Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,777,227 C→G 7.7% T515S (ACC→AGC)  betA‑II → choline dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,777,2270CG 7.7% 159.4 / 4.4 52T515S (ACC→AGC) betA‑IIcholine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (25/22);  new base G (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

CGAAACCGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAA  >  NC_002947/5777093‑5777372
                                                                                                                                      |                                                                                                                                                 
cGAAACCGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAAc                                                                                                                                            <  8:102428/142‑1 (MQ=255)
cGAAACCGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAAc                                                                                                                                            >  7:102428/1‑142 (MQ=255)
      cGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGccacca                                                                                                                               >  7:27545/1‑149 (MQ=255)
      cGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGcc                                                                                                                                   >  8:165413/1‑145 (MQ=255)
      cGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGcc                                                                                                                                   <  7:165413/145‑1 (MQ=255)
       gCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCcaccac                                                                                                                              <  8:27545/149‑1 (MQ=255)
              aCCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCAt                                                                                                                        >  6:27748/1‑148 (MQ=255)
              aCCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTACACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCAt                                                                                                                        >  1:277863/1‑148 (MQ=255)
                 cGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATc                                                                                                                    <  5:27748/149‑1 (MQ=255)
                           gTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCgag                                                                                                              >  8:2691/1‑145 (MQ=255)
                               aaGATGGGCACCGACGACATGGCAGTGGTAGATGGTGAATGCCGCGTGCACGGCATTAAGGGCCTGCGCGTGGTCGATTCGTCGATCATGCCGGTGATCATCACCTTATACCTAAACGCCACCACGATCATGATCGCCGAGAAGATTTc                                                                                                      <  5:334944/149‑1 (MQ=255)
                                aGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGcc                                                                                                                 <  6:356730/137‑1 (MQ=255)
                                aGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGcc                                                                                                                 >  5:356730/1‑137 (MQ=255)
                                 gATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGAGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCg                                                                                                     >  1:74440/1‑148 (MQ=255)
                                                             gATGGTTAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATc                                                                                                                          >  6:214394/1‑99 (MQ=255)
                                                             gATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGcc                                                                        >  7:210140/1‑149 (MQ=255)
                                                                   gAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCgcag                                                                  <  7:187233/149‑1 (MQ=255)
                                                                   gAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCgcag                                                                  >  8:130147/1‑149 (MQ=255)
                                                                     aGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCg                                                                                                                <  4:290725/101‑1 (MQ=255)
                                                                     aGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCg                                                                                                                >  3:290725/1‑101 (MQ=255)
                                                                      ggCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCAc                                                               <  2:141877/149‑1 (MQ=255)
                                                                         cgcgTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGc                                                            <  7:130147/149‑1 (MQ=255)
                                                                                     aTGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGc                                                                                   >  4:110385/1‑114 (MQ=255)
                                                                                          gggCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCgg                                           <  8:210140/149‑1 (MQ=255)
                                                                                          gggCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGAGCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCACGTAGTACTTGGCgg                                           <  2:277863/149‑1 (MQ=255)
                                                                                                   cgTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCAc                                  <  5:226565/149‑1 (MQ=255)
                                                                                                        tCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAGGTACTACGAGGCGGGGGATGCACCAGt                              >  5:43999/1‑148 (MQ=255)
                                                                                                           aTGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGc                                                                                   >  8:183370/1‑92 (MQ=255)
                                                                                                           aTGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGc                                                                                   <  7:183370/92‑1 (MQ=255)
                                                                                                              ccTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGt                              >  5:319537/1‑142 (MQ=255)
                                                                                                              ccTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGt                              <  6:319537/142‑1 (MQ=255)
                                                                                                               cTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCa                                >  8:199877/1‑139 (MQ=255)
                                                                                                               cTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCa                                <  7:199877/139‑1 (MQ=255)
                                                                                                               cTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGc                       >  3:222985/1‑148 (MQ=255)
                                                                                                               cTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGACGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGc                       >  1:204558/1‑148 (MQ=255)
                                                                                                                tCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGt                              >  5:230239/1‑140 (MQ=255)
                                                                                                                tCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGt                              <  6:230239/140‑1 (MQ=255)
                                                                                                                  gATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAGGTACTACGCGGCGGGGGATGCACCAGTGAAAGGCaa                     <  6:43999/147‑1 (MQ=255)
                                                                                                                   aTCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGcc                  >  3:257394/1‑149 (MQ=255)
                                                                                                                   aTCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGcc                  <  4:222985/149‑1 (MQ=255)
                                                                                                                     gttGCCGGTGATGATCAGCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGa                >  3:320200/3‑149 (MQ=255)
                                                                                                                      ttGCCGGTGATGATCAGCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCAcc                                                              <  3:345212/101‑1 (MQ=255)
                                                                                                                      ttGCCGGTGATGATCAGCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCAcc                                                              >  4:345212/2‑102 (MQ=255)
                                                                                                                               atgatcaGCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCgtgaagt      <  4:320200/146‑1 (MQ=255)
                                                                                                                               atcatcACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCAc                                  <  8:111761/121‑1 (MQ=255)
                                                                                                                               atcatcACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCgtgaa        <  2:74440/147‑1 (MQ=255)
                                                                                                                                tcatcaCCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCgtgaagtg     <  4:257394/149‑1 (MQ=255)
                                                                                                                                tcatcaCCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCgtgaagt      >  6:305735/1‑148 (MQ=255)
                                                                                                                                   tcaCCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCAc                                  >  4:299321/1‑117 (MQ=255)
                                                                                                                                   tcaCCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGaaa  >  1:1091/1‑149 (MQ=255)
                                                                                                                                   tcaCCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGaaa  >  7:223438/1‑149 (MQ=255)
                                                                                                                                   tcaACGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGAGTGAAGTGaaa  >  7:223442/1‑149 (MQ=255)
                                                                                                                                      |                                                                                                                                                 
CGAAACCGCCTTCCACCCGTCCTGCTCGTGCAAGATGGGCACCGACGACATGGCAGTGGTCGATGGTGAAGGCCGCGTGCACGGCATGAAGGGCCTGCGCGTGGTCGATGCCTCGATCATGCCGCTGATCATCACCGGCAACCTCAACGCCACCACGATCATGATCGCCGAGAAGATTTCGGACAAGATCCGTGGTCGCAAGCCACTGCCGCGCAGCACCGCCAAGTACTACGTGGCGGGGGATGCACCAGTGAAAGGCAAGCCGATGCGTGAAGTGAAA  >  NC_002947/5777093‑5777372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑