Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 545,981 A→C 7.4% intergenic (+21/‑52) rpoC → / → PP_mr08 DNA‑directed RNA polymerase subunit beta'/rpsL_psuedo ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947545,9810AC 7.4% 165.1 / 4.7 54intergenic (+21/‑52)rpoC/PP_mr08DNA‑directed RNA polymerase subunit beta'/rpsL_psuedo ncRNA
Reads supporting (aligned to +/- strand):  ref base A (28/22);  new base C (2/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

GTCTGGCCTACCACAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCT  >  NC_002947/545845‑546129
                                                                                                                                        |                                                                                                                                                    
gTCTGGCCTACCACAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGc                                                                                                                                            <  1:98369/147‑1 (MQ=255)
gTCTGGCCTACCACAGCGACCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGc                                                                                                                                            <  7:134205/147‑1 (MQ=255)
   tGGCCTACCACAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGatc                                                                                                                                        >  4:15457/1‑148 (MQ=255)
      ccTACCACAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCg                                                                                                                                    >  5:173598/1‑149 (MQ=255)
       cTACCACAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCgg                                                                                                                                   >  7:40913/1‑149 (MQ=255)
             caGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGCAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACAt                                                                                                                             >  7:302053/1‑149 (MQ=255)
             caGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACAt                                                                                                                             <  3:15457/149‑1 (MQ=255)
               gcgagcgCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATg                                                                                                                            >  7:199751/1‑148 (MQ=255)
                cgagcgCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCgg                                                                                                                                   <  4:74065/140‑1 (MQ=255)
                cgagcgCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCgg                                                                                                                                   >  3:74065/1‑140 (MQ=255)
                     gcAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGtt                                                                                                                     >  4:63000/1‑149 (MQ=255)
                     gcAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGtt                                                                                                                     <  3:63000/149‑1 (MQ=255)
                          cGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCga                                                                                                                <  8:40913/149‑1 (MQ=255)
                              gCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCgatgat                                                                                                            >  8:110174/1‑149 (MQ=255)
                               ccGTGATGCCGACAAACCGCTGCGAGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATc                                                                                                           <  6:358010/149‑1 (MQ=255)
                                 gTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTCATGTCGCCGATGATcgc                                                                                                         <  2:56231/149‑2 (MQ=25)
                                         gACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCAACATTTCATGTCGCCga                                                                                                                >  2:337291/1‑134 (MQ=255)
                                         gACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCAACATTTCATGTCGCCga                                                                                                                <  1:337291/134‑1 (MQ=255)
                                          aCAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAgg                                                                                                <  8:199751/149‑1 (MQ=255)
                                            aaaCCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGc                                                                                              >  8:177341/1‑149 (MQ=255)
                                                 gCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTCATGTCGCCgat                                                                                                               <  3:214706/127‑1 (MQ=21)
                                                 gCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTCATGTCGCCgat                                                                                                               >  4:214706/1‑127 (MQ=21)
                                                       tgtAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGt                                                                                   >  4:302427/1‑149 (MQ=255)
                                                        gtAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCggg                                                                                          >  6:183300/1‑141 (MQ=255)
                                                        gtAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCggg                                                                                          <  5:183300/141‑1 (MQ=255)
                                                           aGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCtt                                                                                <  7:110174/148‑1 (MQ=255)
                                                                cAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCg                                                                                            >  3:3655/1‑131 (MQ=255)
                                                                       gTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTc                                                                                  >  4:226647/1‑134 (MQ=255)
                                                                       gTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTc                                                                                  <  3:226647/134‑1 (MQ=255)
                                                                        tGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGc                                                                      >  4:258052/1‑145 (MQ=255)
                                                                        tGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGa                                                                  <  7:177341/149‑1 (MQ=255)
                                                                               tGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt                                                           >  2:106101/1‑149 (MQ=255)
                                                                                gCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt                                                           >  3:25222/1‑148 (MQ=255)
                                                                                 cACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTa                                                         <  1:106101/149‑1 (MQ=255)
                                                                                  aCTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt                                                           >  1:213565/1‑146 (MQ=255)
                                                                                              gcTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTAcc                                             >  5:297114/1‑148 (MQ=255)
                                                                                              gcTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTAcc                                             <  6:297114/148‑1 (MQ=255)
                                                                                                 gAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCttt                                                          <  7:10855/132‑1 (MQ=255)
                                                                                                 gAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCttt                                                          >  8:10855/1‑132 (MQ=255)
                                                                                                      ccAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAAttt                                    >  4:67772/1‑149 (MQ=255)
                                                                                                       cAGCGGTAATTACGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTg                                   <  8:302053/149‑1 (MQ=255)
                                                                                                       cAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTg                                   >  1:319407/1‑149 (MQ=255)
                                                                                                         gCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGt                                 <  3:67772/149‑1 (MQ=255)
                                                                                                         gCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGt                                 >  8:202025/1‑149 (MQ=255)
                                                                                                            gTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt                                                           >  7:329949/1‑120 (MQ=255)
                                                                                                            gTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCtt                                                           <  8:329949/120‑1 (MQ=255)
                                                                                                             tAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAgg                             <  6:173598/149‑1 (MQ=255)
                                                                                                                ttAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATACTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTc                          >  4:89992/1‑149 (MQ=255)
                                                                                                                     tACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGtct                     >  2:307686/1‑149 (MQ=255)
                                                                                                                          gacgaGGCCCCGGCCCTCCCCGCCCGATCATCGGCAACATTTAATGTCGCCGATGATCGCCCGAGGCTTGACGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGcc                 >  4:322464/5‑148 (MQ=14)
                                                                                                                                gCCGCGGCAAGCCTCGGGCTATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAAttttt          <  7:202025/149‑1 (MQ=255)
                                                                                                                                 ccccGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCaa               >  1:7566/1‑143 (MQ=255)
                                                                                                                                 ccccGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCaa               <  2:7566/143‑1 (MQ=255)
                                                                                                                                   ccGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGc       >  4:162227/1‑149 (MQ=255)
                                                                                                                                        aaGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCt  >  1:57313/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                                    
GTCTGGCCTACCACAGCGAGCGCAAGCGTCGCCGTGATGCCGACAAACCGCTGCGTGTAAGCGCCAGTGAAGTGGAAGCTGCACTGACCGAAGCGCTGAATTCCAGCGGTAATTAAGTACAGGGCAAGGCCCCGGCAAGCCTCGGGCGATCATCGGCGACATGAAATGTTGCCGATGATCGGGCGGGGAGGGCCGGGGCCTCGTCTTGACTGGGTGCAAGATCCTCTTTAGACTTTTGTACCCTTAAATTTGGTGAGGCTCCGTCTCGCCAATTTTTGGCTTTCT  >  NC_002947/545845‑546129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑