Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,821,897 T→A 7.2% L173Q (CTG→CAG)  metW → methionine biosynthesis protein MetW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,821,8970TA 7.2% 199.3 / 3.9 68L173Q (CTG→CAG) metWmethionine biosynthesis protein MetW
Reads supporting (aligned to +/- strand):  ref base T (31/32);  new base A (3/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCACCA  >  NC_002947/5821749‑5822041
                                                                                                                                                    |                                                                                                                                                
tGCCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCt                                                                                                                                                  >  3:145791/1‑149 (MQ=255)
   cGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGc                                                                                                                                               <  4:145791/149‑1 (MQ=255)
     cTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCgg                                                                                                                                             <  7:34920/149‑1 (MQ=255)
           ccTCGCGACCAAAGGCCGCATGCCGGTATCGGACGTCATGCCGTAAACCCGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGAcc                                                                                                                                       <  2:51524/149‑1 (MQ=255)
            cTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCa                                                                                                                                      >  4:242247/1‑149 (MQ=255)
               gCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCTAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTg                                                                                                                                                 <  5:41781/135‑1 (MQ=255)
               gCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTg                                                                                                                                                 >  6:41781/1‑135 (MQ=255)
               gCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACtt                                                                                                                                   <  6:164223/149‑1 (MQ=255)
                    cAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAcc                                                                                                                              >  4:124699/1‑149 (MQ=255)
                     aaaGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCg                                                                                                                             >  6:147495/1‑149 (MQ=255)
                           cGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACCCGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgg                                                                                                                       <  3:182043/149‑1 (MQ=255)
                              aTGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggtg                                                                                                                    >  4:8695/1‑149 (MQ=255)
                                   ggTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggtggggtg                                                                                                               <  8:315666/149‑1 (MQ=255)
                                         ggACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGc                                                                                                         >  1:217520/1‑149 (MQ=255)
                                                aTGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGc                                                                                                   <  7:129906/148‑1 (MQ=255)
                                                   ccGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACATGTGCACGAACGCCCGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACATGCACCGCAACGCGTGCGGTGCCCGGCTAAGGCCTaa                                                                                               >  6:354317/1‑149 (MQ=255)
                                                        taCCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtctt                                                                                           <  5:147495/148‑1 (MQ=255)
                                                           cTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAg                                                                                       <  2:217520/149‑1 (MQ=255)
                                                             ggTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttct                                                                                         >  3:158967/1‑145 (MQ=255)
                                                             ggTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttct                                                                                         <  4:158967/145‑1 (MQ=255)
                                                                acaaccCGCCTAAAATCCACTTCTGAACATTCGCCGATTTCTAAGAACTTGTGCACTAACGCCGGGACAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGc                                                                                                   <  4:337231/132‑1 (MQ=255)
                                                                acaacaCGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGc                                                                                                   >  3:337231/1‑132 (MQ=255)
                                                                     acGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCgg                                                                             <  3:242247/149‑1 (MQ=255)
                                                                     acGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCgg                                                                             >  5:290614/1‑149 (MQ=255)
                                                                       gCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCa                                                                           >  6:351318/1‑149 (MQ=255)
                                                                        ccGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTGGTGCACGATCGCCGGGCGATGGTACTCGACGGCCTGGGGTTCGTCCACTTGGACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCAt                                                                          <  5:354317/149‑1 (MQ=255)
                                                                           aaCATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCTCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTa                                                                                        <  7:94409/132‑1 (MQ=255)
                                                                           aaCATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTa                                                                                        >  8:94409/1‑132 (MQ=255)
                                                                              tctcACTGCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTAc                                                                      <  5:135615/144‑1 (MQ=255)
                                                                              aTCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttct                                                                                         <  8:335463/128‑1 (MQ=255)
                                                                              aTCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttct                                                                                         >  7:335463/1‑128 (MQ=255)
                                                                                  aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTgg                                                                                                                                                <  2:119761/69‑1 (MQ=255)
                                                                                  aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACAGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTAc                                                                      >  6:135615/1‑143 (MQ=255)
                                                                                        gCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGt                                                          >  4:129105/1‑149 (MQ=255)
                                                                                         cACCTTCGCCGACTTCGAAGAACTTGTGCACGATCGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGt                                                                 >  8:99325/1‑141 (MQ=255)
                                                                                         cACCTTCGCCGACTTCGAAGAACTTGTGCACGATCGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGt                                                                 <  7:99325/141‑1 (MQ=255)
                                                                                         cACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCTCCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTg                                                                                    >  1:111059/1‑122 (MQ=255)
                                                                                         cACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTg                                                                                    <  2:111059/122‑1 (MQ=255)
                                                                                         cACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTcagca                                                            <  5:351318/146‑1 (MQ=255)
                                                                                         cACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTc                                                         >  1:143361/1‑149 (MQ=255)
                                                                                            cTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggt                                                                                                                     >  6:353448/1‑86 (MQ=255)
                                                                                            cTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCt                                                                        <  5:126617/131‑1 (MQ=255)
                                                                                            cTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCt                                                                        >  6:126617/1‑131 (MQ=255)
                                                                                               cGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCggg                                                     <  2:143361/147‑1 (MQ=255)
                                                                                                 ccGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCCACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTg                                                 >  1:202337/1‑149 (MQ=255)
                                                                                                  cGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATc                                                                               >  8:173508/1‑118 (MQ=255)
                                                                                                  cGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATc                                                                               <  7:173508/118‑1 (MQ=255)
                                                                                                      ttCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTa                                                                                        >  2:169809/1‑105 (MQ=255)
                                                                                                              aCTTGTGCACTAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTATGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGc                                    <  3:124699/149‑1 (MQ=255)
                                                                                                                  gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggtgg                                                                                                                   <  3:329519/66‑1 (MQ=255)
                                                                                                                  gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggtgg                                                                                                                   >  4:329519/1‑66 (MQ=255)
                                                                                                                  gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcgc                                >  7:254923/1‑149 (MQ=255)
                                                                                                                      aCGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTc                            >  1:220556/1‑149 (MQ=255)
                                                                                                                      aCGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTc                            <  3:129105/149‑1 (MQ=255)
                                                                                                                                  ccAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCg                >  7:208915/1‑149 (MQ=255)
                                                                                                                                      agtggtCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGTAAGAGCTTCAGCTCGCGGTCTGACGCCCACCggag             >  4:250410/6‑148 (MQ=255)
                                                                                                                                        tACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGaa          <  3:8695/149‑1 (MQ=255)
                                                                                                                                           tCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCgg                                                      <  1:280258/102‑1 (MQ=255)
                                                                                                                                           tCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCgg                                                      >  2:280258/1‑102 (MQ=255)
                                                                                                                                             gACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGAc                        >  5:168669/1‑130 (MQ=255)
                                                                                                                                             gACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGAc                        <  6:168669/130‑1 (MQ=255)
                                                                                                                                             gACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCgga              <  4:33463/140‑1 (MQ=255)
                                                                                                                                             gACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCgga              >  3:33463/1‑140 (MQ=255)
                                                                                                                                              cccGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAGCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGCCGCCCACCGGAGGACTCGAAc    >  3:190227/2‑149 (MQ=255)
                                                                                                                                              aCCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCa                                      >  3:149951/1‑115 (MQ=255)
                                                                                                                                              aCCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCa                                      <  4:149951/115‑1 (MQ=255)
                                                                                                                                              aCCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCAc    <  6:290614/149‑1 (MQ=255)
                                                                                                                                               ccGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCAcc   <  2:220556/149‑1 (MQ=255)
                                                                                                                                                cGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCACCa  >  6:157411/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGCCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCACCA  >  NC_002947/5821749‑5822041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑