Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,883,595 C→G 13.7% A60G (GCC→GGC)  PP_5157 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,883,5950CG13.7% 129.7 / 13.1 51A60G (GCC→GGC) PP_5157hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/19);  new base G (3/4);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

CTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGC  >  NC_002947/5883450‑5883740
                                                                                                                                                 |                                                                                                                                                 
cTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCt                                                                                                                                                >  1:217710/1‑149 (MQ=255)
 tGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGAAAGGTTTCGACATCGAATTTGGCCAGGCCCTg                                                                                                                                               >  1:241739/1‑149 (MQ=255)
      aGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCaa                                                                                                                                          <  2:290751/149‑1 (MQ=255)
       gTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGGCCTGGCCaaa                                                                                                                                         <  1:249991/149‑2 (MQ=255)
       gTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAg                                                                                                                                         >  2:31165/1‑149 (MQ=255)
        tCCATTGGCTCAGGCGGAGCGGATCGACGAAATCGCCGACCGGGGCGAACTGCGCAGTGCCGTACCGGCCGCGAACTCGCCATCCGCCTTCACGCAAGACGACCACCTGACAGGTTTCGCCATCGCATTTGGGCAGGCCGGGGCCAAgg                                                                                                                                        >  8:240717/1‑149 (MQ=255)
          cATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGATCTGCGCATTGCCGTACAGGCCGATAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGGCCTGGCCaaatt                                                                                                                                       <  4:300370/148‑4 (MQ=255)
          cATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGGCCTGGCCaaatt                                                                                                                                       <  2:246976/148‑4 (MQ=255)
           aTTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAAc                                                                                                                                     <  6:133299/149‑1 (MQ=255)
                 tCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGAtt                                                                                                                               >  6:163923/1‑149 (MQ=255)
                       ggAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTgcgat                                                                                                                         >  6:157331/1‑147 (MQ=255)
                         aGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGcc                                                                                                                       >  1:245793/1‑149 (MQ=255)
                                  acgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgtc                                                                                                              <  2:245793/149‑1 (MQ=255)
                                     aaaTCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTTACAGGTTTCGACATCTAATTTGGCCACGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGaa                                                                                                           <  1:67812/149‑1 (MQ=255)
                                     aaaTCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAACGAACTGGATTTGCGCGCCGAGTTCGTCGaa                                                                                                           <  1:31165/149‑1 (MQ=255)
                                        tCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGcc                                                                                                        >  3:247358/1‑149 (MQ=255)
                                                 ggggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTAcc                                                                                               <  1:294688/149‑1 (MQ=255)
                                                      gAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGt                                                                                          <  2:217710/149‑1 (MQ=255)
                                                                ttGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgt                                                                                <  4:247358/149‑1 (MQ=255)
                                                                     gTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGaa                                                                                                           >  2:175812/1‑117 (MQ=255)
                                                                     gTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                           <  3:71107/149‑1 (MQ=255)
                                                                             cGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGc                                                                    >  2:183180/1‑148 (MQ=255)
                                                                              gagaACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCaa                                                                  <  1:183180/149‑1 (MQ=255)
                                                                                gaACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCgaga                                                                         <  7:60534/140‑1 (MQ=255)
                                                                                gaACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCgaga                                                                         >  8:60534/1‑140 (MQ=255)
                                                                                             gCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCAt                                                    >  4:8296/1‑148 (MQ=255)
                                                                                                ttCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtgt                                                                              >  4:203255/1‑119 (MQ=255)
                                                                                                ttCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtgt                                                                              <  3:203255/119‑1 (MQ=255)
                                                                                                ttCAAGAAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGACCTGGCCAAGGAACTGGATATGAGCTCCTAGTTCTTAGAAGCCAAGACTACCCAAGAGATGGCGTGTGTCGAGAGTCGCAAGTAATACATCGCATTCAc                                                >  4:325010/1‑149 (MQ=255)
                                                                                                   aagcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                           >  6:207703/1‑119 (MQ=255)
                                                                                                             acccccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGACAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGaa                                   <  7:240717/149‑1 (MQ=255)
                                                                                                              ccaccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtgt                                                                              >  1:294273/1‑105 (MQ=255)
                                                                                                              ccaccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtgt                                                                              <  2:294273/105‑1 (MQ=255)
                                                                                                               caccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCGCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGt                                                                       <  6:147217/111‑1 (MQ=255)
                                                                                                               caccTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGt                                                                       >  5:147217/1‑111 (MQ=255)
                                                                                                                     aCAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTca                            <  3:8296/148‑1 (MQ=255)
                                                                                                                              gACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACgg                  >  6:53390/1‑149 (MQ=255)
                                                                                                                               aCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                          <  8:95291/124‑1 (MQ=255)
                                                                                                                               aCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTc                                          >  7:95291/1‑124 (MQ=255)
                                                                                                                                 aTCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGccc               >  5:213264/1‑149 (MQ=255)
                                                                                                                                    ttccttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGc                       >  2:162672/5‑138 (MQ=255)
                                                                                                                                    ttccttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGc                       <  1:162672/134‑1 (MQ=255)
                                                                                                                                        ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAcc                                               >  6:46360/1‑110 (MQ=255)
                                                                                                                                        ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAt        >  6:183362/1‑149 (MQ=255)
                                                                                                                                          ggCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATg       >  4:65951/1‑148 (MQ=255)
                                                                                                                                            ccAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTca                            <  7:133025/125‑1 (MQ=255)
                                                                                                                                            ccAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTca                            >  8:133025/1‑125 (MQ=255)
                                                                                                                                            ccAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTaa    <  6:213264/149‑1 (MQ=255)
                                                                                                                                            ccAGGCCCTGACCAAGGAACTGGATTTGCGCGCCGAGATCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAAGt      >  7:40362/1‑147 (MQ=255)
                                                                                                                                             cAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAg   >  2:263592/1‑149 (MQ=255)
                                                                                                                                              aGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGAGGGTGTCGAGAGTGGCAAGTTCGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGAGGTAAGc  >  1:268487/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGC  >  NC_002947/5883450‑5883740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑