Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,832,871	A→T	10.3%	K166M (AAG→ATG) ‡	PP_5112 →	peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,832,871	0	A	T	10.3%	180.4 / 7.3	65	K166M (AAG→ATG) ‡	PP_5112	peptidase
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base T (4/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCCCGCGACCGCCTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGCCCCCAACAACGCCACCCT  >  NC_002947/5832726‑5833018                                                                                                                                                   |                                                                                                                                                    cTGGCCCGCGACCGCCTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAgg                                                                                                                                                   >  7:420/1‑148 (MQ=255) cTGGCCCGCGACCGCCTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGATCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAgg                                                                                                                                                   <  8:226140/148‑1 (MQ=255)       cgcgACCGCCTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGa                                                                                                                                            <  7:112261/149‑1 (MQ=255)              gCCTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGTCCATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGtt                                                                                                                                      <  1:104379/149‑1 (MQ=255)                cTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCcg                                                                                                                                   >  5:317601/1‑149 (MQ=255)                   ccGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCcgc                                                                                                                                  <  7:184252/147‑1 (MQ=255)                     ggTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCa                                                                                                                              >  8:247887/1‑149 (MQ=255)                      gTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCAt                                                                                                                             <  4:288340/149‑1 (MQ=255)                       tAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATg                                                                                                                            <  3:153276/149‑1 (MQ=255)                             tGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTAt                                                                                                                      >  5:52021/1‑149 (MQ=255)                                 aCTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCt                                                                                                                                         >  2:222438/1‑126 (MQ=255)                                 aCTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCAACGACGACCAGCCCAATGCCAAGGCATTCGAGCt                                                                                                                                         <  1:222438/126‑1 (MQ=255)                                    ggAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCTTGGCAttgggc                                                                                                                                           <  1:90732/121‑5 (MQ=255)                                    ggAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCTTGGCAttgggc                                                                                                                                           >  2:90732/1‑117 (MQ=255)                                     gAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCa                                                                                                               <  8:420/148‑1 (MQ=255)                                           gACCGCCTTGCCAGCCTGCGCCTACCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCgg                                                                                                           >  4:183480/1‑146 (MQ=255)                                           gACCGCCTTGCCAGCCTGCGCCTACCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCgg                                                                                                           <  3:183480/146‑1 (MQ=255)                                                    gCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGcc                                                                                                                         <  2:214392/123‑1 (MQ=255)                                                    gCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGcc                                                                                                                         >  1:214392/1‑123 (MQ=255)                                                       aGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGAc                                                                                            >  6:262271/1‑149 (MQ=255)                                                                ctgcctgcCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTg                                                                                   <  5:262271/149‑1 (MQ=255)                                                                ctgcctgcCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTg                                                                                   >  2:220112/1‑149 (MQ=255)                                                                       ccGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGcc                                                                            <  7:247887/149‑1 (MQ=255)                                                                              gTTCATCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACAGACGAACAGCCCAATGCCAAGCCATTCGAGCTGTTCCGCGCCATGGCCTAACCGGCCAGCGGCTACCAAACGCCTAcc                                                                                           <  7:223027/127‑1 (MQ=255)                                                                              gTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTg                                                                      >  6:272004/1‑148 (MQ=255)                                                                               ttCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATc                                                                                                                     <  6:161745/100‑1 (MQ=255)                                                                               ttCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATc                                                                                                                     >  5:161745/1‑100 (MQ=255)                                                                                     cgcgAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGAt                                                                                >  6:41368/1‑131 (MQ=255)                                                                                     cgcgAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGAt                                                                                <  5:41368/131‑1 (MQ=255)                                                                                     cgcgAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCAt                                                              >  5:228252/1‑149 (MQ=255)                                                                                            tCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAgc                                                                  <  3:188957/138‑1 (MQ=255)                                                                                            tCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAgc                                                                  >  4:188957/1‑138 (MQ=255)                                                                                              gAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTTGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTc                                                       >  5:327963/1‑147 (MQ=255)                                                                                              gAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGa                                                     <  6:317601/149‑1 (MQ=255)                                                                                                    catcaAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAAc                                                <  5:272004/149‑1 (MQ=255)                                                                                                     atcaAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACt                                               >  2:72298/1‑149 (MQ=255)                                                                                                        aaGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTTGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTgc                                             <  6:327963/148‑1 (MQ=255)                                                                                                        aaGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTgc                                             <  6:228252/148‑1 (MQ=255)                                                                                                        aaGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTgc                                             <  1:158940/148‑1 (MQ=255)                                                                                                         aGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTgcgc                                           >  8:63709/1‑149 (MQ=255)                                                                                                         aGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTgcgc                                           <  7:63709/149‑1 (MQ=255)                                                                                                              gaaCGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTg                                      >  2:297629/1‑149 (MQ=255)                                                                                                               aaCGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGAc                                                                         <  5:290378/113‑1 (MQ=255)                                                                                                               aaCGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGAc                                                                         >  6:290378/1‑113 (MQ=255)                                                                                                                aCGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAg                                                          <  1:169518/127‑1 (MQ=255)                                                                                                                aCGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAg                                                          >  2:169518/1‑127 (MQ=255)                                                                                                                  gccgccTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTAc                                  <  1:72298/149‑1 (MQ=255)                                                                                                                    cgccTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGa                                <  1:220112/149‑1 (MQ=255)                                                                                                                      ccTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGat                                                    <  6:79444/127‑2 (MQ=255)                                                                                                                      ccTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAg                                                    <  6:74370/127‑1 (MQ=255)                                                                                                                       cTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGa                                                     >  2:330932/1‑125 (MQ=255)                                                                                                                       cTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGa                                                     <  1:330932/125‑1 (MQ=255)                                                                                                                         gcgcACCGACGACCAGCCCAATGTCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCt                           >  5:223830/1‑149 (MQ=255)                                                                                                                           gcACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCAc                                        <  7:196473/134‑1 (MQ=255)                                                                                                                           gcACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCAc                                        >  8:196473/1‑134 (MQ=255)                                                                                                                            cACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGAt                                                                                >  3:103201/1‑93 (MQ=255)                                                                                                                            cACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGAt                                                                                <  4:103201/93‑1 (MQ=255)                                                                                                                                       aGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGCCCCcaa              >  1:15946/1‑148 (MQ=255)                                                                                                                                        ctcgaaTGCCTTGGCATT‑GGGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGCCCCcaacaa           >  8:57366/5‑149 (MQ=255)                                                                                                                                         tcgaaTGCCTTGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGccccc                >  2:279637/4‑144 (MQ=255)                                                                                                                                         tcgaaTGCCTTGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGccccc                <  1:256827/141‑1 (MQ=255)                                                                                                                                         tcgaaTGCCTTGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGccccc                <  1:279637/141‑1 (MQ=255)                                                                                                                                         tcgaaTGCCTTGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGccccc                >  2:256827/4‑144 (MQ=255)                                                                                                                                         cccAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAg                                                    <  1:329987/108‑1 (MQ=255)                                                                                                                                         cccAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAg                                                    >  2:329987/1‑108 (MQ=255)                                                                                                                                           caatgccaatgcATTCGAGCTGTTCCGCGCCATGGCCTATAAGTCCAGCGTCTACAACACGCAGAACATTGGCTGGATGGCCTACATGGATCGCAGTAATGTCGAGGCACTGCGCCACTGGTACTAGtaa                            >  7:131454/1‑128 (MQ=255)                                                                                                                                                  aaGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGCCCCCAACAACGCCACCCt  >  3:338251/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CTGGCCCGCGACCGCCTGCCGGTAGCCCTGGAACTGGAGGCCGACCGCCTTGCCAGCCTGCGCCTGCCTGCCGACGAGTTCAGCCGCGAAATCGAGGT‑CATCAAGGAAGAACGCCGCCTGCGCACCGACGACCAGCCCAATGCCAAGGCATTCGAGCTGTTCCGCGCCATGGCCTATCCGGCCAGCGGCTACCACACGCCGACCATTGGCTGGATGGCCGACCTGGAGCGCATGAAGGTCGAGGAACTGCGCCACTGGTACGAGTCCTGGTACGCCCCCAACAACGCCACCCT  >  NC_002947/5832726‑5833018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑