| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,909,094 | C→T | 6.8% | A43T (GCC→ACC) | spuD ← | spermidine/putrescine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,909,094 | 0 | C | T | 6.8% | 173.0 / 2.5 | 59 | A43T (GCC→ACC) | spuD | spermidine/putrescine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (29/26); new base T (2/2); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CGCCTTGATCTGCTTGGCCAGGAAATTGTTCGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTA > NC_002947/5908948‑5909242 | cgccTTGATCTGCTTGGCCAGGAAATTGTTCGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTTCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGa > 2:338384/1‑149 (MQ=255) ccTTGATCTGCTTGGCCAGGAAATTGTTCGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATg < 4:342673/149‑1 (MQ=255) tCTGCTTGGCCAGGAAATTGTTCGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTcc > 3:192279/1‑149 (MQ=255) aaTTGTTCGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGATGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTcc > 8:307078/1‑149 (MQ=255) cGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGt > 3:219494/1‑149 (MQ=255) aaGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTta < 4:346437/149‑1 (MQ=255) ggCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTTCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTtata < 1:338384/149‑1 (MQ=255) gCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGTGATGGTGTCCGGAGCGATGTAGTCCGACCAGt < 4:317864/144‑1 (MQ=255) gCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGTGATGGTGTCCGGAGCGATGTAGTCCGACCAGt < 4:312821/144‑1 (MQ=255) gCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGTGATGGTGTCCGGAGCGATGTAGTCCGACCAGt > 3:317864/1‑144 (MQ=255) gCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGTGATGGTGTCCGGAGCGATGTAGTCCGACCAGt > 3:312821/1‑144 (MQ=255) cACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGa < 3:248715/149‑1 (MQ=255) acgacgATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGAc > 4:42729/1‑149 (MQ=255) acgaTGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGtt < 2:213671/149‑1 (MQ=255) cATAGCCCGACTAGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGGCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACt < 8:162928/149‑1 (MQ=255) aTAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACt < 4:192279/148‑1 (MQ=255) tAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACttt < 3:42729/149‑1 (MQ=255) aGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGa < 5:322461/124‑1 (MQ=255) aGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGa > 6:322461/1‑124 (MQ=255) cccGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTc > 2:251994/1‑149 (MQ=255) cccGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTc < 1:251994/149‑1 (MQ=255) cccGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTc > 6:11120/1‑149 (MQ=255) gACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACtt > 4:145891/1‑142 (MQ=255) gACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACtt < 3:145891/142‑1 (MQ=255) gACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCtt > 4:329548/1‑148 (MQ=255) gACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCtt > 3:277008/1‑148 (MQ=255) gcctgccAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGc < 4:219494/147‑1 (MQ=255) agcagcTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGaaaa > 7:76272/1‑145 (MQ=255) agcagcTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGc < 4:154523/149‑1 (MQ=255) gcagcTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTc < 2:2973/122‑1 (MQ=255) gcagcTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTc > 1:2973/1‑122 (MQ=255) ttCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATgg > 6:113774/1‑149 (MQ=255) cAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCTAACTTGGCGATGGTGTCCGGAGCGATGTAGTcc < 1:47276/91‑1 (MQ=255) cAGGGTTTCGTTGCTGTCGAAGACCTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTcc > 2:47276/1‑91 (MQ=255) aGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGa < 4:247289/137‑1 (MQ=255) aGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGa > 3:247289/1‑137 (MQ=255) ggTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAg > 6:337131/1‑131 (MQ=255) ggTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAg < 5:337131/131‑1 (MQ=255) tGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTTGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGACACCCATCAGggcggcg > 6:330712/1‑149 (MQ=255) tCGAAGACGTCGTACTTGAACTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTGCAACACTTTGAATTCAGCCTGAACAGCGGCGGCCATGGCACCAATCAGggcggcgtc > 8:9497/1‑147 (MQ=255) gAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATc < 3:77976/136‑1 (MQ=255) gAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATc > 4:77976/1‑136 (MQ=255) gtcgtACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTTGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATgg > 1:212186/1‑122 (MQ=255) gtcgtACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATgg < 2:212186/122‑1 (MQ=255) ttGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGggcggcg > 8:59132/1‑132 (MQ=255) ttGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGggcggcg < 7:59132/132‑1 (MQ=255) ttGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTACACCAGTTTGTCTTCAGGCTGAACAGCGGAGGCCATGGCTCCCAGCAGGGCGGCGGCCCGCACCGTCTTGcc > 8:247351/1‑149 (MQ=255) ccTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCAtt < 5:11120/149‑1 (MQ=255) cTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCAttt > 7:188612/1‑149 (MQ=255) tCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTAtt > 2:307488/1‑148 (MQ=255) tCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTc < 7:307078/149‑1 (MQ=255) ccTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGATCGATGTAGTCCGACCATTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACATCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCAt < 7:9497/149‑1 (MQ=255) cTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGTGGCCAGCAAc > 2:331462/1‑121 (MQ=255) cTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCATGCGTAATg < 4:277008/149‑1 (MQ=255) tcGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCATGCGTAATGCTc > 3:95387/1‑148 (MQ=255) gAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCAt < 6:67611/135‑1 (MQ=255) gAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCAt > 5:67611/1‑135 (MQ=255) ttGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCa > 4:164726/1‑121 (MQ=255) ttGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCa < 3:164726/121‑1 (MQ=255) cGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGACACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTttta < 5:330712/149‑1 (MQ=255) | CGCCTTGATCTGCTTGGCCAGGAAATTGTTCGAAGGCACGACGATGTCATAGCCCGACTTGCCTGCCAGCAGCTTGGCTTCCAGGGTTTCGTTGCTGTCGAAGACGTCGTACTTGACCTTGATCCCTGTCTGCTTCTCGAACTTGGCGATGGTGTCCGGAGCGATGTAGTCCGACCAGTTATAGACGTTCAACACTTTGTCTTCAGCCTGAACAGCGGAGGCCATGGCACCCATCAGGGCGGCGGCCAGCAACGTCTTGCCCATTTTATTCATGCGTAATGCTCCAGAATTTTTA > NC_002947/5908948‑5909242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |