| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,825,478 | G→T | 7.0% | intergenic (‑6/+4) | trmB ← / ← thiG | tRNA (guanosine(46)‑N7)‑methyltransferase TrmB/thiazole synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,825,478 | 0 | G | T | 7.0% | 171.2 / 3.8 | 57 | intergenic (‑6/+4) | trmB/thiG | tRNA (guanosine(46)‑N7)‑methyltransferase TrmB/thiazole synthase |
| Reads supporting (aligned to +/- strand): ref base G (26/27); new base T (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01 | |||||||||||
ATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCTGGGCGTGG > NC_002947/5825333‑5825620 | aTGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGctc < 1:117460/149‑1 (MQ=255)aTGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGctc < 4:157474/149‑1 (MQ=255) gccgccTTGGTCCAGACCGCTTTGCTGGCCTTCGGTCATGCGCCGGGCTCGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCa < 1:106410/149‑1 (MQ=255) gccgccTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGCTGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGAt > 3:88749/1‑147 (MQ=255) gccgccTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCa < 3:18988/149‑1 (MQ=255) gccTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGAc > 7:18879/1‑149 (MQ=255) cTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGAcc > 8:206197/1‑148 (MQ=255) cTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCa > 6:146329/1‑149 (MQ=255) cAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAg‑c < 6:71777/148‑1 (MQ=255) aGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAg‑cgg < 6:199280/149‑1 (MQ=255) gACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAg‑cggc < 7:206197/149‑1 (MQ=255) ccGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCga < 4:197546/149‑1 (MQ=255) cgcgTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTa < 6:70329/124‑1 (MQ=255) cgcgTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTa > 5:70329/1‑124 (MQ=255) cgTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCgagga < 4:88749/149‑1 (MQ=255) gTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCgaggag < 5:161862/149‑1 (MQ=255) gCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTg < 3:346192/148‑1 (MQ=255) ggTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATaga < 5:316633/149‑1 (MQ=255) cATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAg > 3:153767/1‑114 (MQ=255) cATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAg < 4:153767/114‑1 (MQ=255) tGCGCCAGGCACGCATCACGAAGCTCTTGATGAGGCGGTGTGGGCGGGCTTCGCCGTAGGGGCTGATCGGCGTATCGTTCGATTCAGTCATCACTAGCTCTTACTTGATCAGACCATCCAC‑CGGCGAGGAGGCGATGGCATAGAGttt > 1:33659/1‑148 (MQ=255) gccGCATCAAGAGCTTCGTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATa > 6:126628/3‑149 (MQ=255) aTCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGAc > 5:204016/1‑148 (MQ=255) gAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATagag > 4:162740/1‑126 (MQ=255) gAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATagag < 3:162740/126‑1 (MQ=255) tctTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAg > 6:61583/1‑149 (MQ=255) gATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGtt < 2:60321/119‑1 (MQ=255) aTGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAGCCGGCGAGGAGGCGCTGGCATAGAGTttcttc < 1:207872/124‑1 (MQ=255) aTGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTttcttc > 2:207872/1‑123 (MQ=255) ggcggTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACGAGCCAGGTAGGCCATGCGa > 8:321356/1‑149 (MQ=255) ggctggctTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTTCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCAt < 7:200771/136‑1 (MQ=255) ggcgggcTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTTCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCAt < 7:195424/136‑1 (MQ=255) ggcgggcTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTTCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCAt > 8:195424/1‑136 (MQ=255) ggcgggcTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTTCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCAt > 8:200771/1‑136 (MQ=255) ggcgggcTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGAc > 4:47315/1‑148 (MQ=255) ttCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCgt > 3:337562/1‑149 (MQ=255) gCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCgtgt < 5:146329/148‑1 (MQ=255) ccGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGttt > 6:165573/1‑149 (MQ=255) gggggTGATCGGCGTATCGTGCGATTCATTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATg < 8:18879/148‑1 (MQ=255) gggggTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATg < 5:61583/148‑1 (MQ=255) gCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAAt < 8:19551/124‑1 (MQ=255) gCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAAt > 7:19551/1‑124 (MQ=255) gCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATc < 3:47315/149‑1 (MQ=255) tCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCAc > 3:133823/1‑149 (MQ=255) gCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCgg > 5:105453/1‑135 (MQ=255) gCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCgg < 6:105453/135‑1 (MQ=255) ttCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGgctgct > 3:25118/1‑149 (MQ=255) cAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtgg > 7:99491/1‑149 (MQ=255) aGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtggg > 2:41476/1‑149 (MQ=255) aGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtgg < 4:133823/148‑1 (MQ=255) atcaGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATgg < 6:283143/118‑1 (MQ=255) atcaGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtgggcgtg > 7:239611/1‑149 (MQ=255) tcaGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCa < 6:209773/114‑1 (MQ=255) tcaGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCa > 5:209773/1‑114 (MQ=255) tcaGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtgggcgtgg > 5:139703/1‑149 (MQ=255) gTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtgggcgtg > 6:101842/1‑145 (MQ=255) gTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCtgggcgtg < 5:101842/145‑1 (MQ=255) | ATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATGCGCCCGGCACGCATCACGAAGCTCTTGATGCGGCGGTGTGGGCGGGCTTCGCCGTCGGGGGTGATCGGCGTATCGTGCGATTCAGTCATCAGTTGCTCTTACTTGATCAGACCATCCAG‑CGGCGAGGAGGCGCTGGCATAGAGTTTCTTCGGCATACGACCAGCCAGGTAGGCCATGCGACCCGCGACAATGGCGTGTTTCATGGCCTCGGCCATCAGCACTGGCTGCTGGGCGTGG > NC_002947/5825333‑5825620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |