Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,831,840 A→C 12.6% L102V (TTG→GTG)  ftsY ← signal recognition particle protein translocation receptor GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,831,8400AC12.6% 122.0 / 3.4 45L102V (TTG→GTG) ftsYsignal recognition particle protein translocation receptor GTPase
Reads supporting (aligned to +/- strand):  ref base A (22/17);  new base C (2/4);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCACCAGGTTGCTGACCGGCGCTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGAT  >  NC_002947/5831696‑5831982
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gcagcaCCAGGTTGCTGACCGGCGCTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGc                                                                                                                                            >  1:60292/1‑149 (MQ=255)
   gcaCCAGGTTGCTGACCGGCGCTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGc                                                                                                                                         <  8:50012/149‑1 (MQ=255)
       cAGGTTGCTGACCGGCGCTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACg                                                                                                                                     >  7:292444/1‑149 (MQ=255)
               tGACCGGCGCTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGt                                                                                                                             >  4:173889/1‑149 (MQ=255)
                       gcTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGAc                                                                                                                     >  4:34702/1‑149 (MQ=255)
                              ccTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGcc                                                                                                              <  3:34702/149‑1 (MQ=255)
                                  gACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTc                                                                                                            <  3:217596/147‑1 (MQ=255)
                                  gACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGa                                                                                                          <  2:60292/149‑1 (MQ=255)
                                      ggggCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTAc                                                                                                      <  8:292444/149‑1 (MQ=255)
                                           aGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACt                                                                                                        <  8:574/142‑1 (MQ=255)
                                           aGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACt                                                                                                        >  7:574/1‑142 (MQ=255)
                                             aCTCCACAGGGGCCGCCTGCAAGTGCATGGACGCAACCGGCTGAGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGa                                                                                                          <  6:306464/138‑1 (MQ=255)
                                             aCTCCACAGGGGAAGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGAGACCGGCTCTGGC‑TCAGGCGCCTGAAACGGCTGCGACGCAACCGGATCAGGGACGGGGGCCTCGa                                                                                                          >  5:306464/1‑138 (MQ=255)
                                                 cacaGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACgg                                                                                                                   <  4:107848/125‑1 (MQ=255)
                                                 cacaGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACgg                                                                                                                   >  3:107848/1‑125 (MQ=255)
                                                 cacaGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCggg                                                                                           >  5:68068/1‑149 (MQ=255)
                                                            ccTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCgg                                                                                                   >  1:33664/1‑130 (MQ=255)
                                                            ccTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCgg                                                                                                   <  2:33664/130‑1 (MQ=255)
                                                               gCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGAcc                                                                             >  1:219396/1‑149 (MQ=255)
                                                               gCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCCGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGAcc                                                                             >  3:181641/1‑149 (MQ=255)
                                                                cAAGGGCAGGGACGCAACCGGGGGGGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCACCCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGAc                                                                              <  4:148333/147‑1 (MQ=255)
                                                                   gggCATGGACGCAACGGGGTGCGGGGCAGGTGC‑CTCCTGCGGGTGGGATGCGCCCGGCTCTGGC‑TCCGGCGCCTGCAACGGCTGCTACGCAACCGGTTCAGGGCCGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCt                                                                         <  6:68068/149‑1 (MQ=255)
                                                                       aTGGACGCAACCGGCTGCGGGGCAGGTGCTCACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACgggg                                                                                                                 >  1:201991/1‑106 (MQ=255)
                                                                       aTGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACgggg                                                                                                                 <  2:201991/105‑1 (MQ=255)
                                                                       aTGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACggg                                                                                                                  >  8:200047/1‑104 (MQ=255)
                                                                       aTGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACggg                                                                                                                  <  7:200047/104‑1 (MQ=255)
                                                                       aTGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCgg                                                                     >  1:99245/1‑149 (MQ=255)
                                                                               aaCCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTcagc                                                               >  7:295283/1‑147 (MQ=255)
                                                                                    gCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCCGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCtgt                                                        <  4:181641/149‑1 (MQ=255)
                                                                                                 tGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACgg                                           >  5:257256/1‑149 (MQ=255)
                                                                                                   c‑caccaGCGGCTGGGATGCGACCGGCTCTGGCGTCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGc                                          >  1:42629/1‑149 (MQ=255)
                                                                                                      accaGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCt                                        >  1:173440/1‑148 (MQ=255)
                                                                                                        caGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGc                                                                                                                                            >  2:61385/1‑46 (MQ=255)
                                                                                                           cGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACgg                                  <  3:173889/149‑1 (MQ=255)
                                                                                                             gCTGGGATGCGCCGGGCTCTGGC‑GGTGGCGCGTGCACCGGCTGCGCCGCACCGGGTTCTGGGCCGGGGGCCTCGACTCCCGGCGGCGGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGt                                <  2:173440/149‑1 (MQ=255)
                                                                                                                    tGCGACGGGCTCTGGC‑TCCGGCGCCTGCACCGGCTGCGACGCAAGGGGGTCAGGGACGGGGGCCTCGACTAGCGGCGGCGGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCgggg                          <  6:257256/148‑1 (MQ=255)
                                                                                                                    tGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCggggg                         >  3:261646/1‑149 (MQ=255)
                                                                                                                         ccGGCTCTGGC‑TCAGGCGCCGGCAACCGCTGCGACCCAACCGGTTCAGGGACGGGGGCCTCGACTCCCGGCGGCGGGGGGGGGGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCtgt                    <  8:223976/149‑1 (MQ=255)
                                                                                                                          cGGCTCTGGC‑GCAGGCGCCTGCACCGGCTGCGACGCAACCGGTTCTGGGCCGGGGGCCTCGACTACCGGTGGCGGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCtgtg                   <  4:329962/149‑1 (MQ=255)
                                                                                                                                        ggCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCC‑GGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTcggcgg       >  3:60000/1‑147 (MQ=255)
                                                                                                                                        cgtcccTGAACCGGTTGCGTCGCAGCCGTTGCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTcggcgg       <  4:325703/144‑1 (MQ=38)
                                                                                                                                        cgtcccTGAACCGGTTGCGTCGCAGCCGTTGCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTcggcgg       >  3:325703/5‑148 (MQ=38)
                                                                                                                                          tcccTGAACCGGTTGCGTCGCAGCCGTTGCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTcggcgg       >  2:78667/3‑146 (MQ=255)
                                                                                                                                           gcCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTcggcgg       >  5:223532/1‑145 (MQ=255)
                                                                                                                                            ccTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGAt  <  2:42629/149‑1 (MQ=255)
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GCAGCACCAGGTTGCTGACCGGCGCTGCGGCCTCGACAGGGGCAGACTCCACAGGGGCCGCCTGCAAGGGCATGGACGCAACCGGCTGCGGGGCAGGTGC‑CACCAGCGGCTGGGATGCGACCGGCTCTGGC‑TCAGGCGCCTGCAACGGCTGCGACGCAACCGGTTCAGGGACGGGGGCCTCGACTACCGGAGGCCGGGGGGGTGCGGCGACCGGCTGCGGCTCAGCCAGCTGTGGGGCCGCAACGGCCTGAACGGGTTCGGGGGCCTGTGGCGCTTCGGCGGCAGAT  >  NC_002947/5831696‑5831982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑