Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,882,701 G→C 9.3% intergenic (+32/+16) serA → / ← PP_5156 bifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,882,7010GC 9.3% 159.0 / 5.5 53intergenic (+32/+16)serA/PP_5156bifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base C (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

CCAACGAGAAAGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCC  >  NC_002947/5882557‑5882846
                                                                                                                                                 |                                                                                                                                                  
ccAACGAGAAAGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCtggtgg                                                                                                                                                <  6:185488/149‑1 (MQ=255)
   aCGAGAAAGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑Ct                                                                                                                                            <  2:273750/149‑1 (MQ=255)
       gaAAGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCATCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCt                                                                                                                                        <  7:292687/149‑1 (MQ=255)
        aaaGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCACCAGGGC‑CTCCCtt                                                                                                                                       <  3:169135/149‑1 (MQ=255)
         aaGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCttt                                                                                                                                      <  6:240694/149‑1 (MQ=255)
              ggTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTa                                                                                                                                 <  8:197832/149‑1 (MQ=255)
               gTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTAt                                                                                                                                <  7:166797/149‑1 (MQ=255)
                  aCGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTAttt                                                                                                                              <  1:185615/148‑1 (MQ=255)
                       gaaaTCGATGATGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACtt                                                                                                                         >  6:167050/3‑148 (MQ=255)
                            cGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCg                                                                                                                   >  7:17306/1‑149 (MQ=255)
                            cGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCg                                                                                                                   >  6:168814/1‑149 (MQ=255)
                               tGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAAGACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGt                                                                                                                  >  1:232640/1‑148 (MQ=255)
                                    aCGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGAtcttc                                                                                                           >  1:191967/1‑149 (MQ=255)
                                       ccGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCttcttc                                                                                                        <  5:167050/149‑1 (MQ=255)
                                          aGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTtcgac                                                                                                     <  2:232640/149‑1 (MQ=255)
                                              cTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCACCAGGGC‑CTCCCttttt                                                                                                                                    >  7:333061/1‑114 (MQ=38)
                                              cTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCACCAGGGC‑CTCCCttttt                                                                                                                                    <  8:333061/114‑1 (MQ=38)
                                               tCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTtcgactcgac                                                                                                >  3:246419/1‑149 (MQ=255)
                                                 ggACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGc                                                                                              <  3:203791/149‑1 (MQ=255)
                                                  gACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTc                                                                                                                        >  3:342117/1‑122 (MQ=255)
                                                  gACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTc                                                                                                                        <  4:342117/122‑1 (MQ=255)
                                                     cTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAgg                                                                                          >  2:199819/1‑149 (MQ=255)
                                                             ggAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATg                                                                                  >  4:63424/1‑149 (MQ=255)
                                                              gAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATgg                                                                                 >  7:156890/1‑149 (MQ=255)
                                                                aaa‑aCTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGca                                                                               <  6:359427/149‑1 (MQ=255)
                                                                aaa‑aCTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGca                                                                               <  5:168814/149‑1 (MQ=255)
                                                                aaa‑aCTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGca                                                                               >  2:124466/1‑149 (MQ=255)
                                                                        cAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATa                                                                        <  4:246419/149‑1 (MQ=255)
                                                                         aaCAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATAc                                                                       >  3:180759/1‑149 (MQ=255)
                                                                            aGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTgg                                                                    >  4:133313/1‑149 (MQ=255)
                                                                             ggTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCTACTCGACGCTAGGCTTGAATGGcaca                                                                             >  4:108875/1‑139 (MQ=255)
                                                                             ggTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCTACTCGACGCTAGGCTTGAATGGcaca                                                                             <  3:108875/139‑1 (MQ=255)
                                                                                         cATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTgg                                                                    >  8:297241/1‑136 (MQ=255)
                                                                                         cATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTgg                                                                    <  7:297241/136‑1 (MQ=255)
                                                                                             cGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTg                                                   <  8:17306/149‑1 (MQ=255)
                                                                                               ttCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAAt                                                                                   <  1:288063/115‑1 (MQ=255)
                                                                                               ttCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAAt                                                                                   >  2:288063/1‑115 (MQ=255)
                                                                                               ttCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCa                                                 <  4:180759/149‑1 (MQ=255)
                                                                                                  gcgcgTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACACTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAgg                                               <  2:191967/148‑1 (MQ=255)
                                                                                                               tAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCgg                                 <  8:156890/149‑1 (MQ=255)
                                                                                                               tAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCgg                                 >  2:214758/1‑149 (MQ=255)
                                                                                                               tAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCgg                                 >  8:75084/1‑149 (MQ=255)
                                                                                                                aaGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCggg                                <  1:124466/149‑1 (MQ=255)
                                                                                                                       cTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAggg                         <  3:133313/149‑1 (MQ=255)
                                                                                                                        atctggTATGAAAAAGGCAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGt                        <  2:2818/145‑1 (MQ=255)
                                                                                                                            ggTATGAAAAAGGGAGGCCCGGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATg                    >  5:206848/1‑149 (MQ=255)
                                                                                                                             gTATGAAAAAGGGAGGCCCTGGTGGCGCTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGt                        >  1:2818/1‑145 (MQ=255)
                                                                                                                               taaaaaaaGGGAGGCCACCAGGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAgg                                               >  7:111478/4‑119 (MQ=38)
                                                                                                                               taaaaaaaGGGAGGCCACCAGGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAgg                                               <  8:111478/116‑1 (MQ=38)
                                                                                                                                tGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTg                 <  1:199819/148‑1 (MQ=255)
                                                                                                                                      aGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCgg          >  6:105257/1‑149 (MQ=255)
                                                                                                                                              ccTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCtgt     >  6:120766/1‑146 (MQ=255)
                                                                                                                                              ccTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCtgt     <  5:120766/146‑1 (MQ=255)
                                                                                                                                              ccTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGcc  >  3:165341/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCAACGAGAAAGTCGGTTACGTGGTAATCGATGTTGACGCCGAGTACTCGGACCTGGCGCAGGAAAA‑ACTGCAACAGGTCAAAGGCACCATCCGTTCGCGCGTCCTGTTCTAAGGTCGCTGGCGGTATGAAAAAGGGAGGCCCTGGTGGC‑CTCCCTTTTTTTATTTCACTTCAACCGTGATCTTCTTCGACTCGACGCTAGGCTTGAATGGCACATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCC  >  NC_002947/5882557‑5882846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑