Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,882,348 C→T 9.8% N303N (AAC→AAT) ‡ serA → bifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,882,3480CT 9.8% 196.3 / 8.4 71N303N (AAC→AAT) ‡serAbifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base T (4/3);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGCGTG  >  NC_002947/5882208‑5882492
                                                                                                                                            |                                                                                                                                                
cACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCt                                                                                                                                          <  7:97126/149‑1 (MQ=255)
      aTCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGaa                                                                                                                                      <  7:291055/147‑1 (MQ=255)
          gcgcCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGcc                                                                                                                                <  4:289316/149‑1 (MQ=255)
              cgccATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCgag                                                                                                                             >  1:340785/1‑148 (MQ=255)
               gccATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCgaga                                                                                                                            >  1:173937/1‑148 (MQ=255)
                  aTCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACt                                                                                                                        >  6:54806/1‑149 (MQ=255)
                      aCGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTc                                                                                                                    <  5:54806/149‑1 (MQ=255)
                        gtgtTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTc                                                                                                                    >  8:18578/1‑147 (MQ=255)
                        gtgtTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCaa                                                                                                                  <  4:45939/149‑1 (MQ=255)
                                 gTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGa                                                                                                         >  4:206342/1‑149 (MQ=255)
                                       ccACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAg                                                                                                                 <  5:239253/135‑1 (MQ=255)
                                       ccACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAg                                                                                                                 >  6:239253/1‑135 (MQ=255)
                                                aaCGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGACAACATCGGCCTGGAAGTGGc                                                                                                                                 >  5:337223/1‑110 (MQ=255)
                                                aaCGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGACAACATCGGCCTGGAAGTGGc                                                                                                                                 <  6:337223/110‑1 (MQ=255)
                                                    acgaAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCg                                                                                                                                              <  7:95748/93‑1 (MQ=255)
                                                    acgaAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCg                                                                                                                                              >  8:95748/1‑93 (MQ=255)
                                                    acgaAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTcc                                                                                      >  3:167917/1‑149 (MQ=255)
                                                      gaAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCGATGTTGGCCTgg                                                                                                                                        <  4:41919/97‑1 (MQ=255)
                                                      gaAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCGATGTTGGCCTgg                                                                                                                                        >  3:41919/1‑97 (MQ=255)
                                                          agTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTc                                                                                <  7:18578/149‑1 (MQ=255)
                                                            ttCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCaa                                                                              >  3:112218/1‑149 (MQ=255)
                                                               gagaGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGt                                                                                                  >  3:229232/1‑126 (MQ=255)
                                                               gagaGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGt                                                                                                  <  4:229232/126‑1 (MQ=255)
                                                               gagaGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTa                                                                                                 >  6:41606/1‑127 (MQ=255)
                                                               gagaGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTa                                                                                                 <  5:41606/127‑1 (MQ=255)
                                                                agagCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTc                                                                          <  2:340785/149‑1 (MQ=255)
                                                                      cGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCgg                                                                      >  2:288923/1‑147 (MQ=255)
                                                                              ggCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCt                                                            <  1:288923/149‑1 (MQ=255)
                                                                                ccTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGc                                                          <  1:213651/149‑1 (MQ=255)
                                                                                 cTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGCCAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGc                                                          >  5:293659/1‑148 (MQ=255)
                                                                                     aCAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCg                                                                                   <  3:147898/119‑1 (MQ=255)
                                                                                     aCAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCg                                                                                   >  4:147898/1‑119 (MQ=255)
                                                                                       aaCGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGt                                                                                                  >  4:23036/1‑102 (MQ=255)
                                                                                       aaCGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGt                                                                                                  <  3:23036/102‑1 (MQ=255)
                                                                                                   aCGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAaca                                       >  4:60513/1‑149 (MQ=255)
                                                                                                     gccgcACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACAcc                                     <  1:343791/149‑1 (MQ=255)
                                                                                                            aTTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTctgct                              >  6:83762/1‑149 (MQ=255)
                                                                                                               ggtggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCtgctgc                            <  4:167917/148‑1 (MQ=255)
                                                                                                                gtggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGcac                          <  4:112218/149‑1 (MQ=255)
                                                                                                                gtggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGca                           >  2:245638/1‑148 (MQ=255)
                                                                                                                 tggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAacac                                      >  8:312102/1‑136 (MQ=255)
                                                                                                                 tggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAacac                                      <  7:312102/136‑1 (MQ=255)
                                                                                                                  ggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCg                                                                                   >  8:314280/1‑90 (MQ=255)
                                                                                                                  ggtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCg                                                                                   <  7:314280/90‑1 (MQ=255)
                                                                                                                   gtTCCACCGCCGAAGCCCAGTCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCACACACCGTCTGCTGCACAtt                       >  3:24360/1‑149 (MQ=255)
                                                                                                                   gtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACAtt                       >  8:187421/1‑149 (MQ=255)
                                                                                                                   gtTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACAt                        >  2:331377/1‑148 (MQ=255)
                                                                                                                      ccACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTctgct                              >  6:52863/1‑139 (MQ=255)
                                                                                                                      ccACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTctgct                              <  5:52863/139‑1 (MQ=255)
                                                                                                                      ccACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCCCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCAc                    >  6:188269/1‑149 (MQ=255)
                                                                                                                       cACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCAc                    >  2:130730/1‑148 (MQ=255)
                                                                                                                        aCCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACg                   >  7:205396/1‑148 (MQ=255)
                                                                                                                         ccgccgAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCTACTTCCGGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTctg                                <  5:268840/134‑1 (MQ=255)
                                                                                                                         ccgccgAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGACAGCGCACCCAGGCAAACACCGTctg                                >  6:268840/1‑134 (MQ=255)
                                                                                                                           gccgAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCAGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGaaaa               <  1:245638/149‑1 (MQ=255)
                                                                                                                                 ttccAGGCCGATGTTGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATccc         >  5:363924/3‑149 (MQ=255)
                                                                                                                                  cccAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCg        >  5:43553/1‑149 (MQ=255)
                                                                                                                                  cccAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCg        >  7:244291/1‑149 (MQ=255)
                                                                                                                                  cccAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCg        <  6:43553/149‑1 (MQ=255)
                                                                                                                                   ccAGGCCGATGTTGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTc                      <  5:42735/134‑1 (MQ=255)
                                                                                                                                   ccAGGCCGATGTTGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTc                      >  6:102468/1‑134 (MQ=255)
                                                                                                                                   ccAGGCCGATGTTGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTc                      <  5:102468/134‑1 (MQ=255)
                                                                                                                                   ccAGGCCGATGTTGGCCTGGAAGTGGCAGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTc                      >  6:42735/1‑134 (MQ=255)
                                                                                                                                   ccAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCgg       >  7:296763/1‑149 (MQ=255)
                                                                                                                                     aGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGc     <  2:173937/149‑1 (MQ=255)
                                                                                                                                     aGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGc     >  5:114230/1‑149 (MQ=255)
                                                                                                                                       gCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGcac                          <  6:116068/126‑1 (MQ=255)
                                                                                                                                       gCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGcac                          >  5:116068/1‑126 (MQ=255)
                                                                                                                                       gCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGCGt   <  1:331377/149‑1 (MQ=255)
                                                                                                                                        ccAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGCGTg  <  8:205396/149‑1 (MQ=255)
                                                                                                                                        ccAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGCGTg  <  3:206342/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGTGGTTCCACCGCCGAAGCCCAGGCCAACATCGGCCTGGAAGTGGCCGAGAAACTGGTCAAGTACAGCGACAACGGTACCTCGGTGTCCTCGGTCAACTTCCCGGAAGTGGCCCTGCCAGCGCACCCAGGCAAACACCGTCTGCTGCACATTCACGAAAACATCCCGGGCGTG  >  NC_002947/5882208‑5882492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑