Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,932,011 A→G 8.8% L20S (TTG→TCG) ‡ ubiH ← 2‑octaprenyl‑6‑methoxyphenyl hydroxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,932,0110AG 8.8% 151.6 / 4.8 57L20S (TTG→TCG) ‡ubiH2‑octaprenyl‑6‑methoxyphenyl hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base G (3/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CCAGTTGCTGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTCACAACCTTGCC  >  NC_002947/5931868‑5932157
                                                                                                                                               |                                                                                                                                                  
ccAGTTGCTGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGc                                                                                                                                                >  5:286441/1‑148 (MQ=255)
 cAGTTGCTGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGc                                                                                                                                                >  4:77556/1‑147 (MQ=255)
 cAGTTGCTGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCa                                                                                                                                              <  5:197684/149‑1 (MQ=255)
    ttGCTGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGc                                                                                                                                           <  2:205022/149‑1 (MQ=255)
      gCTGATAAATCTGCTGTGTGCCTAATGACTATGCCGAAGCGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCTAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCt                                                                                                                                          <  7:331065/148‑1 (MQ=255)
        tGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAgg                                                                                                                                            >  5:49383/1‑144 (MQ=255)
        tGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAgg                                                                                                                                            <  3:151093/144‑1 (MQ=255)
        tGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAgg                                                                                                                                            >  4:151093/1‑144 (MQ=255)
        tGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAgg                                                                                                                                            <  6:49383/144‑1 (MQ=255)
          aTAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTgcg                                                                                                                                      <  3:77556/148‑1 (MQ=255)
            aaaTCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGcc                                                                                                                                    >  2:114256/1‑148 (MQ=255)
              aTCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAAc                                                                                                                                 <  6:286441/149‑1 (MQ=255)
               tCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAAc                                                                                                                                 >  5:55444/1‑148 (MQ=255)
                   ctgGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAg                                                                                                                              <  3:122959/147‑1 (MQ=255)
                         gCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCAccgc                                                                                                                      <  1:114256/149‑1 (MQ=255)
                                        cGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGt                                                                                                       >  2:153201/1‑149 (MQ=255)
                                          aaGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCgat                                                                                                                  >  5:56896/1‑136 (MQ=255)
                                          aaGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCgat                                                                                                                  <  6:56896/136‑1 (MQ=255)
                                                gcgcATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGcc                                                                                                           <  2:73919/137‑1 (MQ=255)
                                                gcgcATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGcc                                                                                                           >  1:73919/1‑137 (MQ=255)
                                                gcgcATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCgg                                                                                               <  6:117429/149‑1 (MQ=255)
                                                            tgggctggTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACgg                                                                                   >  1:332016/1‑149 (MQ=255)
                                                                    tAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCgg                                                                           <  2:332016/149‑1 (MQ=255)
                                                                         gTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGc                                                                      >  4:95597/1‑149 (MQ=255)
                                                                          tCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCTAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGcc                                                                     >  4:196554/1‑149 (MQ=255)
                                                                          tCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCg                                                                            <  6:219618/142‑1 (MQ=255)
                                                                          tCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCg                                                                            >  5:219618/1‑142 (MQ=255)
                                                                            aCCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCAc                                                                                     >  6:212589/1‑131 (MQ=255)
                                                                            aCCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCAc                                                                                     <  5:212589/131‑1 (MQ=255)
                                                                             ccGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCg                                                                            >  7:319903/1‑139 (MQ=255)
                                                                             ccGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCg                                                                            <  8:319903/139‑1 (MQ=255)
                                                                             ccGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTg                                                                   >  1:298583/1‑148 (MQ=255)
                                                                              cGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCa                                                                 >  1:259477/1‑149 (MQ=255)
                                                                               gggggCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCAt                                                                <  3:95597/149‑1 (MQ=255)
                                                                                     gAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAgcgc                                                          >  5:126829/1‑149 (MQ=255)
                                                                                         ggCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTc                                                       >  1:174304/1‑148 (MQ=255)
                                                                                          gCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGa                                                     >  1:334403/1‑149 (MQ=255)
                                                                                                  agcagGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGc                                              >  5:139558/1‑148 (MQ=255)
                                                                                                     agGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGAcc                                          <  6:55444/149‑1 (MQ=255)
                                                                                                       gATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCg                                         >  8:315922/1‑148 (MQ=255)
                                                                                                              cAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCgg                                  <  2:334403/148‑1 (MQ=255)
                                                                                                                gCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCATACGCGGCAc                               <  2:259477/149‑1 (MQ=255)
                                                                                                                           tGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGt                      >  7:98344/1‑147 (MQ=255)
                                                                                                                             gcgcCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCa                    <  1:153201/147‑1 (MQ=255)
                                                                                                                             cgttggcccTGCA‑GGCCGGCGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGa                 >  1:308883/8‑149 (MQ=255)
                                                                                                                             agttggcccTGCA‑GGCCGGCGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGa                 >  3:279269/8‑149 (MQ=255)
                                                                                                                                tggcccTGCA‑GGCCGGCGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGAtt               >  8:236980/5‑148 (MQ=255)
                                                                                                                                ccGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGAtt               >  5:71728/1‑149 (MQ=255)
                                                                                                                                 cGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCAt                                                                <  5:168489/99‑1 (MQ=255)
                                                                                                                                 cGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCAt                                                                >  6:168489/1‑99 (MQ=255)
                                                                                                                                  ggCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTc             <  7:315922/149‑1 (MQ=255)
                                                                                                                                  gcccTGCA‑GGCCGGCGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTc             <  2:308883/146‑1 (MQ=255)
                                                                                                                                  gcccTGCA‑GGCCGGCGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTc             <  4:279269/146‑1 (MQ=255)
                                                                                                                                     cTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTcac           >  2:343485/1‑148 (MQ=255)
                                                                                                                                            gCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTCACAACCTTGc   >  5:280355/1‑149 (MQ=255)
                                                                                                                                             ccAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTCACAACCTTGcc  <  6:126829/149‑1 (MQ=255)
                                                                                                                                              cAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAg                   <  6:16014/131‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CCAGTTGCTGATAAATCTGCTGGGTGCCGAATGACAATGCCGAAGAGCGCGCATCATAGCTGGGCTGGTAGCTGTCACCGGGGGCGAACGGCTCGATCAGCAGGATCTTCCAGCCGCGCGCCCTGGCGCCGGCCTGCAGGGCCAACGCCAGGCTTGCGCCAACCAGGCCACCGCCGATGATCGCCAGGTTTACCCGGCTCATGCCACGGCCTTGCGGGCAGCCTGCATCAGCGCCTCGATCTCGGCGACCGTACGCGGCACGCCCGAAGTCAGGATTTCACAACCTTGCC  >  NC_002947/5931868‑5932157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑