Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,882,091 C→G 7.4% Q218E (CAG→GAG) ‡ serA → bifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,882,0910CG 7.4% 167.7 / 2.6 53Q218E (CAG→GAG) ‡serAbifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACG  >  NC_002947/5881945‑5882232
                                                                                                                                                  |                                                                                                                                             
tGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAg                                                                                                                                             >  5:182949/1‑149 (MQ=255)
  gCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCGGAACGAGCTGCTGGTCCTGGCTGACATCGTCTCGCTGCACCTGCCTGAGCTGCCATCCACCCAGTg                                                                                                                                           >  3:234658/1‑149 (MQ=255)
            tGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGc                                                                                                                                 <  8:249438/149‑1 (MQ=255)
             gggCATGCGGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGc                                                                                                                                 >  3:291548/1‑148 (MQ=255)
              ggCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCga                                                                                                                               <  6:182949/149‑1 (MQ=255)
                 aTGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGaa                                                                                                                            <  6:164224/149‑1 (MQ=255)
                           aCTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGc                                                                                                                                 <  8:204980/134‑1 (MQ=255)
                           aCTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGc                                                                                                                                 >  7:204980/1‑134 (MQ=255)
                                  cGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATg                                                                                                            <  7:718/148‑1 (MQ=255)
                                   gACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATgaa                                                                                                          <  6:192091/149‑1 (MQ=255)
                                     cccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGAt                                                                                                             >  1:196669/1‑144 (MQ=255)
                                     cccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGAt                                                                                                             <  2:196669/144‑1 (MQ=255)
                                      ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgatggca                                                                                                                                    >  7:25153/1‑117 (MQ=255)
                                      ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgatggca                                                                                                                                    <  8:25153/120‑4 (MQ=255)
                                      ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGAt                                                                                                             <  5:318521/143‑1 (MQ=255)
                                      ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGAt                                                                                                             >  6:318521/1‑143 (MQ=255)
                                        gCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAgg                                                                                                     <  2:228199/149‑1 (MQ=255)
                                          tGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGgc                                                                                                   >  6:83322/1‑149 (MQ=255)
                                            aCCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGgcgc                                                                                                 <  4:291548/149‑1 (MQ=255)
                                                     ccACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGAt                                                                                        <  3:336085/149‑1 (MQ=255)
                                                      cACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGa                                                                                                                         >  2:196644/1‑115 (MQ=255)
                                                       aCTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATc                                                                                       >  7:69037/1‑148 (MQ=255)
                                                                 gCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAAt                                                                                                                      >  2:105957/1‑107 (MQ=255)
                                                                 gCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAAt                                                                                                                      <  1:105957/107‑1 (MQ=255)
                                                                         ggTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCt                                                                                           <  8:170853/126‑1 (MQ=255)
                                                                         ggTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCt                                                                                           >  7:170853/1‑126 (MQ=255)
                                                                             aCCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGt                                                                >  1:46463/1‑149 (MQ=255)
                                                                                 gCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAg                                                            <  5:160945/149‑1 (MQ=255)
                                                                                         gAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGacca                                                    >  2:89401/1‑149 (MQ=255)
                                                                                           gctgctGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGAGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGaccacc                                                  >  8:262706/1‑149 (MQ=255)
                                                                                               ctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGCCCACCTGGc                                              >  3:317339/1‑149 (MQ=255)
                                                                                               ctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCCAGCTCGaccacggt                                                <  5:83322/147‑4 (MQ=255)
                                                                                                  ggCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGcc                                             <  6:71366/147‑1 (MQ=255)
                                                                                                   gCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCt                                          >  5:259510/1‑149 (MQ=255)
                                                                                                            aCATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAg                                 >  3:55530/1‑149 (MQ=255)
                                                                                                              aTCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGa                               >  5:355218/1‑149 (MQ=255)
                                                                                                              aTCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGa                               <  1:89401/149‑1 (MQ=255)
                                                                                                                 gTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGGGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACaa                            <  3:25125/149‑1 (MQ=255)
                                                                                                                  tctcGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCACTGCCATCAAGGACAAg                           >  3:243638/1‑149 (MQ=255)
                                                                                                                       cTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCt                      >  1:90473/1‑149 (MQ=255)
                                                                                                                             gTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCAc                        >  5:234012/1‑141 (MQ=255)
                                                                                                                             gTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCAc                        <  6:234012/141‑1 (MQ=255)
                                                                                                                                ccTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCACTGCCATCAAGGACAAGCACCTGATCGgcg              <  4:243638/148‑1 (MQ=255)
                                                                                                                                 cTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGcgcc            <  2:90473/149‑1 (MQ=255)
                                                                                                                                   gAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGcgccgc          >  8:242328/1‑149 (MQ=255)
                                                                                                                                    gccgatcATCCACTGGGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATc                  <  4:292708/134‑1 (MQ=255)
                                                                                                                                    gccgatcATCCACTGGGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATc                  >  3:292708/7‑140 (MQ=255)
                                                                                                                                    aGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCgccgcc         <  8:69037/149‑1 (MQ=255)
                                                                                                                                       tGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACaa                            >  3:55114/1‑127 (MQ=255)
                                                                                                                                       tGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACaa                            <  4:55114/127‑1 (MQ=255)
                                                                                                                                       ngCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATc      >  2:116840/2‑149 (MQ=255)
                                                                                                                                         ccATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATc      <  6:355218/147‑1 (MQ=255)
                                                                                                                                           aTCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACg  >  3:92456/1‑149 (MQ=255)
                                                                                                                                                 ccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCt                                          >  7:59292/1‑103 (MQ=255)
                                                                                                                                                 ccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCt                                          <  8:59292/103‑1 (MQ=255)
                                                                                                                                                 ccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCAc                        <  2:272522/121‑1 (MQ=255)
                                                                                                                                                 ccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCAc                        >  1:272522/1‑121 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
TGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACG  >  NC_002947/5881945‑5882232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑