcg      >  2:291707/1‑149 (MQ=255)                                                                                                                                             cccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATg  <  6:33200/149‑1 (MQ=255)                                                                                                                                             cccTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATg  >  6:305975/1‑149 (MQ=255)                                                                                                                                               cTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGc                                              <  1:228744/103‑1 (MQ=255)                                                                                                                                               cTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGc                                              >  2:228744/1‑103 (MQ=255)                                                                                                                                                  gAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGa                        >  6:15520/1‑122 (MQ=255)                                                                                                                                                  gAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGa                        <  5:15520/122‑1 (MQ=255)                                                                                                                                                  gAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAg                    >  8:470/1‑126 (MQ=255)                                                                                                                                                  gAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAg                    <  7:470/126‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              GCTCCACGCACAGGTTCTGCCAGTCCAGCACTTCGGCATGCTCCAGTACCAGCAGCACGAACACGCGCTGGATCGGCAGCAGCTGGTAGTCCCAGCCCTTCTGCAGGCCCTGCATGGCGACCACCTGGGCACGCCGGTCGCCCTCGAAGGCGCGCGGCGTGTCGCGGTAGATCATGCGTGGCAGCTGGTCGAGCAAAATCAGCAGGCCCAGCCAGCCCTGCGGGGTTTGCTGCCACTCTTCGAGCCCACCCGCCAGGGCGTGCTCGACCAGGTCGCCAAACAGCGCATG  >  NC_002947/5741748‑5742036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑